[2023-06-18 21:21:00,528] [INFO] DFAST_QC pipeline started.
[2023-06-18 21:21:00,533] [INFO] DFAST_QC version: 0.5.7
[2023-06-18 21:21:00,533] [INFO] DQC Reference Directory: /var/lib/cwl/stg623ef093-5f7e-4e21-94ef-918b64654df2/dqc_reference
[2023-06-18 21:21:01,853] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-18 21:21:01,854] [INFO] Task started: Prodigal
[2023-06-18 21:21:01,854] [INFO] Running command: gunzip -c /var/lib/cwl/stg5e66cb6a-6184-4e6e-b975-44358abffe4b/GCA_937925005.1_S1_Bin_METABAT_191_sub_1_genomic.fna.gz | prodigal -d GCA_937925005.1_S1_Bin_METABAT_191_sub_1_genomic.fna/cds.fna -a GCA_937925005.1_S1_Bin_METABAT_191_sub_1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-18 21:21:04,310] [INFO] Task succeeded: Prodigal
[2023-06-18 21:21:04,310] [INFO] Task started: HMMsearch
[2023-06-18 21:21:04,311] [INFO] Running command: hmmsearch --tblout GCA_937925005.1_S1_Bin_METABAT_191_sub_1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg623ef093-5f7e-4e21-94ef-918b64654df2/dqc_reference/reference_markers.hmm GCA_937925005.1_S1_Bin_METABAT_191_sub_1_genomic.fna/protein.faa > /dev/null
[2023-06-18 21:21:04,517] [INFO] Task succeeded: HMMsearch
[2023-06-18 21:21:04,519] [WARNING] Found 4/6 markers. [/var/lib/cwl/stg5e66cb6a-6184-4e6e-b975-44358abffe4b/GCA_937925005.1_S1_Bin_METABAT_191_sub_1_genomic.fna.gz]
[2023-06-18 21:21:04,545] [INFO] Query marker FASTA was written to GCA_937925005.1_S1_Bin_METABAT_191_sub_1_genomic.fna/markers.fasta
[2023-06-18 21:21:04,545] [INFO] Task started: Blastn
[2023-06-18 21:21:04,546] [INFO] Running command: blastn -query GCA_937925005.1_S1_Bin_METABAT_191_sub_1_genomic.fna/markers.fasta -db /var/lib/cwl/stg623ef093-5f7e-4e21-94ef-918b64654df2/dqc_reference/reference_markers.fasta -out GCA_937925005.1_S1_Bin_METABAT_191_sub_1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-18 21:21:05,662] [INFO] Task succeeded: Blastn
[2023-06-18 21:21:05,671] [INFO] Selected 6 target genomes.
[2023-06-18 21:21:05,671] [INFO] Target genome list was writen to GCA_937925005.1_S1_Bin_METABAT_191_sub_1_genomic.fna/target_genomes.txt
[2023-06-18 21:21:05,707] [INFO] Task started: fastANI
[2023-06-18 21:21:05,707] [INFO] Running command: fastANI --query /var/lib/cwl/stg5e66cb6a-6184-4e6e-b975-44358abffe4b/GCA_937925005.1_S1_Bin_METABAT_191_sub_1_genomic.fna.gz --refList GCA_937925005.1_S1_Bin_METABAT_191_sub_1_genomic.fna/target_genomes.txt --output GCA_937925005.1_S1_Bin_METABAT_191_sub_1_genomic.fna/fastani_result.tsv --threads 1
[2023-06-18 21:21:07,946] [INFO] Task succeeded: fastANI
[2023-06-18 21:21:07,947] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg623ef093-5f7e-4e21-94ef-918b64654df2/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-18 21:21:07,948] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg623ef093-5f7e-4e21-94ef-918b64654df2/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-18 21:21:07,950] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-18 21:21:07,950] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-18 21:21:07,950] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-18 21:21:07,954] [INFO] DFAST Taxonomy check result was written to GCA_937925005.1_S1_Bin_METABAT_191_sub_1_genomic.fna/tc_result.tsv
[2023-06-18 21:21:07,955] [INFO] ===== Taxonomy check completed =====
[2023-06-18 21:21:07,956] [INFO] ===== Start completeness check using CheckM =====
[2023-06-18 21:21:07,956] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg623ef093-5f7e-4e21-94ef-918b64654df2/dqc_reference/checkm_data
[2023-06-18 21:21:07,960] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-18 21:21:07,987] [INFO] Task started: CheckM
[2023-06-18 21:21:07,988] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_937925005.1_S1_Bin_METABAT_191_sub_1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_937925005.1_S1_Bin_METABAT_191_sub_1_genomic.fna/checkm_input GCA_937925005.1_S1_Bin_METABAT_191_sub_1_genomic.fna/checkm_result
[2023-06-18 21:21:24,600] [INFO] Task succeeded: CheckM
[2023-06-18 21:21:24,602] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 56.94%
Contamintation: 5.56%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-18 21:21:24,626] [INFO] ===== Completeness check finished =====
[2023-06-18 21:21:24,627] [INFO] ===== Start GTDB Search =====
[2023-06-18 21:21:24,627] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_937925005.1_S1_Bin_METABAT_191_sub_1_genomic.fna/markers.fasta)
[2023-06-18 21:21:24,628] [INFO] Task started: Blastn
[2023-06-18 21:21:24,628] [INFO] Running command: blastn -query GCA_937925005.1_S1_Bin_METABAT_191_sub_1_genomic.fna/markers.fasta -db /var/lib/cwl/stg623ef093-5f7e-4e21-94ef-918b64654df2/dqc_reference/reference_markers_gtdb.fasta -out GCA_937925005.1_S1_Bin_METABAT_191_sub_1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-18 21:21:25,061] [INFO] Task succeeded: Blastn
[2023-06-18 21:21:25,067] [INFO] Selected 8 target genomes.
[2023-06-18 21:21:25,067] [INFO] Target genome list was writen to GCA_937925005.1_S1_Bin_METABAT_191_sub_1_genomic.fna/target_genomes_gtdb.txt
[2023-06-18 21:21:25,090] [INFO] Task started: fastANI
[2023-06-18 21:21:25,090] [INFO] Running command: fastANI --query /var/lib/cwl/stg5e66cb6a-6184-4e6e-b975-44358abffe4b/GCA_937925005.1_S1_Bin_METABAT_191_sub_1_genomic.fna.gz --refList GCA_937925005.1_S1_Bin_METABAT_191_sub_1_genomic.fna/target_genomes_gtdb.txt --output GCA_937925005.1_S1_Bin_METABAT_191_sub_1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-18 21:21:27,568] [INFO] Task succeeded: fastANI
[2023-06-18 21:21:27,572] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-06-18 21:21:27,573] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_011047235.1	s__SpSt-318 sp011047235	81.7543	184	403	d__Bacteria;p__SpSt-318;c__SpSt-318;o__SpSt-318;f__SpSt-318;g__SpSt-318	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-06-18 21:21:27,575] [INFO] GTDB search result was written to GCA_937925005.1_S1_Bin_METABAT_191_sub_1_genomic.fna/result_gtdb.tsv
[2023-06-18 21:21:27,575] [INFO] ===== GTDB Search completed =====
[2023-06-18 21:21:27,578] [INFO] DFAST_QC result json was written to GCA_937925005.1_S1_Bin_METABAT_191_sub_1_genomic.fna/dqc_result.json
[2023-06-18 21:21:27,579] [INFO] DFAST_QC completed!
[2023-06-18 21:21:27,579] [INFO] Total running time: 0h0m27s