<?xml version="1.0" ?>
<BioSampleSet><BioSample access="public" publication_date="2023-01-03T00:00:00.000" last_update="2023-04-12T11:27:12.000" submission_date="2023-01-04T08:30:43.703" id="32559659" accession="SAMEA14082363">   <Ids>     <Id db="BioSample" is_primary="1">SAMEA14082363</Id>     <Id db="SRA">ERS11685499</Id>   </Ids>   <Description>     <Title>Metagenome-assembled genome: SRR6231178_bin.58_CONCOCT_v1.1_MAG</Title>     <Organism taxonomy_id="290433" taxonomy_name="uncultured Helicobacteraceae bacterium">       <OrganismName>uncultured Helicobacteraceae bacterium</OrganismName>     </Organism>     <Comment>       <Paragraph>This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR6231178 of study SRP121672.</Paragraph>     </Comment>   </Description>   <Owner>     <Name>EBI</Name>   </Owner>   <Models>     <Model>Generic</Model>   </Models>   <Package display_name="Generic">Generic.1.0</Package>   <Attributes>     <Attribute attribute_name="ENA-CHECKLIST">ERC000047</Attribute>     <Attribute attribute_name="ENA-FIRST-PUBLIC">2023-01-03</Attribute>     <Attribute attribute_name="ENA-LAST-UPDATE">2023-01-03</Attribute>     <Attribute attribute_name="External Id">SAMEA14082363</Attribute>     <Attribute attribute_name="INSDC center alias">EBI</Attribute>     <Attribute attribute_name="INSDC center name">European Bioinformatics Institute</Attribute>     <Attribute attribute_name="INSDC first public">2023-01-03T00:32:25Z</Attribute>     <Attribute attribute_name="INSDC last update">2023-01-03T00:32:25Z</Attribute>     <Attribute attribute_name="INSDC status">public</Attribute>     <Attribute attribute_name="Submitter Id">SRR6231178_bin.58_CONCOCT_v1.1_MAG</Attribute>     <Attribute attribute_name="assembly quality">Many fragments with little to no review of assembly other than reporting of standard assembly statistics</Attribute>     <Attribute attribute_name="assembly software">metaspadesv3.11.1</Attribute>     <Attribute attribute_name="binning parameters">Default</Attribute>     <Attribute attribute_name="binning software">CONCOCT v1.1</Attribute>     <Attribute attribute_name="broker name">EMG broker account, EMBL-EBI</Attribute>     <Attribute attribute_name="collection date" harmonized_name="collection_date" display_name="collection date">2016-04-26</Attribute>     <Attribute attribute_name="completeness score">98.33</Attribute>     <Attribute attribute_name="completeness software">CheckM</Attribute>     <Attribute attribute_name="contamination score">2.34</Attribute>     <Attribute attribute_name="environment (biome)" harmonized_name="env_broad_scale" display_name="broad-scale environmental context">Engineered</Attribute>     <Attribute attribute_name="environment (feature)" harmonized_name="env_local_scale" display_name="local-scale environmental context">Engineered</Attribute>     <Attribute attribute_name="environment (material)" harmonized_name="env_medium" display_name="environmental medium">Engineered</Attribute>     <Attribute attribute_name="geographic location (country and/or sea)" harmonized_name="geo_loc_name" display_name="geographic location">USA</Attribute>     <Attribute attribute_name="geographic location (latitude)">35.15712</Attribute>     <Attribute attribute_name="geographic location (longitude)">-80.84963</Attribute>     <Attribute attribute_name="investigation type" harmonized_name="investigation_type" display_name="investigation type">metagenome-assembled genome</Attribute>     <Attribute attribute_name="isolation_source" harmonized_name="isolation_source" display_name="isolation source">metagenome</Attribute>     <Attribute attribute_name="metagenomic source">metagenome</Attribute>     <Attribute attribute_name="project name" harmonized_name="project_name" display_name="project name">Wastewater treatment plants (WWTPs) are thought to be potential incubators of antibiotic resistance. Persistence of commonly used antibiotics in wastewater may increase the potential for survival and horizontal transfer of adaptive genes in bacterial populations, some of which may pose a threat to human health. In this study, we assayed the concentrations of ten common antibiotics in wastewater influents, treatment stage influents and effluents, and upstream and downstream surface waters from two Charlotte area wastewater plants, including residential and hospital trunkline locations. We performed whole genome shotgun sequencing, to assay the taxonomic and resistome compositions at each of these sites across four time points during the late winter to mid-summer of 2016.</Attribute>     <Attribute attribute_name="sample derived from">SAMN07839306</Attribute>     <Attribute attribute_name="sample name" harmonized_name="sample_name" display_name="sample name">SRR6231178_bin.58_CONCOCT_v1.1_MAG</Attribute>     <Attribute attribute_name="scientific_name">uncultured Helicobacteraceae bacterium</Attribute>     <Attribute attribute_name="sequencing method">Illumina HiSeq 2500</Attribute>     <Attribute attribute_name="taxonomic identity marker">multi-marker approach</Attribute>   </Attributes>   <Status status="live" when="2023-01-05T08:28:45.583"/> </BioSample> </BioSampleSet>
