[2023-06-07 22:39:38,191] [INFO] DFAST_QC pipeline started.
[2023-06-07 22:39:38,193] [INFO] DFAST_QC version: 0.5.7
[2023-06-07 22:39:38,194] [INFO] DQC Reference Directory: /var/lib/cwl/stgb6e5c7b7-bd90-432e-ba37-ddecbff1131e/dqc_reference
[2023-06-07 22:39:39,666] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-07 22:39:39,667] [INFO] Task started: Prodigal
[2023-06-07 22:39:39,668] [INFO] Running command: gunzip -c /var/lib/cwl/stg2fc2cbea-6583-4743-b761-056a316da3e0/GCA_947003565.1_SRR19715303_bin.10_metawrap_v1.3_MAG_genomic.fna.gz | prodigal -d GCA_947003565.1_SRR19715303_bin.10_metawrap_v1.3_MAG_genomic.fna/cds.fna -a GCA_947003565.1_SRR19715303_bin.10_metawrap_v1.3_MAG_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-07 22:39:55,912] [INFO] Task succeeded: Prodigal
[2023-06-07 22:39:55,913] [INFO] Task started: HMMsearch
[2023-06-07 22:39:55,913] [INFO] Running command: hmmsearch --tblout GCA_947003565.1_SRR19715303_bin.10_metawrap_v1.3_MAG_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgb6e5c7b7-bd90-432e-ba37-ddecbff1131e/dqc_reference/reference_markers.hmm GCA_947003565.1_SRR19715303_bin.10_metawrap_v1.3_MAG_genomic.fna/protein.faa > /dev/null
[2023-06-07 22:39:56,165] [INFO] Task succeeded: HMMsearch
[2023-06-07 22:39:56,167] [INFO] Found 6/6 markers.
[2023-06-07 22:39:56,206] [INFO] Query marker FASTA was written to GCA_947003565.1_SRR19715303_bin.10_metawrap_v1.3_MAG_genomic.fna/markers.fasta
[2023-06-07 22:39:56,207] [INFO] Task started: Blastn
[2023-06-07 22:39:56,207] [INFO] Running command: blastn -query GCA_947003565.1_SRR19715303_bin.10_metawrap_v1.3_MAG_genomic.fna/markers.fasta -db /var/lib/cwl/stgb6e5c7b7-bd90-432e-ba37-ddecbff1131e/dqc_reference/reference_markers.fasta -out GCA_947003565.1_SRR19715303_bin.10_metawrap_v1.3_MAG_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-07 22:39:56,793] [INFO] Task succeeded: Blastn
[2023-06-07 22:39:56,798] [INFO] Selected 8 target genomes.
[2023-06-07 22:39:56,799] [INFO] Target genome list was writen to GCA_947003565.1_SRR19715303_bin.10_metawrap_v1.3_MAG_genomic.fna/target_genomes.txt
[2023-06-07 22:39:56,800] [INFO] Task started: fastANI
[2023-06-07 22:39:56,800] [INFO] Running command: fastANI --query /var/lib/cwl/stg2fc2cbea-6583-4743-b761-056a316da3e0/GCA_947003565.1_SRR19715303_bin.10_metawrap_v1.3_MAG_genomic.fna.gz --refList GCA_947003565.1_SRR19715303_bin.10_metawrap_v1.3_MAG_genomic.fna/target_genomes.txt --output GCA_947003565.1_SRR19715303_bin.10_metawrap_v1.3_MAG_genomic.fna/fastani_result.tsv --threads 1
[2023-06-07 22:40:01,995] [INFO] Task succeeded: fastANI
[2023-06-07 22:40:01,995] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgb6e5c7b7-bd90-432e-ba37-ddecbff1131e/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-07 22:40:01,996] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgb6e5c7b7-bd90-432e-ba37-ddecbff1131e/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-07 22:40:02,004] [INFO] Found 4 fastANI hits (3 hits with ANI > threshold)
[2023-06-07 22:40:02,004] [INFO] The taxonomy check result is classified as 'conclusive'.
[2023-06-07 22:40:02,004] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Akkermansia muciniphila	strain=DSM 22959	GCA_008000975.1	239935	239935	type	True	98.8564	816	858	95	conclusive
Akkermansia muciniphila	strain=ATCC BAA-835	GCA_000020225.1	239935	239935	type	True	98.8563	816	858	95	conclusive
Akkermansia muciniphila	strain=ATCC BAA-835	GCA_017504145.1	239935	239935	type	True	98.8246	819	858	95	conclusive
Akkermansia glycaniphila	strain=Pyt	GCA_001683795.1	1679444	1679444	type	True	77.1499	108	858	95	below_threshold
--------------------------------------------------------------------------------
[2023-06-07 22:40:02,006] [INFO] DFAST Taxonomy check result was written to GCA_947003565.1_SRR19715303_bin.10_metawrap_v1.3_MAG_genomic.fna/tc_result.tsv
[2023-06-07 22:40:02,007] [INFO] ===== Taxonomy check completed =====
[2023-06-07 22:40:02,007] [INFO] ===== Start completeness check using CheckM =====
[2023-06-07 22:40:02,007] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgb6e5c7b7-bd90-432e-ba37-ddecbff1131e/dqc_reference/checkm_data
[2023-06-07 22:40:02,009] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-07 22:40:02,036] [INFO] Task started: CheckM
[2023-06-07 22:40:02,036] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_947003565.1_SRR19715303_bin.10_metawrap_v1.3_MAG_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_947003565.1_SRR19715303_bin.10_metawrap_v1.3_MAG_genomic.fna/checkm_input GCA_947003565.1_SRR19715303_bin.10_metawrap_v1.3_MAG_genomic.fna/checkm_result
[2023-06-07 22:40:48,799] [INFO] Task succeeded: CheckM
[2023-06-07 22:40:48,801] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-07 22:40:48,822] [INFO] ===== Completeness check finished =====
[2023-06-07 22:40:48,822] [INFO] ===== Start GTDB Search =====
[2023-06-07 22:40:48,822] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_947003565.1_SRR19715303_bin.10_metawrap_v1.3_MAG_genomic.fna/markers.fasta)
[2023-06-07 22:40:48,823] [INFO] Task started: Blastn
[2023-06-07 22:40:48,823] [INFO] Running command: blastn -query GCA_947003565.1_SRR19715303_bin.10_metawrap_v1.3_MAG_genomic.fna/markers.fasta -db /var/lib/cwl/stgb6e5c7b7-bd90-432e-ba37-ddecbff1131e/dqc_reference/reference_markers_gtdb.fasta -out GCA_947003565.1_SRR19715303_bin.10_metawrap_v1.3_MAG_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-07 22:40:49,591] [INFO] Task succeeded: Blastn
[2023-06-07 22:40:49,599] [INFO] Selected 6 target genomes.
[2023-06-07 22:40:49,599] [INFO] Target genome list was writen to GCA_947003565.1_SRR19715303_bin.10_metawrap_v1.3_MAG_genomic.fna/target_genomes_gtdb.txt
[2023-06-07 22:40:49,601] [INFO] Task started: fastANI
[2023-06-07 22:40:49,601] [INFO] Running command: fastANI --query /var/lib/cwl/stg2fc2cbea-6583-4743-b761-056a316da3e0/GCA_947003565.1_SRR19715303_bin.10_metawrap_v1.3_MAG_genomic.fna.gz --refList GCA_947003565.1_SRR19715303_bin.10_metawrap_v1.3_MAG_genomic.fna/target_genomes_gtdb.txt --output GCA_947003565.1_SRR19715303_bin.10_metawrap_v1.3_MAG_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-07 22:40:53,696] [INFO] Task succeeded: fastANI
[2023-06-07 22:40:53,707] [INFO] Found 6 fastANI hits (1 hits with ANI > circumscription radius)
[2023-06-07 22:40:53,708] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_000020225.1	s__Akkermansia muciniphila	98.8563	816	858	d__Bacteria;p__Verrucomicrobiota;c__Verrucomicrobiae;o__Verrucomicrobiales;f__Akkermansiaceae;g__Akkermansia	95.0	98.03	97.15	0.94	0.85	200	conclusive
GCA_905200945.1	s__Akkermansia sp905200945	94.5835	795	858	d__Bacteria;p__Verrucomicrobiota;c__Verrucomicrobiae;o__Verrucomicrobiales;f__Akkermansiaceae;g__Akkermansia	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900184965.1	s__Akkermansia muciniphila_A	93.8465	750	858	d__Bacteria;p__Verrucomicrobiota;c__Verrucomicrobiae;o__Verrucomicrobiales;f__Akkermansiaceae;g__Akkermansia	95.0	98.74	97.99	0.96	0.93	10	-
GCF_018847375.1	s__Akkermansia muciniphila_B	87.424	721	858	d__Bacteria;p__Verrucomicrobiota;c__Verrucomicrobiae;o__Verrucomicrobiales;f__Akkermansiaceae;g__Akkermansia	95.0	98.85	95.28	0.95	0.86	44	-
GCF_002884975.1	s__Akkermansia muciniphila_C	85.7524	730	858	d__Bacteria;p__Verrucomicrobiota;c__Verrucomicrobiae;o__Verrucomicrobiales;f__Akkermansiaceae;g__Akkermansia	95.0	98.91	98.50	0.94	0.90	5	-
GCF_018709685.1	s__Akkermansia muciniphila_E	85.6672	705	858	d__Bacteria;p__Verrucomicrobiota;c__Verrucomicrobiae;o__Verrucomicrobiales;f__Akkermansiaceae;g__Akkermansia	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-06-07 22:40:53,710] [INFO] GTDB search result was written to GCA_947003565.1_SRR19715303_bin.10_metawrap_v1.3_MAG_genomic.fna/result_gtdb.tsv
[2023-06-07 22:40:53,710] [INFO] ===== GTDB Search completed =====
[2023-06-07 22:40:53,713] [INFO] DFAST_QC result json was written to GCA_947003565.1_SRR19715303_bin.10_metawrap_v1.3_MAG_genomic.fna/dqc_result.json
[2023-06-07 22:40:53,713] [INFO] DFAST_QC completed!
[2023-06-07 22:40:53,713] [INFO] Total running time: 0h1m16s