SAMEA14083618 ERS11686752 Klebsiella aerogenes This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1578633 of study ERP016813. EBI Generic Generic.1.0 ERC000047 2023-01-03 2023-01-03 SAMEA14083618 EBI European Bioinformatics Institute 2023-01-03T00:33:38Z 2023-01-03T00:33:38Z public ERR1578633_bin.44_CONCOCT_v1.1_MAG Many fragments with little to no review of assembly other than reporting of standard assembly statistics metaspadesv3.10.0 Default CONCOCT v1.1 EMG broker account, EMBL-EBI 2013-01-01 100 CheckM 0.04 Host-associated Human Digestive system China 55.676098 12.568337 metagenome-assembled genome human gut metagenome human gut metagenome Coronary heart disease (CHD) is top risk factor for health in modern society, causing high mortality rate each year. However, there is no reliable way for early diagnosis and prevention of CHD so far. So study the mechanism of CHD and development of novel biomarkers is urgently needed. In this study, metabolomics and metagenomics technology are applied to discover new biomarkers from plasma and urine of 59 CHD patients and 43 healthy controls and trace their origin. We identify GlcNAc-6-P which has good diagnostic capability and can be used as potential biomarkers for CHD, together with mannitol and 15 plasma cholines. These identified metabolites show significant correlations with clinical biochemical indexes. Meanwhile, GlcNAc-6-P and mannitol are potential metabolites originated from intestinal microbiota. Association analysis on species and function levels between intestinal microbes and metabolites suggest a close correlation between Clostridium sp. HGF2 and GlcNAc-6-P, Clostridium sp. HGF2, Streptococcus sp. M143, Streptococcus sp. M334 and mannitol. These suggest the metabolic abnormality is significant and gut microbiota dysbiosis happens in CHD patients. SAMEA4378242 ERR1578633_bin.44_CONCOCT_v1.1_MAG Klebsiella aerogenes Illumina HiSeq 2000 multi-marker approach