[2023-06-28 13:30:21,888] [INFO] DFAST_QC pipeline started.
[2023-06-28 13:30:21,890] [INFO] DFAST_QC version: 0.5.7
[2023-06-28 13:30:21,890] [INFO] DQC Reference Directory: /var/lib/cwl/stg3a2ad22d-2483-4b3d-ad95-56def8a89c4f/dqc_reference
[2023-06-28 13:30:23,114] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-28 13:30:23,115] [INFO] Task started: Prodigal
[2023-06-28 13:30:23,115] [INFO] Running command: gunzip -c /var/lib/cwl/stgf87809b0-9204-479e-8eb1-9cbb0cea87e8/GCA_944321515.1_BRZ_HB_bin53_genomic.fna.gz | prodigal -d GCA_944321515.1_BRZ_HB_bin53_genomic.fna/cds.fna -a GCA_944321515.1_BRZ_HB_bin53_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-28 13:30:28,804] [INFO] Task succeeded: Prodigal
[2023-06-28 13:30:28,805] [INFO] Task started: HMMsearch
[2023-06-28 13:30:28,805] [INFO] Running command: hmmsearch --tblout GCA_944321515.1_BRZ_HB_bin53_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg3a2ad22d-2483-4b3d-ad95-56def8a89c4f/dqc_reference/reference_markers.hmm GCA_944321515.1_BRZ_HB_bin53_genomic.fna/protein.faa > /dev/null
[2023-06-28 13:30:28,972] [INFO] Task succeeded: HMMsearch
[2023-06-28 13:30:28,974] [INFO] Found 6/6 markers.
[2023-06-28 13:30:28,994] [INFO] Query marker FASTA was written to GCA_944321515.1_BRZ_HB_bin53_genomic.fna/markers.fasta
[2023-06-28 13:30:28,994] [INFO] Task started: Blastn
[2023-06-28 13:30:28,994] [INFO] Running command: blastn -query GCA_944321515.1_BRZ_HB_bin53_genomic.fna/markers.fasta -db /var/lib/cwl/stg3a2ad22d-2483-4b3d-ad95-56def8a89c4f/dqc_reference/reference_markers.fasta -out GCA_944321515.1_BRZ_HB_bin53_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-28 13:30:29,663] [INFO] Task succeeded: Blastn
[2023-06-28 13:30:29,667] [INFO] Selected 13 target genomes.
[2023-06-28 13:30:29,667] [INFO] Target genome list was writen to GCA_944321515.1_BRZ_HB_bin53_genomic.fna/target_genomes.txt
[2023-06-28 13:30:29,668] [INFO] Task started: fastANI
[2023-06-28 13:30:29,668] [INFO] Running command: fastANI --query /var/lib/cwl/stgf87809b0-9204-479e-8eb1-9cbb0cea87e8/GCA_944321515.1_BRZ_HB_bin53_genomic.fna.gz --refList GCA_944321515.1_BRZ_HB_bin53_genomic.fna/target_genomes.txt --output GCA_944321515.1_BRZ_HB_bin53_genomic.fna/fastani_result.tsv --threads 1
[2023-06-28 13:30:34,867] [INFO] Task succeeded: fastANI
[2023-06-28 13:30:34,868] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg3a2ad22d-2483-4b3d-ad95-56def8a89c4f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-28 13:30:34,868] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg3a2ad22d-2483-4b3d-ad95-56def8a89c4f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-28 13:30:34,880] [INFO] Found 13 fastANI hits (0 hits with ANI > threshold)
[2023-06-28 13:30:34,880] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2023-06-28 13:30:34,880] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Thermophilibacter mediterraneus	strain=Marseille-P3256	GCA_900119385.1	1871031	1871031	type	True	84.468	456	645	95	below_threshold
Thermophilibacter provencensis	strain=Marseille-P2912	GCA_900128445.1	1852386	1852386	type	True	84.1671	470	645	95	below_threshold
Thermophilibacter immobilis	strain=LZLJ-2	GCA_015277515.1	2779519	2779519	type	True	81.6064	370	645	95	below_threshold
Olsenella intestinalis	strain=KCTC 25379	GCA_023276655.1	2930083	2930083	type	True	80.261	276	645	95	below_threshold
Olsenella phocaeensis	strain=Marseille-P2936	GCA_900120385.1	1852385	1852385	type	True	79.9109	255	645	95	below_threshold
Parolsenella massiliensis	strain=Marseille-P3237	GCA_900143685.1	1871022	1871022	type	True	79.8856	251	645	95	below_threshold
Enorma burkinafasonensis	strain=Marseille-P9525	GCA_902150035.1	2590867	2590867	type	True	79.5621	225	645	95	below_threshold
Olsenella uli	strain=DSM 7084	GCA_000143845.1	133926	133926	suspected-type	True	79.4526	228	645	95	below_threshold
Olsenella urininfantis	strain=Marseille-P3197	GCA_900155635.1	1871033	1871033	type	True	79.4362	217	645	95	below_threshold
Olsenella uli	strain=DSM 7084	GCA_001437585.1	133926	133926	suspected-type	True	79.376	231	645	95	below_threshold
Enorma timonensis	strain=GD5	GCA_000321165.1	1232436	1232436	type	True	79.3624	219	645	95	below_threshold
Olsenella massiliensis	strain=SIT9	GCA_001457795.1	1622075	1622075	type	True	79.0046	237	645	95	below_threshold
Collinsella phocaeensis	strain=Marseille-P3245	GCA_900119895.1	1871016	1871016	type	True	78.7264	190	645	95	below_threshold
--------------------------------------------------------------------------------
[2023-06-28 13:30:34,882] [INFO] DFAST Taxonomy check result was written to GCA_944321515.1_BRZ_HB_bin53_genomic.fna/tc_result.tsv
[2023-06-28 13:30:34,883] [INFO] ===== Taxonomy check completed =====
[2023-06-28 13:30:34,883] [INFO] ===== Start completeness check using CheckM =====
[2023-06-28 13:30:34,883] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg3a2ad22d-2483-4b3d-ad95-56def8a89c4f/dqc_reference/checkm_data
[2023-06-28 13:30:34,884] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-28 13:30:34,906] [INFO] Task started: CheckM
[2023-06-28 13:30:34,906] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_944321515.1_BRZ_HB_bin53_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_944321515.1_BRZ_HB_bin53_genomic.fna/checkm_input GCA_944321515.1_BRZ_HB_bin53_genomic.fna/checkm_result
[2023-06-28 13:30:56,457] [INFO] Task succeeded: CheckM
[2023-06-28 13:30:56,458] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-28 13:30:56,473] [INFO] ===== Completeness check finished =====
[2023-06-28 13:30:56,473] [INFO] ===== Start GTDB Search =====
[2023-06-28 13:30:56,473] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_944321515.1_BRZ_HB_bin53_genomic.fna/markers.fasta)
[2023-06-28 13:30:56,474] [INFO] Task started: Blastn
[2023-06-28 13:30:56,474] [INFO] Running command: blastn -query GCA_944321515.1_BRZ_HB_bin53_genomic.fna/markers.fasta -db /var/lib/cwl/stg3a2ad22d-2483-4b3d-ad95-56def8a89c4f/dqc_reference/reference_markers_gtdb.fasta -out GCA_944321515.1_BRZ_HB_bin53_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-28 13:30:57,656] [INFO] Task succeeded: Blastn
[2023-06-28 13:30:57,660] [INFO] Selected 13 target genomes.
[2023-06-28 13:30:57,660] [INFO] Target genome list was writen to GCA_944321515.1_BRZ_HB_bin53_genomic.fna/target_genomes_gtdb.txt
[2023-06-28 13:30:57,662] [INFO] Task started: fastANI
[2023-06-28 13:30:57,662] [INFO] Running command: fastANI --query /var/lib/cwl/stgf87809b0-9204-479e-8eb1-9cbb0cea87e8/GCA_944321515.1_BRZ_HB_bin53_genomic.fna.gz --refList GCA_944321515.1_BRZ_HB_bin53_genomic.fna/target_genomes_gtdb.txt --output GCA_944321515.1_BRZ_HB_bin53_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-28 13:31:03,917] [INFO] Task succeeded: fastANI
[2023-06-28 13:31:03,929] [INFO] Found 13 fastANI hits (0 hits with ANI > circumscription radius)
[2023-06-28 13:31:03,929] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_016900315.1	s__Thermophilibacter profusa_A	88.4185	497	645	d__Bacteria;p__Actinobacteriota;c__Coriobacteriia;o__Coriobacteriales;f__Atopobiaceae;g__Thermophilibacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002160255.1	s__Thermophilibacter avistercoris	86.1353	493	645	d__Bacteria;p__Actinobacteriota;c__Coriobacteriia;o__Coriobacteriales;f__Atopobiaceae;g__Thermophilibacter	95.0	97.79	97.22	0.93	0.92	5	-
GCF_016899935.1	s__Thermophilibacter uli_B	84.68	429	645	d__Bacteria;p__Actinobacteriota;c__Coriobacteriia;o__Coriobacteriales;f__Atopobiaceae;g__Thermophilibacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002159495.1	s__Thermophilibacter sp002159495	84.6599	457	645	d__Bacteria;p__Actinobacteriota;c__Coriobacteriia;o__Coriobacteriales;f__Atopobiaceae;g__Thermophilibacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_012027725.1	s__Thermophilibacter sp012027725	84.6372	458	645	d__Bacteria;p__Actinobacteriota;c__Coriobacteriia;o__Coriobacteriales;f__Atopobiaceae;g__Thermophilibacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002159625.1	s__Thermophilibacter avicola	84.6205	451	645	d__Bacteria;p__Actinobacteriota;c__Coriobacteriia;o__Coriobacteriales;f__Atopobiaceae;g__Thermophilibacter	95.0	97.98	97.98	0.90	0.90	2	-
GCF_014982725.1	s__Thermophilibacter sp014982725	84.5364	417	645	d__Bacteria;p__Actinobacteriota;c__Coriobacteriia;o__Coriobacteriales;f__Atopobiaceae;g__Thermophilibacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002159735.1	s__Thermophilibacter sp002159735	84.4872	417	645	d__Bacteria;p__Actinobacteriota;c__Coriobacteriia;o__Coriobacteriales;f__Atopobiaceae;g__Thermophilibacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900119385.1	s__Thermophilibacter mediterraneus	84.4829	454	645	d__Bacteria;p__Actinobacteriota;c__Coriobacteriia;o__Coriobacteriales;f__Atopobiaceae;g__Thermophilibacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002159535.1	s__Thermophilibacter sp002159535	84.4499	436	645	d__Bacteria;p__Actinobacteriota;c__Coriobacteriia;o__Coriobacteriales;f__Atopobiaceae;g__Thermophilibacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900119915.1	s__Thermophilibacter timonensis	84.2459	422	645	d__Bacteria;p__Actinobacteriota;c__Coriobacteriia;o__Coriobacteriales;f__Atopobiaceae;g__Thermophilibacter	95.0	98.93	98.03	0.94	0.92	4	-
GCF_900128445.1	s__Thermophilibacter provencensis	84.1264	472	645	d__Bacteria;p__Actinobacteriota;c__Coriobacteriia;o__Coriobacteriales;f__Atopobiaceae;g__Thermophilibacter	95.0	96.98	96.17	0.87	0.80	3	-
GCA_002305805.1	s__Thermophilibacter excrementavium	84.0005	404	645	d__Bacteria;p__Actinobacteriota;c__Coriobacteriia;o__Coriobacteriales;f__Atopobiaceae;g__Thermophilibacter	95.0	97.86	97.86	0.86	0.86	2	-
--------------------------------------------------------------------------------
[2023-06-28 13:31:03,931] [INFO] GTDB search result was written to GCA_944321515.1_BRZ_HB_bin53_genomic.fna/result_gtdb.tsv
[2023-06-28 13:31:03,932] [INFO] ===== GTDB Search completed =====
[2023-06-28 13:31:03,936] [INFO] DFAST_QC result json was written to GCA_944321515.1_BRZ_HB_bin53_genomic.fna/dqc_result.json
[2023-06-28 13:31:03,936] [INFO] DFAST_QC completed!
[2023-06-28 13:31:03,936] [INFO] Total running time: 0h0m42s