[2024-01-24 13:37:28,104] [INFO] DFAST_QC pipeline started.
[2024-01-24 13:37:28,108] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 13:37:28,108] [INFO] DQC Reference Directory: /var/lib/cwl/stg110ebdbe-63ae-444b-8e69-de1d48d11a27/dqc_reference
[2024-01-24 13:37:29,334] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 13:37:29,335] [INFO] Task started: Prodigal
[2024-01-24 13:37:29,335] [INFO] Running command: gunzip -c /var/lib/cwl/stg40ff0d22-f475-49cc-98de-d8ef6ab81932/GCF_000243455.1_ASM24345v2_genomic.fna.gz | prodigal -d GCF_000243455.1_ASM24345v2_genomic.fna/cds.fna -a GCF_000243455.1_ASM24345v2_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 13:37:32,649] [INFO] Task succeeded: Prodigal
[2024-01-24 13:37:32,650] [INFO] Task started: HMMsearch
[2024-01-24 13:37:32,650] [INFO] Running command: hmmsearch --tblout GCF_000243455.1_ASM24345v2_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg110ebdbe-63ae-444b-8e69-de1d48d11a27/dqc_reference/reference_markers.hmm GCF_000243455.1_ASM24345v2_genomic.fna/protein.faa > /dev/null
[2024-01-24 13:37:32,858] [INFO] Task succeeded: HMMsearch
[2024-01-24 13:37:32,859] [WARNING] Found 2/6 markers. [/var/lib/cwl/stg40ff0d22-f475-49cc-98de-d8ef6ab81932/GCF_000243455.1_ASM24345v2_genomic.fna.gz]
[2024-01-24 13:37:32,884] [INFO] Query marker FASTA was written to GCF_000243455.1_ASM24345v2_genomic.fna/markers.fasta
[2024-01-24 13:37:32,884] [INFO] Task started: Blastn
[2024-01-24 13:37:32,884] [INFO] Running command: blastn -query GCF_000243455.1_ASM24345v2_genomic.fna/markers.fasta -db /var/lib/cwl/stg110ebdbe-63ae-444b-8e69-de1d48d11a27/dqc_reference/reference_markers.fasta -out GCF_000243455.1_ASM24345v2_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:37:33,371] [INFO] Task succeeded: Blastn
[2024-01-24 13:37:33,375] [INFO] Selected 4 target genomes.
[2024-01-24 13:37:33,375] [INFO] Target genome list was writen to GCF_000243455.1_ASM24345v2_genomic.fna/target_genomes.txt
[2024-01-24 13:37:33,441] [INFO] Task started: fastANI
[2024-01-24 13:37:33,442] [INFO] Running command: fastANI --query /var/lib/cwl/stg40ff0d22-f475-49cc-98de-d8ef6ab81932/GCF_000243455.1_ASM24345v2_genomic.fna.gz --refList GCF_000243455.1_ASM24345v2_genomic.fna/target_genomes.txt --output GCF_000243455.1_ASM24345v2_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 13:37:35,458] [INFO] Task succeeded: fastANI
[2024-01-24 13:37:35,459] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg110ebdbe-63ae-444b-8e69-de1d48d11a27/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 13:37:35,459] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg110ebdbe-63ae-444b-8e69-de1d48d11a27/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 13:37:35,464] [INFO] Found 4 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 13:37:35,464] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 13:37:35,464] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Methanotorris formicicus	strain=Mc-S-70	GCA_000243455.2	213185	213185	type	True	100.0	544	549	95	conclusive
Methanotorris igneus	strain=Kol 5	GCA_000214415.1	2189	2189	type	True	85.9954	402	549	95	below_threshold
Methanocaldococcus vulcanius	strain=M7	GCA_000024625.1	73913	73913	type	True	77.9056	114	549	95	below_threshold
Methanocaldococcus fervens	strain=AG86	GCA_000023985.1	83171	83171	type	True	77.6619	136	549	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 13:37:35,466] [INFO] DFAST Taxonomy check result was written to GCF_000243455.1_ASM24345v2_genomic.fna/tc_result.tsv
[2024-01-24 13:37:35,467] [INFO] ===== Taxonomy check completed =====
[2024-01-24 13:37:35,467] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 13:37:35,467] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg110ebdbe-63ae-444b-8e69-de1d48d11a27/dqc_reference/checkm_data
[2024-01-24 13:37:35,468] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 13:37:35,493] [INFO] Task started: CheckM
[2024-01-24 13:37:35,493] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_000243455.1_ASM24345v2_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_000243455.1_ASM24345v2_genomic.fna/checkm_input GCF_000243455.1_ASM24345v2_genomic.fna/checkm_result
[2024-01-24 13:37:53,494] [INFO] Task succeeded: CheckM
[2024-01-24 13:37:53,496] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 95.83%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 13:37:53,521] [INFO] ===== Completeness check finished =====
[2024-01-24 13:37:53,522] [INFO] ===== Start GTDB Search =====
[2024-01-24 13:37:53,523] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_000243455.1_ASM24345v2_genomic.fna/markers.fasta)
[2024-01-24 13:37:53,523] [INFO] Task started: Blastn
[2024-01-24 13:37:53,523] [INFO] Running command: blastn -query GCF_000243455.1_ASM24345v2_genomic.fna/markers.fasta -db /var/lib/cwl/stg110ebdbe-63ae-444b-8e69-de1d48d11a27/dqc_reference/reference_markers_gtdb.fasta -out GCF_000243455.1_ASM24345v2_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:37:54,006] [INFO] Task succeeded: Blastn
[2024-01-24 13:37:54,017] [INFO] Selected 6 target genomes.
[2024-01-24 13:37:54,017] [INFO] Target genome list was writen to GCF_000243455.1_ASM24345v2_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 13:37:54,101] [INFO] Task started: fastANI
[2024-01-24 13:37:54,102] [INFO] Running command: fastANI --query /var/lib/cwl/stg40ff0d22-f475-49cc-98de-d8ef6ab81932/GCF_000243455.1_ASM24345v2_genomic.fna.gz --refList GCF_000243455.1_ASM24345v2_genomic.fna/target_genomes_gtdb.txt --output GCF_000243455.1_ASM24345v2_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 13:37:56,684] [INFO] Task succeeded: fastANI
[2024-01-24 13:37:56,692] [INFO] Found 5 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 13:37:56,693] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_000243455.1	s__Methanotorris formicicus	100.0	544	549	d__Archaea;p__Methanobacteriota_A;c__Methanococci;o__Methanococcales;f__Methanococcaceae;g__Methanotorris	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCF_000214415.1	s__Methanotorris igneus	86.0699	399	549	d__Archaea;p__Methanobacteriota_A;c__Methanococci;o__Methanococcales;f__Methanococcaceae;g__Methanotorris	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000023985.1	s__Methanocaldococcus fervens	77.5732	134	549	d__Archaea;p__Methanobacteriota_A;c__Methanococci;o__Methanococcales;f__Methanocaldococcaceae;g__Methanocaldococcus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_013153845.1	s__Methanocaldococcus sp013153845	77.1142	84	549	d__Archaea;p__Methanobacteriota_A;c__Methanococci;o__Methanococcales;f__Methanocaldococcaceae;g__Methanocaldococcus	95.0	N/A	N/A	N/A	N/A	1	-
GCA_015523155.1	s__Methanothermococcus_A sp015523155	76.9988	85	549	d__Archaea;p__Methanobacteriota_A;c__Methanococci;o__Methanococcales;f__Methanococcaceae;g__Methanothermococcus_A	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 13:37:56,694] [INFO] GTDB search result was written to GCF_000243455.1_ASM24345v2_genomic.fna/result_gtdb.tsv
[2024-01-24 13:37:56,695] [INFO] ===== GTDB Search completed =====
[2024-01-24 13:37:56,697] [INFO] DFAST_QC result json was written to GCF_000243455.1_ASM24345v2_genomic.fna/dqc_result.json
[2024-01-24 13:37:56,697] [INFO] DFAST_QC completed!
[2024-01-24 13:37:56,697] [INFO] Total running time: 0h0m29s