[2024-01-24 14:19:44,202] [INFO] DFAST_QC pipeline started.
[2024-01-24 14:19:44,204] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 14:19:44,204] [INFO] DQC Reference Directory: /var/lib/cwl/stg6aff9aa1-8462-4210-9c34-887c5baa76ff/dqc_reference
[2024-01-24 14:19:45,615] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 14:19:45,616] [INFO] Task started: Prodigal
[2024-01-24 14:19:45,616] [INFO] Running command: gunzip -c /var/lib/cwl/stg8472e23a-66e9-4599-bb83-0ee8987fea61/GCF_000178475.1_ASM17847v1_genomic.fna.gz | prodigal -d GCF_000178475.1_ASM17847v1_genomic.fna/cds.fna -a GCF_000178475.1_ASM17847v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 14:19:48,981] [INFO] Task succeeded: Prodigal
[2024-01-24 14:19:48,981] [INFO] Task started: HMMsearch
[2024-01-24 14:19:48,981] [INFO] Running command: hmmsearch --tblout GCF_000178475.1_ASM17847v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg6aff9aa1-8462-4210-9c34-887c5baa76ff/dqc_reference/reference_markers.hmm GCF_000178475.1_ASM17847v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 14:19:49,223] [INFO] Task succeeded: HMMsearch
[2024-01-24 14:19:49,225] [INFO] Found 6/6 markers.
[2024-01-24 14:19:49,243] [INFO] Query marker FASTA was written to GCF_000178475.1_ASM17847v1_genomic.fna/markers.fasta
[2024-01-24 14:19:49,244] [INFO] Task started: Blastn
[2024-01-24 14:19:49,244] [INFO] Running command: blastn -query GCF_000178475.1_ASM17847v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg6aff9aa1-8462-4210-9c34-887c5baa76ff/dqc_reference/reference_markers.fasta -out GCF_000178475.1_ASM17847v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:19:49,864] [INFO] Task succeeded: Blastn
[2024-01-24 14:19:49,867] [INFO] Selected 14 target genomes.
[2024-01-24 14:19:49,868] [INFO] Target genome list was writen to GCF_000178475.1_ASM17847v1_genomic.fna/target_genomes.txt
[2024-01-24 14:19:49,874] [INFO] Task started: fastANI
[2024-01-24 14:19:49,874] [INFO] Running command: fastANI --query /var/lib/cwl/stg8472e23a-66e9-4599-bb83-0ee8987fea61/GCF_000178475.1_ASM17847v1_genomic.fna.gz --refList GCF_000178475.1_ASM17847v1_genomic.fna/target_genomes.txt --output GCF_000178475.1_ASM17847v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 14:19:54,636] [INFO] Task succeeded: fastANI
[2024-01-24 14:19:54,637] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg6aff9aa1-8462-4210-9c34-887c5baa76ff/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 14:19:54,637] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg6aff9aa1-8462-4210-9c34-887c5baa76ff/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 14:19:54,646] [INFO] Found 10 fastANI hits (3 hits with ANI > threshold)
[2024-01-24 14:19:54,646] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 14:19:54,647] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Lactobacillus amylolyticus	strain=DSM 11664	GCA_000178475.1	83683	83683	type	True	100.0	476	477	95	conclusive
Lactobacillus amylolyticus	strain=DSM 11664	GCA_004354545.1	83683	83683	type	True	99.9973	477	477	95	conclusive
Lactobacillus amylolyticus	strain=DSM 11664	GCA_001435665.1	83683	83683	type	True	99.9564	460	477	95	conclusive
Lactobacillus hamsteri	strain=DSM 5661	GCA_001434515.1	96565	96565	type	True	80.9992	264	477	95	below_threshold
Lactobacillus kitasatonis	strain=DSM 16761	GCA_001434435.1	237446	237446	type	True	80.9341	227	477	95	below_threshold
Lactobacillus hamsteri	strain=JCM 6256	GCA_000615445.1	96565	96565	type	True	80.9291	261	477	95	below_threshold
Lactobacillus ultunensis	strain=DSM 16047	GCA_001436305.1	227945	227945	type	True	80.5167	208	477	95	below_threshold
Lactobacillus xujianguonis	strain=HT111-2	GCA_004009905.1	2495899	2495899	type	True	80.4586	225	477	95	below_threshold
Lactobacillus ultunensis	strain=DSM 16047	GCA_000159415.1	227945	227945	type	True	80.4394	223	477	95	below_threshold
Lactobacillus rodentium	strain=DSM 24759	GCA_024622425.1	947835	947835	type	True	79.1519	94	477	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 14:19:54,648] [INFO] DFAST Taxonomy check result was written to GCF_000178475.1_ASM17847v1_genomic.fna/tc_result.tsv
[2024-01-24 14:19:54,649] [INFO] ===== Taxonomy check completed =====
[2024-01-24 14:19:54,649] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 14:19:54,649] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg6aff9aa1-8462-4210-9c34-887c5baa76ff/dqc_reference/checkm_data
[2024-01-24 14:19:54,650] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 14:19:54,669] [INFO] Task started: CheckM
[2024-01-24 14:19:54,669] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_000178475.1_ASM17847v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_000178475.1_ASM17847v1_genomic.fna/checkm_input GCF_000178475.1_ASM17847v1_genomic.fna/checkm_result
[2024-01-24 14:20:13,649] [INFO] Task succeeded: CheckM
[2024-01-24 14:20:13,650] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 14:20:13,681] [INFO] ===== Completeness check finished =====
[2024-01-24 14:20:13,682] [INFO] ===== Start GTDB Search =====
[2024-01-24 14:20:13,682] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_000178475.1_ASM17847v1_genomic.fna/markers.fasta)
[2024-01-24 14:20:13,683] [INFO] Task started: Blastn
[2024-01-24 14:20:13,683] [INFO] Running command: blastn -query GCF_000178475.1_ASM17847v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg6aff9aa1-8462-4210-9c34-887c5baa76ff/dqc_reference/reference_markers_gtdb.fasta -out GCF_000178475.1_ASM17847v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:20:14,445] [INFO] Task succeeded: Blastn
[2024-01-24 14:20:14,449] [INFO] Selected 13 target genomes.
[2024-01-24 14:20:14,449] [INFO] Target genome list was writen to GCF_000178475.1_ASM17847v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 14:20:14,485] [INFO] Task started: fastANI
[2024-01-24 14:20:14,485] [INFO] Running command: fastANI --query /var/lib/cwl/stg8472e23a-66e9-4599-bb83-0ee8987fea61/GCF_000178475.1_ASM17847v1_genomic.fna.gz --refList GCF_000178475.1_ASM17847v1_genomic.fna/target_genomes_gtdb.txt --output GCF_000178475.1_ASM17847v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 14:20:19,228] [INFO] Task succeeded: fastANI
[2024-01-24 14:20:19,240] [INFO] Found 13 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 14:20:19,241] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_000178475.1	s__Lactobacillus amylolyticus	100.0	476	477	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus	95.0	99.45	98.00	0.96	0.87	8	conclusive
GCF_001434975.1	s__Lactobacillus gallinarum	81.3757	230	477	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus	95.0	98.30	97.84	0.88	0.84	17	-
GCF_002706375.1	s__Lactobacillus amylovorus	81.3639	236	477	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus	95.0	97.43	96.81	0.87	0.78	24	-
GCF_000615285.1	s__Lactobacillus kitasatonis	80.9568	223	477	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus	95.0	98.87	97.76	0.93	0.87	3	-
GCF_000615445.1	s__Lactobacillus hamsteri	80.9291	261	477	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus	95.0	99.97	99.97	0.99	0.99	2	-
GCF_001436305.1	s__Lactobacillus ultunensis	80.4976	209	477	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus	95.0	99.98	99.95	0.99	0.98	4	-
GCF_004009905.1	s__Lactobacillus xujianguonis	80.4586	225	477	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus	95.0	99.83	99.82	0.94	0.92	3	-
GCF_900103655.1	s__Lactobacillus kefiranofaciens	80.4517	204	477	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus	95.0	99.62	99.18	0.95	0.85	9	-
GCF_001434335.1	s__Lactobacillus kalixensis	80.1668	229	477	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_018883635.1	s__Lactobacillus pullistercoris	80.1455	215	477	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001435325.1	s__Lactobacillus intestinalis	80.1397	195	477	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus	95.0	96.33	96.07	0.89	0.82	9	-
GCF_000297025.1	s__Lactobacillus pasteurii	79.6787	168	477	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus	95.0	99.99	99.98	0.99	0.99	3	-
GCA_910589675.1	s__Lactobacillus sp910589675	79.645	129	477	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lactobacillus	95.0	97.72	97.13	0.73	0.71	7	-
--------------------------------------------------------------------------------
[2024-01-24 14:20:19,242] [INFO] GTDB search result was written to GCF_000178475.1_ASM17847v1_genomic.fna/result_gtdb.tsv
[2024-01-24 14:20:19,243] [INFO] ===== GTDB Search completed =====
[2024-01-24 14:20:19,247] [INFO] DFAST_QC result json was written to GCF_000178475.1_ASM17847v1_genomic.fna/dqc_result.json
[2024-01-24 14:20:19,247] [INFO] DFAST_QC completed!
[2024-01-24 14:20:19,247] [INFO] Total running time: 0h0m35s