<?xml version="1.0" ?>
<BioSampleSet><BioSample access="public" publication_date="2022-10-03T00:00:00.000" last_update="2022-10-03T19:44:32.000" submission_date="2022-10-04T08:08:17.750" id="31148446" accession="SAMEA111423407">   <Ids>     <Id db="BioSample" is_primary="1">SAMEA111423407</Id>     <Id db="SRA">ERS13517082</Id>   </Ids>   <Description>     <Title>Metagenome-assembled genome: ERR8099095_bin.24_metawrap_v1.3_MAG</Title>     <Organism taxonomy_id="244328" taxonomy_name="uncultured Clostridia bacterium">       <OrganismName>uncultured Clostridia bacterium</OrganismName>     </Organism>     <Comment>       <Paragraph>This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR8099095 of study ERP134817.</Paragraph>     </Comment>   </Description>   <Owner>     <Name>EBI</Name>   </Owner>   <Models>     <Model>Generic</Model>   </Models>   <Package display_name="Generic">Generic.1.0</Package>   <Attributes>     <Attribute attribute_name="ENA-CHECKLIST">ERC000047</Attribute>     <Attribute attribute_name="ENA-FIRST-PUBLIC">2022-10-03</Attribute>     <Attribute attribute_name="ENA-LAST-UPDATE">2022-10-03</Attribute>     <Attribute attribute_name="External Id">SAMEA111423407</Attribute>     <Attribute attribute_name="INSDC center alias">EMG</Attribute>     <Attribute attribute_name="INSDC center name">EMG</Attribute>     <Attribute attribute_name="INSDC first public">2022-10-03T12:43:10Z</Attribute>     <Attribute attribute_name="INSDC last update">2022-10-03T12:43:10Z</Attribute>     <Attribute attribute_name="INSDC status">public</Attribute>     <Attribute attribute_name="Submitter Id">ERR8099095_bin.24_metawrap_v1.3_MAG</Attribute>     <Attribute attribute_name="assembly quality">Many fragments with little to no review of assembly other than reporting of standard assembly statistics</Attribute>     <Attribute attribute_name="assembly software">metaSPAdes v3.15.3</Attribute>     <Attribute attribute_name="binning parameters">MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.</Attribute>     <Attribute attribute_name="binning software">metawrap v1.3</Attribute>     <Attribute attribute_name="broad-scale environmental context" harmonized_name="env_broad_scale" display_name="broad-scale environmental context">mouse digestive system</Attribute>     <Attribute attribute_name="broker name">EMG broker account, EMBL-EBI</Attribute>     <Attribute attribute_name="collection date" harmonized_name="collection_date" display_name="collection date">2018-03-01</Attribute>     <Attribute attribute_name="completeness score">56.7</Attribute>     <Attribute attribute_name="completeness software">CheckM</Attribute>     <Attribute attribute_name="contamination score">1.34</Attribute>     <Attribute attribute_name="environmental medium" harmonized_name="env_medium" display_name="environmental medium">feces</Attribute>     <Attribute attribute_name="geographic location (country and/or sea)" harmonized_name="geo_loc_name" display_name="geographic location">China</Attribute>     <Attribute attribute_name="geographic location (latitude)">22.3</Attribute>     <Attribute attribute_name="geographic location (longitude)">114.18</Attribute>     <Attribute attribute_name="investigation type" harmonized_name="investigation_type" display_name="investigation type">metagenome-assembled genome</Attribute>     <Attribute attribute_name="isolation_source" harmonized_name="isolation_source" display_name="isolation source">mouse gut metagenome</Attribute>     <Attribute attribute_name="local environmental context">digestive tube</Attribute>     <Attribute attribute_name="metagenomic source">mouse gut metagenome</Attribute>     <Attribute attribute_name="project name" harmonized_name="project_name" display_name="project name">We provide metagenomic sequencing data of 16 male C57BL/6 mouse gut microbiomes. To explore the potential maternal and cage mate effects at the early stage after weaning, we chose littermates of two different mother mice and housed them in different cages. Four of the mice (My*, V*, E*, S*) were littermates of one mother mouse, while the rest four (J, Ms, N, U) were littermates of another mother mouse. My* and V* were co-housed in cage 1. U and N were co-housed in cage 3. E*, S* were co-housed together with J, and Ms in cage 2. Samples were collected at the 1st, 2nd, 9th, and 10th day after weaning (weaned at 21 days after birth). Samples of the first two time points and the last two time points were pooled, respectively, and subjected independently to shotgun metagenomic sequencing by the BIGSEQ-500 platform .</Attribute>     <Attribute attribute_name="sample derived from">SAMEA12364215</Attribute>     <Attribute attribute_name="sample name" harmonized_name="sample_name" display_name="sample name">ERR8099095_bin.24_metawrap_v1.3_MAG</Attribute>     <Attribute attribute_name="sequencing method">BGISEQ-500</Attribute>     <Attribute attribute_name="taxonomic classification">The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__;g__;s__</Attribute>     <Attribute attribute_name="taxonomic identity marker">multi-marker approach</Attribute>   </Attributes>   <Status status="live" when="2022-10-05T08:09:05.010"/> </BioSample> </BioSampleSet>
