[2024-01-24 13:55:41,029] [INFO] DFAST_QC pipeline started.
[2024-01-24 13:55:41,031] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 13:55:41,031] [INFO] DQC Reference Directory: /var/lib/cwl/stgf42621d8-ec04-457b-9e45-108a52cabaa5/dqc_reference
[2024-01-24 13:55:42,398] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 13:55:42,399] [INFO] Task started: Prodigal
[2024-01-24 13:55:42,399] [INFO] Running command: gunzip -c /var/lib/cwl/stg8f40ccc4-60b6-49e0-bda3-1d928b09a92f/GCF_000266945.1_ASM26694v1_genomic.fna.gz | prodigal -d GCF_000266945.1_ASM26694v1_genomic.fna/cds.fna -a GCF_000266945.1_ASM26694v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 13:56:02,335] [INFO] Task succeeded: Prodigal
[2024-01-24 13:56:02,336] [INFO] Task started: HMMsearch
[2024-01-24 13:56:02,336] [INFO] Running command: hmmsearch --tblout GCF_000266945.1_ASM26694v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgf42621d8-ec04-457b-9e45-108a52cabaa5/dqc_reference/reference_markers.hmm GCF_000266945.1_ASM26694v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 13:56:02,673] [INFO] Task succeeded: HMMsearch
[2024-01-24 13:56:02,675] [INFO] Found 6/6 markers.
[2024-01-24 13:56:02,726] [INFO] Query marker FASTA was written to GCF_000266945.1_ASM26694v1_genomic.fna/markers.fasta
[2024-01-24 13:56:02,726] [INFO] Task started: Blastn
[2024-01-24 13:56:02,727] [INFO] Running command: blastn -query GCF_000266945.1_ASM26694v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgf42621d8-ec04-457b-9e45-108a52cabaa5/dqc_reference/reference_markers.fasta -out GCF_000266945.1_ASM26694v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:56:03,303] [INFO] Task succeeded: Blastn
[2024-01-24 13:56:03,307] [INFO] Selected 18 target genomes.
[2024-01-24 13:56:03,308] [INFO] Target genome list was writen to GCF_000266945.1_ASM26694v1_genomic.fna/target_genomes.txt
[2024-01-24 13:56:03,322] [INFO] Task started: fastANI
[2024-01-24 13:56:03,322] [INFO] Running command: fastANI --query /var/lib/cwl/stg8f40ccc4-60b6-49e0-bda3-1d928b09a92f/GCF_000266945.1_ASM26694v1_genomic.fna.gz --refList GCF_000266945.1_ASM26694v1_genomic.fna/target_genomes.txt --output GCF_000266945.1_ASM26694v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 13:56:20,878] [INFO] Task succeeded: fastANI
[2024-01-24 13:56:20,909] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgf42621d8-ec04-457b-9e45-108a52cabaa5/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 13:56:20,909] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgf42621d8-ec04-457b-9e45-108a52cabaa5/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 13:56:20,915] [INFO] Found 1 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 13:56:20,915] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 13:56:20,916] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Desulfomonile tiedjei	strain=DSM 6799	GCA_000266945.1	2358	2358	type	True	100.0	2174	2174	95	conclusive
--------------------------------------------------------------------------------
[2024-01-24 13:56:20,918] [INFO] DFAST Taxonomy check result was written to GCF_000266945.1_ASM26694v1_genomic.fna/tc_result.tsv
[2024-01-24 13:56:20,918] [INFO] ===== Taxonomy check completed =====
[2024-01-24 13:56:20,918] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 13:56:20,919] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgf42621d8-ec04-457b-9e45-108a52cabaa5/dqc_reference/checkm_data
[2024-01-24 13:56:20,921] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 13:56:20,982] [INFO] Task started: CheckM
[2024-01-24 13:56:20,982] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_000266945.1_ASM26694v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_000266945.1_ASM26694v1_genomic.fna/checkm_input GCF_000266945.1_ASM26694v1_genomic.fna/checkm_result
[2024-01-24 13:57:18,263] [INFO] Task succeeded: CheckM
[2024-01-24 13:57:18,265] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 13:57:18,288] [INFO] ===== Completeness check finished =====
[2024-01-24 13:57:18,289] [INFO] ===== Start GTDB Search =====
[2024-01-24 13:57:18,289] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_000266945.1_ASM26694v1_genomic.fna/markers.fasta)
[2024-01-24 13:57:18,289] [INFO] Task started: Blastn
[2024-01-24 13:57:18,290] [INFO] Running command: blastn -query GCF_000266945.1_ASM26694v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgf42621d8-ec04-457b-9e45-108a52cabaa5/dqc_reference/reference_markers_gtdb.fasta -out GCF_000266945.1_ASM26694v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:57:19,036] [INFO] Task succeeded: Blastn
[2024-01-24 13:57:19,040] [INFO] Selected 25 target genomes.
[2024-01-24 13:57:19,040] [INFO] Target genome list was writen to GCF_000266945.1_ASM26694v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 13:57:19,088] [INFO] Task started: fastANI
[2024-01-24 13:57:19,089] [INFO] Running command: fastANI --query /var/lib/cwl/stg8f40ccc4-60b6-49e0-bda3-1d928b09a92f/GCF_000266945.1_ASM26694v1_genomic.fna.gz --refList GCF_000266945.1_ASM26694v1_genomic.fna/target_genomes_gtdb.txt --output GCF_000266945.1_ASM26694v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 13:57:39,324] [INFO] Task succeeded: fastANI
[2024-01-24 13:57:39,328] [INFO] Found 2 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 13:57:39,328] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_000266945.1	s__Desulfomonile tiedjei	100.0	2172	2174	d__Bacteria;p__Desulfobacterota;c__Desulfomonilia;o__Desulfomonilales;f__Desulfomonilaceae;g__Desulfomonile	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCA_016212935.1	s__Desulfomonile tiedjei_A	77.8327	199	2174	d__Bacteria;p__Desulfobacterota;c__Desulfomonilia;o__Desulfomonilales;f__Desulfomonilaceae;g__Desulfomonile	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 13:57:39,329] [INFO] GTDB search result was written to GCF_000266945.1_ASM26694v1_genomic.fna/result_gtdb.tsv
[2024-01-24 13:57:39,330] [INFO] ===== GTDB Search completed =====
[2024-01-24 13:57:39,332] [INFO] DFAST_QC result json was written to GCF_000266945.1_ASM26694v1_genomic.fna/dqc_result.json
[2024-01-24 13:57:39,332] [INFO] DFAST_QC completed!
[2024-01-24 13:57:39,332] [INFO] Total running time: 0h1m58s