[2023-06-08 12:31:46,063] [INFO] DFAST_QC pipeline started.
[2023-06-08 12:31:46,068] [INFO] DFAST_QC version: 0.5.7
[2023-06-08 12:31:46,068] [INFO] DQC Reference Directory: /var/lib/cwl/stg1eed2a31-5c21-441b-9faa-761bea1fde6b/dqc_reference
[2023-06-08 12:31:47,258] [INFO] ===== Start taxonomy check using ANI =====
[2023-06-08 12:31:47,259] [INFO] Task started: Prodigal
[2023-06-08 12:31:47,259] [INFO] Running command: gunzip -c /var/lib/cwl/stg3f6f06b1-f0a6-4290-b78b-830617ec2eaf/GCA_947445775.1_CH-Jul18-22_genomic.fna.gz | prodigal -d GCA_947445775.1_CH-Jul18-22_genomic.fna/cds.fna -a GCA_947445775.1_CH-Jul18-22_genomic.fna/protein.faa -g 11 -q > /dev/null
[2023-06-08 12:31:54,577] [INFO] Task succeeded: Prodigal
[2023-06-08 12:31:54,577] [INFO] Task started: HMMsearch
[2023-06-08 12:31:54,578] [INFO] Running command: hmmsearch --tblout GCA_947445775.1_CH-Jul18-22_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg1eed2a31-5c21-441b-9faa-761bea1fde6b/dqc_reference/reference_markers.hmm GCA_947445775.1_CH-Jul18-22_genomic.fna/protein.faa > /dev/null
[2023-06-08 12:31:54,794] [INFO] Task succeeded: HMMsearch
[2023-06-08 12:31:54,795] [INFO] Found 6/6 markers.
[2023-06-08 12:31:54,820] [INFO] Query marker FASTA was written to GCA_947445775.1_CH-Jul18-22_genomic.fna/markers.fasta
[2023-06-08 12:31:54,820] [INFO] Task started: Blastn
[2023-06-08 12:31:54,821] [INFO] Running command: blastn -query GCA_947445775.1_CH-Jul18-22_genomic.fna/markers.fasta -db /var/lib/cwl/stg1eed2a31-5c21-441b-9faa-761bea1fde6b/dqc_reference/reference_markers.fasta -out GCA_947445775.1_CH-Jul18-22_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-08 12:31:55,412] [INFO] Task succeeded: Blastn
[2023-06-08 12:31:55,418] [INFO] Selected 20 target genomes.
[2023-06-08 12:31:55,418] [INFO] Target genome list was writen to GCA_947445775.1_CH-Jul18-22_genomic.fna/target_genomes.txt
[2023-06-08 12:31:55,424] [INFO] Task started: fastANI
[2023-06-08 12:31:55,424] [INFO] Running command: fastANI --query /var/lib/cwl/stg3f6f06b1-f0a6-4290-b78b-830617ec2eaf/GCA_947445775.1_CH-Jul18-22_genomic.fna.gz --refList GCA_947445775.1_CH-Jul18-22_genomic.fna/target_genomes.txt --output GCA_947445775.1_CH-Jul18-22_genomic.fna/fastani_result.tsv --threads 1
[2023-06-08 12:32:06,373] [INFO] Task succeeded: fastANI
[2023-06-08 12:32:06,374] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg1eed2a31-5c21-441b-9faa-761bea1fde6b/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2023-06-08 12:32:06,374] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg1eed2a31-5c21-441b-9faa-761bea1fde6b/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2023-06-08 12:32:06,376] [INFO] Found 0 fastANI hits (0 hits with ANI > threshold)
[2023-06-08 12:32:06,377] [INFO] The taxonomy check result is classified as 'no_hit'.
[2023-06-08 12:32:06,377] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
--------------------------------------------------------------------------------
[2023-06-08 12:32:06,380] [INFO] DFAST Taxonomy check result was written to GCA_947445775.1_CH-Jul18-22_genomic.fna/tc_result.tsv
[2023-06-08 12:32:06,381] [INFO] ===== Taxonomy check completed =====
[2023-06-08 12:32:06,381] [INFO] ===== Start completeness check using CheckM =====
[2023-06-08 12:32:06,382] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg1eed2a31-5c21-441b-9faa-761bea1fde6b/dqc_reference/checkm_data
[2023-06-08 12:32:06,386] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2023-06-08 12:32:06,415] [INFO] Task started: CheckM
[2023-06-08 12:32:06,416] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCA_947445775.1_CH-Jul18-22_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCA_947445775.1_CH-Jul18-22_genomic.fna/checkm_input GCA_947445775.1_CH-Jul18-22_genomic.fna/checkm_result
[2023-06-08 12:32:34,596] [INFO] Task succeeded: CheckM
[2023-06-08 12:32:34,597] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 82.95%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2023-06-08 12:32:34,619] [INFO] ===== Completeness check finished =====
[2023-06-08 12:32:34,620] [INFO] ===== Start GTDB Search =====
[2023-06-08 12:32:34,620] [INFO] Query marker FASTA already exists. Will reuse it. (GCA_947445775.1_CH-Jul18-22_genomic.fna/markers.fasta)
[2023-06-08 12:32:34,620] [INFO] Task started: Blastn
[2023-06-08 12:32:34,621] [INFO] Running command: blastn -query GCA_947445775.1_CH-Jul18-22_genomic.fna/markers.fasta -db /var/lib/cwl/stg1eed2a31-5c21-441b-9faa-761bea1fde6b/dqc_reference/reference_markers_gtdb.fasta -out GCA_947445775.1_CH-Jul18-22_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2023-06-08 12:32:35,493] [INFO] Task succeeded: Blastn
[2023-06-08 12:32:35,498] [INFO] Selected 20 target genomes.
[2023-06-08 12:32:35,498] [INFO] Target genome list was writen to GCA_947445775.1_CH-Jul18-22_genomic.fna/target_genomes_gtdb.txt
[2023-06-08 12:32:35,515] [INFO] Task started: fastANI
[2023-06-08 12:32:35,515] [INFO] Running command: fastANI --query /var/lib/cwl/stg3f6f06b1-f0a6-4290-b78b-830617ec2eaf/GCA_947445775.1_CH-Jul18-22_genomic.fna.gz --refList GCA_947445775.1_CH-Jul18-22_genomic.fna/target_genomes_gtdb.txt --output GCA_947445775.1_CH-Jul18-22_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2023-06-08 12:32:47,105] [INFO] Task succeeded: fastANI
[2023-06-08 12:32:47,113] [INFO] Found 1 fastANI hits (0 hits with ANI > circumscription radius)
[2023-06-08 12:32:47,113] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_010029825.1	s__RGVZ01 sp010029825	77.2288	140	797	d__Bacteria;p__Bdellovibrionota;c__Oligoflexia;o__Oligoflexales;f__RGVZ01;g__RGVZ01	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2023-06-08 12:32:47,117] [INFO] GTDB search result was written to GCA_947445775.1_CH-Jul18-22_genomic.fna/result_gtdb.tsv
[2023-06-08 12:32:47,118] [INFO] ===== GTDB Search completed =====
[2023-06-08 12:32:47,121] [INFO] DFAST_QC result json was written to GCA_947445775.1_CH-Jul18-22_genomic.fna/dqc_result.json
[2023-06-08 12:32:47,121] [INFO] DFAST_QC completed!
[2023-06-08 12:32:47,121] [INFO] Total running time: 0h1m1s