SAMEA111463644 ERS13557674 uncultured Butyricicoccus sp. This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR899286 of study ERP010550. EBI Generic Generic.1.0 ERC000047 2022-10-13 2022-10-13 SAMEA111463644 EMG EMG 2022-10-13T12:29:00Z 2022-10-13T12:29:00Z public ERR899286_bin.70_metawrap_v1.3_MAG Many fragments with little to no review of assembly other than reporting of standard assembly statistics metaSPAdes v3.15.3 MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters. metawrap v1.3 mouse digestive system EMG broker account, EMBL-EBI not provided 93.01 CheckM 0.34 feces not provided not provided not provided metagenome-assembled genome mouse gut metagenome digestive tube mouse gut metagenome The gut microbiome is significantly altered in inflammatory bowel diseases, but the basis of these changes is not well understood. We have combined metagenomic and metatranscriptomic profiling of the gut microbiome to assess changes to both bacterial community structure and transcriptional activity in a mouse model of colitis. Gene families involved in microbial resistance to oxidative stress, including Dps/ferritin, Fe-dependent peroxidase and glutathione S-transferase, were transcriptionally up-regulated in colitis, implicating a role for increased oxygen tension in gut microbiota modulation. Transcriptional profiling of the host gut tissue and host RNA in the gut lumen revealed a marked increase in the transcription of genes with an activated macrophage and granulocyte signature, suggesting the involvement of these cell types in influencing microbial gene expression. Down-regulation of host glycosylation genes further supports a role for inflammation-driven changes to the gut niche that may impact the microbiome. We propose that members of the bacterial community react to inflammation-associated increased oxygen tension by inducing genes involved in oxidative stress resistance. Furthermore, correlated transcriptional responses between host glycosylation and bacterial glycan utilisation support a role for altered usage of host-derived carbohydrates in colitis. SAMEA3403101 ERR899286_bin.70_metawrap_v1.3_MAG Illumina HiSeq 2000 The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Butyricicoccus;s__ multi-marker approach