<?xml version="1.0" ?>
<BioSampleSet><BioSample access="public" publication_date="2022-07-28T00:00:00.000" last_update="2022-08-01T23:14:00.000" submission_date="2022-07-29T08:29:24.316" id="30039762" accession="SAMEA110426409">   <Ids>     <Id db="BioSample" is_primary="1">SAMEA110426409</Id>     <Id db="SRA">ERS12524335</Id>   </Ids>   <Description>     <Title>Metagenome-assembled genome: SRR15725430_bin.6_metaWRAP_v1.3_MAG</Title>     <Organism taxonomy_id="747375" taxonomy_name="uncultured Solobacterium sp.">       <OrganismName>uncultured Solobacterium sp.</OrganismName>     </Organism>     <Comment>       <Paragraph>This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR15725430 of study SRP335768.</Paragraph>     </Comment>   </Description>   <Owner>     <Name>EBI</Name>   </Owner>   <Models>     <Model>Generic</Model>   </Models>   <Package display_name="Generic">Generic.1.0</Package>   <Attributes>     <Attribute attribute_name="ENA-CHECKLIST">ERC000047</Attribute>     <Attribute attribute_name="ENA-FIRST-PUBLIC">2022-07-28</Attribute>     <Attribute attribute_name="ENA-LAST-UPDATE">2022-07-28</Attribute>     <Attribute attribute_name="External Id">SAMEA110426409</Attribute>     <Attribute attribute_name="INSDC center alias">EMG</Attribute>     <Attribute attribute_name="INSDC center name">EMG</Attribute>     <Attribute attribute_name="INSDC first public">2022-07-28T16:25:48Z</Attribute>     <Attribute attribute_name="INSDC last update">2022-07-28T16:25:48Z</Attribute>     <Attribute attribute_name="INSDC status">public</Attribute>     <Attribute attribute_name="Submitter Id">SRR15725430_bin.6_metaWRAP_v1.3_MAG</Attribute>     <Attribute attribute_name="assembly quality">Many fragments with little to no review of assembly other than reporting of standard assembly statistics</Attribute>     <Attribute attribute_name="assembly software">metaSPAdes v3.15.3</Attribute>     <Attribute attribute_name="binning parameters">MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.</Attribute>     <Attribute attribute_name="binning software">metaWRAP v1.3</Attribute>     <Attribute attribute_name="broad-scale environmental context" harmonized_name="env_broad_scale" display_name="broad-scale environmental context">Pig digestive system</Attribute>     <Attribute attribute_name="broker name">EMG broker account, EMBL-EBI</Attribute>     <Attribute attribute_name="collection date" harmonized_name="collection_date" display_name="collection date">not provided</Attribute>     <Attribute attribute_name="completeness score">89.62</Attribute>     <Attribute attribute_name="completeness software">CheckM</Attribute>     <Attribute attribute_name="contamination score">0.0</Attribute>     <Attribute attribute_name="environmental medium" harmonized_name="env_medium" display_name="environmental medium">Feces</Attribute>     <Attribute attribute_name="geographic location (country and/or sea)" harmonized_name="geo_loc_name" display_name="geographic location">China</Attribute>     <Attribute attribute_name="geographic location (latitude)">29.12</Attribute>     <Attribute attribute_name="geographic location (longitude)">119.64</Attribute>     <Attribute attribute_name="investigation type" harmonized_name="investigation_type" display_name="investigation type">metagenome-assembled genome</Attribute>     <Attribute attribute_name="isolation_source" harmonized_name="isolation_source" display_name="isolation source">pig gut metagenome</Attribute>     <Attribute attribute_name="local environmental context">Intestine</Attribute>     <Attribute attribute_name="metagenomic source">pig gut metagenome</Attribute>     <Attribute attribute_name="project name" harmonized_name="project_name" display_name="project name">Lipid accumulation and high oxidative stress has become a serious health and economic problem in pig production. Fatness traits are very important in pig production since they are closely related to meat quality. Gut microbiome is generally recognized as a factor in fat deposition. However, the linkage of gut microbiota with fat accumulation in pigs remains elusive. To investigate the association among gut microbiome, lipogenic characteristics, and oxidative stress in Jinhua pigs, we selected 5 high-fat pigs and 5 low-fat pigs from 60 250-day-old Jinhua pigs in the present study and collected colon contents for metagenomic analysis, serum samples for the oxidative stress assay, and liver and abdominal fat tissues for RT-qPCR analysis.Collectively, this study would give a deep insight into how gut microbiome influence fat deposition in Jinhua pigs and would give a hint for improving growth performance and fatness traits by manipulating gut microbiome.</Attribute>     <Attribute attribute_name="sample derived from">SAMN21220124</Attribute>     <Attribute attribute_name="sample name" harmonized_name="sample_name" display_name="sample name">SRR15725430_bin.6_metaWRAP_v1.3_MAG</Attribute>     <Attribute attribute_name="sequencing method">Illumina HiSeq 2500</Attribute>     <Attribute attribute_name="taxonomic classification">The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Erysipelotrichia;o__Erysipelotrichales;f__Erysipelotrichaceae;g__Solobacterium;s__</Attribute>     <Attribute attribute_name="taxonomic identity marker">multi-marker approach</Attribute>   </Attributes>   <Status status="live" when="2022-07-30T08:22:23.957"/> </BioSample> </BioSampleSet>
