[2024-01-24 13:45:46,546] [INFO] DFAST_QC pipeline started.
[2024-01-24 13:45:46,548] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 13:45:46,548] [INFO] DQC Reference Directory: /var/lib/cwl/stg10aa786b-3e6c-4fb8-b57b-f32009137a14/dqc_reference
[2024-01-24 13:45:47,953] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 13:45:47,954] [INFO] Task started: Prodigal
[2024-01-24 13:45:47,954] [INFO] Running command: gunzip -c /var/lib/cwl/stg691d6db8-9aa8-495f-a0ae-5a609997b0a4/GCF_000160595.1_ASM16059v1_genomic.fna.gz | prodigal -d GCF_000160595.1_ASM16059v1_genomic.fna/cds.fna -a GCF_000160595.1_ASM16059v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 13:45:51,960] [INFO] Task succeeded: Prodigal
[2024-01-24 13:45:51,961] [INFO] Task started: HMMsearch
[2024-01-24 13:45:51,961] [INFO] Running command: hmmsearch --tblout GCF_000160595.1_ASM16059v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg10aa786b-3e6c-4fb8-b57b-f32009137a14/dqc_reference/reference_markers.hmm GCF_000160595.1_ASM16059v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 13:45:52,263] [INFO] Task succeeded: HMMsearch
[2024-01-24 13:45:52,265] [INFO] Found 6/6 markers.
[2024-01-24 13:45:52,283] [INFO] Query marker FASTA was written to GCF_000160595.1_ASM16059v1_genomic.fna/markers.fasta
[2024-01-24 13:45:52,284] [INFO] Task started: Blastn
[2024-01-24 13:45:52,284] [INFO] Running command: blastn -query GCF_000160595.1_ASM16059v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg10aa786b-3e6c-4fb8-b57b-f32009137a14/dqc_reference/reference_markers.fasta -out GCF_000160595.1_ASM16059v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:45:52,876] [INFO] Task succeeded: Blastn
[2024-01-24 13:45:52,879] [INFO] Selected 12 target genomes.
[2024-01-24 13:45:52,879] [INFO] Target genome list was writen to GCF_000160595.1_ASM16059v1_genomic.fna/target_genomes.txt
[2024-01-24 13:45:52,907] [INFO] Task started: fastANI
[2024-01-24 13:45:52,908] [INFO] Running command: fastANI --query /var/lib/cwl/stg691d6db8-9aa8-495f-a0ae-5a609997b0a4/GCF_000160595.1_ASM16059v1_genomic.fna.gz --refList GCF_000160595.1_ASM16059v1_genomic.fna/target_genomes.txt --output GCF_000160595.1_ASM16059v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 13:45:57,579] [INFO] Task succeeded: fastANI
[2024-01-24 13:45:57,580] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg10aa786b-3e6c-4fb8-b57b-f32009137a14/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 13:45:57,580] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg10aa786b-3e6c-4fb8-b57b-f32009137a14/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 13:45:57,592] [INFO] Found 7 fastANI hits (7 hits with ANI > threshold)
[2024-01-24 13:45:57,592] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 13:45:57,592] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Leuconostoc mesenteroides subsp. cremoris	strain=ATCC 19254	GCA_000160595.1	33965	1245	type	True	99.9996	559	569	95	conclusive
Leuconostoc mesenteroides subsp. dextranicum	strain=NBRC 100495	GCA_007990145.1	33966	1245	type	True	98.5859	455	569	95	conclusive
Leuconostoc mesenteroides subsp. mesenteroides	strain=ATCC 8293	GCA_000014445.1	33967	1245	type	True	98.3647	482	569	95	conclusive
Leuconostoc mesenteroides subsp. mesenteroides	strain=NBRC 100496	GCA_007990165.1	33967	1245	type	True	98.3636	469	569	95	conclusive
Leuconostoc mesenteroides subsp. dextranicum	strain=DSM 20484	GCA_001047695.1	33966	1245	type	True	98.3431	483	569	95	conclusive
Leuconostoc mesenteroides	strain=NCTC12954	GCA_900461615.1	1245	1245	type	True	98.2795	483	569	95	conclusive
Leuconostoc mesenteroides subsp. jonggajibkimchii	strain=DRC1506	GCA_001886915.1	2026657	1245	type	True	98.1758	477	569	95	conclusive
--------------------------------------------------------------------------------
[2024-01-24 13:45:57,594] [INFO] DFAST Taxonomy check result was written to GCF_000160595.1_ASM16059v1_genomic.fna/tc_result.tsv
[2024-01-24 13:45:57,594] [INFO] ===== Taxonomy check completed =====
[2024-01-24 13:45:57,595] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 13:45:57,595] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg10aa786b-3e6c-4fb8-b57b-f32009137a14/dqc_reference/checkm_data
[2024-01-24 13:45:57,596] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 13:45:57,619] [INFO] Task started: CheckM
[2024-01-24 13:45:57,619] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_000160595.1_ASM16059v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_000160595.1_ASM16059v1_genomic.fna/checkm_input GCF_000160595.1_ASM16059v1_genomic.fna/checkm_result
[2024-01-24 13:46:17,167] [INFO] Task succeeded: CheckM
[2024-01-24 13:46:17,169] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 13:46:17,201] [INFO] ===== Completeness check finished =====
[2024-01-24 13:46:17,201] [INFO] ===== Start GTDB Search =====
[2024-01-24 13:46:17,202] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_000160595.1_ASM16059v1_genomic.fna/markers.fasta)
[2024-01-24 13:46:17,202] [INFO] Task started: Blastn
[2024-01-24 13:46:17,202] [INFO] Running command: blastn -query GCF_000160595.1_ASM16059v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg10aa786b-3e6c-4fb8-b57b-f32009137a14/dqc_reference/reference_markers_gtdb.fasta -out GCF_000160595.1_ASM16059v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:46:18,053] [INFO] Task succeeded: Blastn
[2024-01-24 13:46:18,057] [INFO] Selected 11 target genomes.
[2024-01-24 13:46:18,057] [INFO] Target genome list was writen to GCF_000160595.1_ASM16059v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 13:46:18,079] [INFO] Task started: fastANI
[2024-01-24 13:46:18,079] [INFO] Running command: fastANI --query /var/lib/cwl/stg691d6db8-9aa8-495f-a0ae-5a609997b0a4/GCF_000160595.1_ASM16059v1_genomic.fna.gz --refList GCF_000160595.1_ASM16059v1_genomic.fna/target_genomes_gtdb.txt --output GCF_000160595.1_ASM16059v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 13:46:22,469] [INFO] Task succeeded: fastANI
[2024-01-24 13:46:22,478] [INFO] Found 9 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 13:46:22,478] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_000014445.1	s__Leuconostoc mesenteroides	98.3237	483	569	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Leuconostoc	95.0	98.82	97.34	0.92	0.82	89	conclusive
GCF_001891125.1	s__Leuconostoc suionicum	93.7961	467	569	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Leuconostoc	95.0	99.16	97.05	0.94	0.91	7	-
GCF_009676745.1	s__Leuconostoc mesenteroides_B	90.9959	453	569	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Leuconostoc	95.0	100.00	100.00	0.99	0.99	2	-
GCF_008107645.1	s__Leuconostoc litchii	82.2456	355	569	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Leuconostoc	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014634805.1	s__Leuconostoc falkenbergense	81.6303	196	569	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Leuconostoc	95.0	99.11	96.75	0.91	0.87	34	-
GCF_000092505.1	s__Leuconostoc kimchii	80.3573	174	569	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Leuconostoc	95.0	99.55	99.53	0.95	0.95	3	-
GCF_014634745.1	s__Leuconostoc pseudomesenteroides	80.1737	214	569	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Leuconostoc	95.0	97.98	96.85	0.90	0.84	11	-
GCF_014207505.1	s__Leuconostoc carnosum	79.8513	157	569	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Leuconostoc	95.0	99.31	98.70	0.92	0.87	17	-
GCF_016908715.1	s__Leuconostoc rapi	79.2503	151	569	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Leuconostoc	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 13:46:22,504] [INFO] GTDB search result was written to GCF_000160595.1_ASM16059v1_genomic.fna/result_gtdb.tsv
[2024-01-24 13:46:22,505] [INFO] ===== GTDB Search completed =====
[2024-01-24 13:46:22,508] [INFO] DFAST_QC result json was written to GCF_000160595.1_ASM16059v1_genomic.fna/dqc_result.json
[2024-01-24 13:46:22,508] [INFO] DFAST_QC completed!
[2024-01-24 13:46:22,508] [INFO] Total running time: 0h0m36s