[2024-01-24 13:17:34,457] [INFO] DFAST_QC pipeline started.
[2024-01-24 13:17:34,459] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 13:17:34,460] [INFO] DQC Reference Directory: /var/lib/cwl/stg55cb69df-a259-42ad-9457-d6fed92051ef/dqc_reference
[2024-01-24 13:17:35,923] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 13:17:35,924] [INFO] Task started: Prodigal
[2024-01-24 13:17:35,924] [INFO] Running command: gunzip -c /var/lib/cwl/stge77598f8-56a1-4f9c-9caf-b4f68944a96b/GCF_000019945.1_ASM1994v1_genomic.fna.gz | prodigal -d GCF_000019945.1_ASM1994v1_genomic.fna/cds.fna -a GCF_000019945.1_ASM1994v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 13:17:52,669] [INFO] Task succeeded: Prodigal
[2024-01-24 13:17:52,669] [INFO] Task started: HMMsearch
[2024-01-24 13:17:52,670] [INFO] Running command: hmmsearch --tblout GCF_000019945.1_ASM1994v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg55cb69df-a259-42ad-9457-d6fed92051ef/dqc_reference/reference_markers.hmm GCF_000019945.1_ASM1994v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 13:17:52,995] [INFO] Task succeeded: HMMsearch
[2024-01-24 13:17:52,996] [INFO] Found 6/6 markers.
[2024-01-24 13:17:53,047] [INFO] Query marker FASTA was written to GCF_000019945.1_ASM1994v1_genomic.fna/markers.fasta
[2024-01-24 13:17:53,048] [INFO] Task started: Blastn
[2024-01-24 13:17:53,048] [INFO] Running command: blastn -query GCF_000019945.1_ASM1994v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg55cb69df-a259-42ad-9457-d6fed92051ef/dqc_reference/reference_markers.fasta -out GCF_000019945.1_ASM1994v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:17:54,064] [INFO] Task succeeded: Blastn
[2024-01-24 13:17:54,068] [INFO] Selected 10 target genomes.
[2024-01-24 13:17:54,068] [INFO] Target genome list was writen to GCF_000019945.1_ASM1994v1_genomic.fna/target_genomes.txt
[2024-01-24 13:17:54,072] [INFO] Task started: fastANI
[2024-01-24 13:17:54,072] [INFO] Running command: fastANI --query /var/lib/cwl/stge77598f8-56a1-4f9c-9caf-b4f68944a96b/GCF_000019945.1_ASM1994v1_genomic.fna.gz --refList GCF_000019945.1_ASM1994v1_genomic.fna/target_genomes.txt --output GCF_000019945.1_ASM1994v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 13:18:08,634] [INFO] Task succeeded: fastANI
[2024-01-24 13:18:08,635] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg55cb69df-a259-42ad-9457-d6fed92051ef/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 13:18:08,635] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg55cb69df-a259-42ad-9457-d6fed92051ef/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 13:18:08,645] [INFO] Found 10 fastANI hits (0 hits with ANI > threshold)
[2024-01-24 13:18:08,646] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2024-01-24 13:18:08,646] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Methylorubrum podarium	strain=DSM 15083	GCA_022179745.1	200476	200476	type	True	90.5876	1088	1948	95	below_threshold
Methylorubrum rhodesianum	strain=DSM 5687	GCA_014199985.1	29427	29427	type	True	90.5651	1532	1948	95	below_threshold
Methylorubrum aminovorans	strain=NBRC 15686	GCA_022179725.1	269069	269069	type	True	89.9159	1454	1948	95	below_threshold
Methylorubrum extorquens	strain=TK 0001	GCA_900234795.1	408	408	type	True	89.6685	1478	1948	95	below_threshold
Methylorubrum zatmanii	strain=LMG 6087	GCA_014845115.1	29429	29429	type	True	87.8496	1196	1948	95	below_threshold
Methylorubrum rhodinum	strain=DSM 2163	GCA_014199935.1	29428	29428	type	True	85.9491	1221	1948	95	below_threshold
Methylorubrum salsuginis	strain=CGMCC 1.6474	GCA_900114375.1	414703	414703	type	True	85.6475	1247	1948	95	below_threshold
Methylorubrum suomiense	strain=DSM 14458	GCA_022179765.1	144191	144191	type	True	85.2701	1250	1948	95	below_threshold
Methylobacterium gregans	strain=NBRC 103626	GCA_022179245.1	374424	374424	type	True	83.3155	1020	1948	95	below_threshold
Methylobacterium gnaphalii	strain=DSM 24027	GCA_022179205.1	1010610	1010610	type	True	81.6325	841	1948	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 13:18:08,648] [INFO] DFAST Taxonomy check result was written to GCF_000019945.1_ASM1994v1_genomic.fna/tc_result.tsv
[2024-01-24 13:18:08,648] [INFO] ===== Taxonomy check completed =====
[2024-01-24 13:18:08,649] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 13:18:08,649] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg55cb69df-a259-42ad-9457-d6fed92051ef/dqc_reference/checkm_data
[2024-01-24 13:18:08,650] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 13:18:08,709] [INFO] Task started: CheckM
[2024-01-24 13:18:08,709] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_000019945.1_ASM1994v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_000019945.1_ASM1994v1_genomic.fna/checkm_input GCF_000019945.1_ASM1994v1_genomic.fna/checkm_result
[2024-01-24 13:19:04,396] [INFO] Task succeeded: CheckM
[2024-01-24 13:19:04,398] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 13:19:04,421] [INFO] ===== Completeness check finished =====
[2024-01-24 13:19:04,422] [INFO] ===== Start GTDB Search =====
[2024-01-24 13:19:04,422] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_000019945.1_ASM1994v1_genomic.fna/markers.fasta)
[2024-01-24 13:19:04,423] [INFO] Task started: Blastn
[2024-01-24 13:19:04,423] [INFO] Running command: blastn -query GCF_000019945.1_ASM1994v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg55cb69df-a259-42ad-9457-d6fed92051ef/dqc_reference/reference_markers_gtdb.fasta -out GCF_000019945.1_ASM1994v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:19:06,302] [INFO] Task succeeded: Blastn
[2024-01-24 13:19:06,307] [INFO] Selected 9 target genomes.
[2024-01-24 13:19:06,307] [INFO] Target genome list was writen to GCF_000019945.1_ASM1994v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 13:19:06,322] [INFO] Task started: fastANI
[2024-01-24 13:19:06,322] [INFO] Running command: fastANI --query /var/lib/cwl/stge77598f8-56a1-4f9c-9caf-b4f68944a96b/GCF_000019945.1_ASM1994v1_genomic.fna.gz --refList GCF_000019945.1_ASM1994v1_genomic.fna/target_genomes_gtdb.txt --output GCF_000019945.1_ASM1994v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 13:19:20,328] [INFO] Task succeeded: fastANI
[2024-01-24 13:19:20,340] [INFO] Found 9 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 13:19:20,341] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_014138645.1	s__Methylobacterium thiocyanatum	97.9092	1648	1948	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Beijerinckiaceae;g__Methylobacterium	95.0	97.87	97.76	0.88	0.86	7	conclusive
GCF_002355515.1	s__Methylobacterium populi_A	90.8923	1539	1948	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Beijerinckiaceae;g__Methylobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014199985.1	s__Methylobacterium rhodesianum	90.5744	1530	1948	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Beijerinckiaceae;g__Methylobacterium	95.0	98.84	98.11	0.88	0.84	5	-
GCF_900234795.1	s__Methylobacterium extorquens	89.6908	1476	1948	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Beijerinckiaceae;g__Methylobacterium	95.0	97.03	96.19	0.88	0.73	17	-
GCF_001542815.1	s__Methylobacterium sp001542815	89.6735	1446	1948	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Beijerinckiaceae;g__Methylobacterium	95.0	98.78	98.78	0.92	0.92	2	-
GCF_001423405.1	s__Methylobacterium sp001423405	89.5402	1406	1948	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Beijerinckiaceae;g__Methylobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003111705.1	s__Methylobacterium sp003111705	89.4565	1453	1948	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Beijerinckiaceae;g__Methylobacterium	95.0	97.28	97.28	0.88	0.88	3	-
GCF_014191355.1	s__Methylobacterium sp014191355	89.2052	1411	1948	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Beijerinckiaceae;g__Methylobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014845115.1	s__Methylobacterium zatmanii	87.8229	1200	1948	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Beijerinckiaceae;g__Methylobacterium	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 13:19:20,342] [INFO] GTDB search result was written to GCF_000019945.1_ASM1994v1_genomic.fna/result_gtdb.tsv
[2024-01-24 13:19:20,343] [INFO] ===== GTDB Search completed =====
[2024-01-24 13:19:20,346] [INFO] DFAST_QC result json was written to GCF_000019945.1_ASM1994v1_genomic.fna/dqc_result.json
[2024-01-24 13:19:20,346] [INFO] DFAST_QC completed!
[2024-01-24 13:19:20,346] [INFO] Total running time: 0h1m46s