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<BioSampleSet><BioSample access="public" publication_date="2023-01-18T00:00:00.000" last_update="2023-01-24T02:16:14.000" submission_date="2023-01-19T08:09:53.890" id="32790212" accession="SAMEA14073840">   <Ids>     <Id db="BioSample" is_primary="1">SAMEA14073840</Id>     <Id db="SRA">ERS11677072</Id>   </Ids>   <Description>     <Title>R1_Bin_METABAT__11_1</Title>     <Organism taxonomy_id="77133" taxonomy_name="uncultured bacterium">       <OrganismName>uncultured bacterium</OrganismName>     </Organism>     <Comment>       <Paragraph>Macroalgea were then put in sterile seawater and remaining bacteria were removed by ultrasonication (2x 60s, 50-60 kHz). Suspensions with removed bacteria of each sample were subsequently pooled with the previously obtained washing suspensions. Per sample, nearly 60 L of washing suspension was then filtered through 0.2 µm pore size polycarbonate membrane filters (Millipore). DNA was extracted from these filters, frozen at -80°C.</Paragraph>     </Comment>   </Description>   <Owner>     <Name>EBI</Name>   </Owner>   <Models>     <Model>Generic</Model>   </Models>   <Package display_name="Generic">Generic.1.0</Package>   <Attributes>     <Attribute attribute_name="ENA-CHECKLIST">ERC000011</Attribute>     <Attribute attribute_name="ENA-FIRST-PUBLIC">2023-01-18</Attribute>     <Attribute attribute_name="ENA-LAST-UPDATE">2023-01-18</Attribute>     <Attribute attribute_name="External Id">SAMEA14073840</Attribute>     <Attribute attribute_name="INSDC center alias">Marine College, Shandong University</Attribute>     <Attribute attribute_name="INSDC center name">marine college, shandong university</Attribute>     <Attribute attribute_name="INSDC first public">2023-01-18T00:18:46Z</Attribute>     <Attribute attribute_name="INSDC last update">2023-01-18T00:18:46Z</Attribute>     <Attribute attribute_name="INSDC status">public</Attribute>     <Attribute attribute_name="Submitter Id">R1_Bin_METABAT__11_1</Attribute>     <Attribute attribute_name="assembly quality">Many fragments with little to no review of assembly other than reporting of standard assembly statistics</Attribute>     <Attribute attribute_name="assembly software">MEGAHIT v1.2.9</Attribute>     <Attribute attribute_name="binning parameters">anvi'o 6.2 default parameters, DASTOOL with --search-engine diamond</Attribute>     <Attribute attribute_name="binning software">CONCOCT, MAXBIN2,METABAT2, refined with dastool and manually with anvi'o 6.2</Attribute>     <Attribute attribute_name="collection_date" harmonized_name="collection_date" display_name="collection date">2018-10-15</Attribute>     <Attribute attribute_name="completeness score">69.33</Attribute>     <Attribute attribute_name="completeness software">checkM v1.1.3</Attribute>     <Attribute attribute_name="contamination score">0</Attribute>     <Attribute attribute_name="environment (biome)" harmonized_name="env_broad_scale" display_name="broad-scale environmental context">marine biome [ENVO:00000447]</Attribute>     <Attribute attribute_name="environment (feature)" harmonized_name="env_local_scale" display_name="local-scale environmental context">microbial feature [ENVO:00002150]</Attribute>     <Attribute attribute_name="environment (material)" harmonized_name="env_medium" display_name="environmental medium">Macroalgae</Attribute>     <Attribute attribute_name="geographic location (country and/or sea)" harmonized_name="geo_loc_name" display_name="geographic location">China</Attribute>     <Attribute attribute_name="geographic location (latitude)">37.562</Attribute>     <Attribute attribute_name="geographic location (longitude)">122.121</Attribute>     <Attribute attribute_name="investigation type" harmonized_name="investigation_type" display_name="investigation type">metagenome-assembled genome</Attribute>     <Attribute attribute_name="isolation_source" harmonized_name="isolation_source" display_name="isolation source">Mariane macroalgea Grateloupia sp.</Attribute>     <Attribute attribute_name="metagenomic source">marine metagenome</Attribute>     <Attribute attribute_name="project name" harmonized_name="project_name" display_name="project name">Metagenomes of marine macroalgae, surrounding seawater and surrounding sediments</Attribute>     <Attribute attribute_name="sample derived from">ERS10980526</Attribute>     <Attribute attribute_name="sample name" harmonized_name="sample_name" display_name="sample name">R1_Bin_METABAT__11_1</Attribute>     <Attribute attribute_name="sequencing method">Illumina Nova-PE150</Attribute>     <Attribute attribute_name="taxonomic identity marker">multi-marker approach (GTDB-tk v1.03, GTDB v95)</Attribute>   </Attributes>   <Status status="live" when="2023-01-20T08:10:06.213"/> </BioSample> </BioSampleSet>
