SAMEA112250310 ERS14361173 uncultured Muribaculaceae bacterium This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR18243799 of study SRP362674. EBI Generic Generic.1.0 ERC000047 2023-01-19 2023-01-19 SAMEA112250310 EMG EMG 2023-01-19T20:22:09Z 2023-01-19T20:22:09Z public SRR18243799_bin.17_metawrap_v1.3_MAG Many fragments with little to no review of assembly other than reporting of standard assembly statistics metaSPAdes v3.15.3 MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters. metawrap v1.3 mouse digestive system EMG broker account, EMBL-EBI 2016-03-10 98.3 CheckM 0.38 feces Germany 52.62686 13.507567 metagenome-assembled genome mouse gut metagenome digestive tube mouse gut metagenome Hypertension (HTN) can lead to heart and kidney damage. The gut microbiota has been linked to HTN, although it is difficult to estimate its significance due to the variety of other features known to influence HTN. In the present study, we used germ-free (GF) and colonized (COL) littermate mice to quantify the impact of microbial colonization on organ damage in HTN.</p><p>Four-week-old male GF C57BL/6J littermates were randomized to remain GF or receive microbial colonization. HTN was induced by subcutaneous infusion with angiotensin (Ang) II (1.44mg/kg/d) and 1% NaCl in the drinking water; sham-treated mice served as control. Renal damage was exacerbated in GF mice, whereas cardiac damage was more comparable between COL and GF, suggesting that the kidney is more sensitive to microbial influence. Multivariate analysis revealed a larger effect of HTN in GF mice. Serum metabolomics demonstrated that the colonization status influences circulating metabolites relevant to HTN. Importantly, GF mice were deficient in anti-inflammatory fecal short-chain fatty acids (SCFA). Flow cytometry showed that the microbiome has an impact on the induction of anti-hypertensive myeloid-derived suppressor cells and pro-inflammatory Th17 cells in HTN. In vitro inducibility of Th17 cells was significantly higher for cells isolated from GF than conventionally raised mice.</p><p>Microbial colonization status of mice had potent effects on their phenotypic response to a hypertensive stimulus, and the kidney is a highly microbiota-susceptible target organ in HTN. The magnitude of the pathogenic response in GF mice underscores the role of the microbiome in mediating inflammation in HTN. SAMN26378637 SRR18243799_bin.17_metawrap_v1.3_MAG Illumina NovaSeq 6000 The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Bacteroidetes;c__Bacteroidia;o__Bacteroidales;f__Muribaculaceae;g__;s__ multi-marker approach