SAMEA14081904 ERS11685040 uncultured Microbacterium sp. This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR3901698 of study SRP056932. EBI Generic Generic.1.0 ERC000047 2023-01-03 2023-01-03 SAMEA14081904 EBI European Bioinformatics Institute 2023-01-03T00:32:45Z 2023-01-03T00:32:45Z public SRR3901698_bin.67_CONCOCT_v1.1_MAG Many fragments with little to no review of assembly other than reporting of standard assembly statistics metaspadesv3.12.0 Default CONCOCT v1.1 EMG broker account, EMBL-EBI 2014-04-04 95.2 CheckM 2.2 Engineered Engineered Engineered South Africa -33.9575 18.4606 metagenome-assembled genome metagenome metagenome Gold mining and other industrial activities make extensive use of cyanide (CN-) and produce large volumes of thiocyanate- (SCN-) contaminated wastewater that must be treated before reuse in mining processes. Furthermore, contaminated wastewater stored in tailings dams may pose an environmental threat through the potential for leakage into local groundwater. Microbial communities are often used for biodegradation and environmental remediation, however, little is known about the membership and metabolic potential of SCN-degrading consortia. Application and improvement of the remediation strategy will benefit from an understanding of organisms that can contribute to the breakdown of SCN- into sulfur, carbon and nitrogen compounds. To this end, we performed metagenomic analysis of samples from two laboratory-scale bioreactors used to study SCN- and CN- degradation by established microbial consortia. Community analysis revealed the dominance of Thiobacillus spp. whose genomes harbor a previously unreported operon for SCN- degradation. Metabolic predictions suggest that a large portion of each bioreactor community is autotrophic, relying not on molasses in reactor feed, but using energy gained from oxidation of the sulfur compounds produced during SCN- degradation. Heterotrophs compose a smaller portion of the reactor bacterial community, and predation by phage and eukaryotes is predicted to affect community dynamics. Furthermore, genes for ammonium oxidation and denitrification were detected, possibly allowing removal of nitrogen as required for complete remediation of wastewater. These findings suggest possible optimization strategies for reactor design such as improved aerobic/anaerobic partitioning and elimination of organic carbon from reactor feed. SAMN05389826 SRR3901698_bin.67_CONCOCT_v1.1_MAG uncultured Microbacterium sp. Illumina HiSeq 2500 multi-marker approach