[2023-06-07 20:25:39,756] [INFO] DFAST_QC pipeline started.
[2023-06-07 20:25:39,760] [INFO] DFAST_QC version: 0.5.7
[2023-06-07 20:25:39,760] [INFO] DQC Reference Directory: /var/lib/cwl/stge5583966-ab20-4b4a-88e6-2786f438a8ec/dqc_reference
[2023-06-07 20:25:41,023] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-07 20:25:41,024] [INFO] Task started: Prodigal
[2023-06-07 20:25:41,024] [INFO] Running command: gunzip -c /var/lib/cwl/stg0930b90c-d6f5-4167-8a91-40a92819b168/GCA_945981925.1_SRR15725434_bin.11_metaWRAP_v1.3_MAG_genomic.fna.gz | prodigal -d GCA_945981925.1_SRR15725434_bin.11_metaWRAP_v1.3_MAG_genomic.fna/cds.fna -a GCA_945981925.1_SRR15725434_bin.11_metaWRAP_v1.3_MAG_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-07 20:25:43,063] [INFO] Task succeeded: Prodigal
[2023-06-07 20:25:43,064] [INFO] Task started: HMMsearch
[2023-06-07 20:25:43,064] [INFO] Running command: hmmsearch --tblout GCA_945981925.1_SRR15725434_bin.11_metaWRAP_v1.3_MAG_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stge5583966-ab20-4b4a-88e6-2786f438a8ec/dqc_reference/reference_markers.hmm GCA_945981925.1_SRR15725434_bin.11_metaWRAP_v1.3_MAG_genomic.fna/protein.faa > /dev/null
[2023-06-07 20:25:43,284] [INFO] Task succeeded: HMMsearch
[2023-06-07 20:25:43,285] [WARNING] Found 2/6 markers. [/var/lib/cwl/stg0930b90c-d6f5-4167-8a91-40a92819b168/GCA_945981925.1_SRR15725434_bin.11_metaWRAP_v1.3_MAG_genomic.fna.gz]
[2023-06-07 20:25:43,298] [INFO] Query marker FASTA was written to GCA_945981925.1_SRR15725434_bin.11_metaWRAP_v1.3_MAG_genomic.fna/markers.fasta
[2023-06-07 20:25:43,298] [INFO] Task started: Blastn
[2023-06-07 20:25:43,298] [INFO] Running command: blastn -query GCA_945981925.1_SRR15725434_bin.11_metaWRAP_v1.3_MAG_genomic.fna/markers.fasta -db /var/lib/cwl/stge5583966-ab20-4b4a-88e6-2786f438a8ec/dqc_reference/reference_markers.fasta -out GCA_945981925.1_SRR15725434_bin.11_metaWRAP_v1.3_MAG_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-07 20:25:43,982] [INFO] Task succeeded: Blastn
[2023-06-07 20:25:43,992] [INFO] Selected 6 target genomes.
[2023-06-07 20:25:43,992] [INFO] Target genome list was writen to GCA_945981925.1_SRR15725434_bin.11_metaWRAP_v1.3_MAG_genomic.fna/target_genomes.txt
[2023-06-07 20:25:43,995] [INFO] Task started: fastANI
[2023-06-07 20:25:43,996] [INFO] Running command: fastANI --query /var/lib/cwl/stg0930b90c-d6f5-4167-8a91-40a92819b168/GCA_945981925.1_SRR15725434_bin.11_metaWRAP_v1.3_MAG_genomic.fna.gz --refList GCA_945981925.1_SRR15725434_bin.11_metaWRAP_v1.3_MAG_genomic.fna/target_genomes.txt --output GCA_945981925.1_SRR15725434_bin.11_metaWRAP_v1.3_MAG_genomic.fna/fastani_result.tsv --threads 1
[2023-06-07 20:25:47,148] [INFO] Task succeeded: fastANI
[2023-06-07 20:25:47,148] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stge5583966-ab20-4b4a-88e6-2786f438a8ec/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-07 20:25:47,149] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stge5583966-ab20-4b4a-88e6-2786f438a8ec/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-07 20:25:47,151] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-07 20:25:47,151] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-07 20:25:47,151] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-07 20:25:47,153] [INFO] DFAST Taxonomy check result was written to GCA_945981925.1_SRR15725434_bin.11_metaWRAP_v1.3_MAG_genomic.fna/tc_result.tsv
[2023-06-07 20:25:47,154] [INFO] ===== Taxonomy check completed =====
[2023-06-07 20:25:47,154] [INFO] ===== Start completeness check using CheckM =====
[2023-06-07 20:25:47,155] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stge5583966-ab20-4b4a-88e6-2786f438a8ec/dqc_reference/checkm_data
[2023-06-07 20:25:47,159] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-07 20:25:47,173] [INFO] Task started: CheckM
[2023-06-07 20:25:47,173] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_945981925.1_SRR15725434_bin.11_metaWRAP_v1.3_MAG_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_945981925.1_SRR15725434_bin.11_metaWRAP_v1.3_MAG_genomic.fna/checkm_input GCA_945981925.1_SRR15725434_bin.11_metaWRAP_v1.3_MAG_genomic.fna/checkm_result
[2023-06-07 20:26:01,780] [INFO] Task succeeded: CheckM
[2023-06-07 20:26:01,781] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 56.48%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-07 20:26:01,796] [INFO] ===== Completeness check finished =====
[2023-06-07 20:26:01,797] [INFO] ===== Start GTDB Search =====
[2023-06-07 20:26:01,797] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_945981925.1_SRR15725434_bin.11_metaWRAP_v1.3_MAG_genomic.fna/markers.fasta)
[2023-06-07 20:26:01,798] [INFO] Task started: Blastn
[2023-06-07 20:26:01,798] [INFO] Running command: blastn -query GCA_945981925.1_SRR15725434_bin.11_metaWRAP_v1.3_MAG_genomic.fna/markers.fasta -db /var/lib/cwl/stge5583966-ab20-4b4a-88e6-2786f438a8ec/dqc_reference/reference_markers_gtdb.fasta -out GCA_945981925.1_SRR15725434_bin.11_metaWRAP_v1.3_MAG_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-07 20:26:02,518] [INFO] Task succeeded: Blastn
[2023-06-07 20:26:02,522] [INFO] Selected 12 target genomes.
[2023-06-07 20:26:02,523] [INFO] Target genome list was writen to GCA_945981925.1_SRR15725434_bin.11_metaWRAP_v1.3_MAG_genomic.fna/target_genomes_gtdb.txt
[2023-06-07 20:26:02,529] [INFO] Task started: fastANI
[2023-06-07 20:26:02,529] [INFO] Running command: fastANI --query /var/lib/cwl/stg0930b90c-d6f5-4167-8a91-40a92819b168/GCA_945981925.1_SRR15725434_bin.11_metaWRAP_v1.3_MAG_genomic.fna.gz --refList GCA_945981925.1_SRR15725434_bin.11_metaWRAP_v1.3_MAG_genomic.fna/target_genomes_gtdb.txt --output GCA_945981925.1_SRR15725434_bin.11_metaWRAP_v1.3_MAG_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-07 20:26:06,478] [INFO] Task succeeded: fastANI
[2023-06-07 20:26:06,484] [INFO] Found 2 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-07 20:26:06,484] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_900542285.1	s__PeH17 sp900542285	98.4785	206	214	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Christensenellales;f__CAG-138;g__PeH17	95.0	98.90	98.16	0.94	0.91	7	conclusive
GCA_004556165.1	s__PeH17 sp004556165	78.7308	57	214	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Christensenellales;f__CAG-138;g__PeH17	95.0	98.62	98.21	0.87	0.80	6	-
--------------------------------------------------------------------------------
[2023-06-07 20:26:06,487] [INFO] GTDB search result was written to GCA_945981925.1_SRR15725434_bin.11_metaWRAP_v1.3_MAG_genomic.fna/result_gtdb.tsv
[2023-06-07 20:26:06,488] [INFO] ===== GTDB Search completed =====
[2023-06-07 20:26:06,490] [INFO] DFAST_QC result json was written to GCA_945981925.1_SRR15725434_bin.11_metaWRAP_v1.3_MAG_genomic.fna/dqc_result.json
[2023-06-07 20:26:06,490] [INFO] DFAST_QC completed!
[2023-06-07 20:26:06,490] [INFO] Total running time: 0h0m27s