SAMEA112229495 ERS14340394 uncultured Clostridia bacterium This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR15604611 of study SRP334008. EBI Generic Generic.1.0 ERC000047 2023-01-25 2023-01-25 SAMEA112229495 EMG EMG 2023-01-25T00:20:14Z 2023-01-25T00:20:14Z public SRR15604611_bin.26_metawrap_v1.3_MAG Many fragments with little to no review of assembly other than reporting of standard assembly statistics not provided MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters. metawrap v1.3 mouse digestive system EMG broker account, EMBL-EBI 2019-11-18 94.76 CheckM 0.0 feces China 40.51 110.168 metagenome-assembled genome mouse gut metagenome digestive tube mouse gut metagenome To evaluate the role of probiotics in restoring the antibiotic-destroyed gut microbiota and efficacy of ICB therapies, we studied the effect of Lactobacillus rhamnosus Probio-M9 combined with anti-PD-1 on mouse colon cancer and the dynamic changes of gut microbiome by metagenome analyses. The results showed that the combination of Probio-M9 significantly improved tumor inhibition compared with ICB therapy alone in antibiotics treated mice, which could be attribute to the upregulated microbial diversity and increased abundance of beneficial bacteria such as Bifidobacterium pseudolongum, Parabacteroides distasonis and some species of Bacteroides after Probio-M9 supplemented. These changes of structure and composition in gut microbiota caused the alternation of microbiome function, of which the sugar degradation, vitamin and amino acid synthesis pathways were significantly enriched. Collectively, this study indicated that Probio-M9 enhanced the host's immunotherapeutic response by promoting the colonization of beneficial bacteria and improving intestinal function. SAMN20968510 SRR15604611_bin.26_metawrap_v1.3_MAG Illumina HiSeq 2500 The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Catabacteriaceae;g__;s__ multi-marker approach