[2024-01-25 18:02:35,588] [INFO] DFAST_QC pipeline started.
[2024-01-25 18:02:35,589] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 18:02:35,589] [INFO] DQC Reference Directory: /var/lib/cwl/stg697bf435-5b60-4fa0-8221-b9f09956c68c/dqc_reference
[2024-01-25 18:02:36,833] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 18:02:36,833] [INFO] Task started: Prodigal
[2024-01-25 18:02:36,833] [INFO] Running command: gunzip -c /var/lib/cwl/stg8540aafb-0e27-40ec-b8e2-783f1214fd74/GCF_000292935.1_ASM29293v1_genomic.fna.gz | prodigal -d GCF_000292935.1_ASM29293v1_genomic.fna/cds.fna -a GCF_000292935.1_ASM29293v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 18:02:39,980] [INFO] Task succeeded: Prodigal
[2024-01-25 18:02:39,980] [INFO] Task started: HMMsearch
[2024-01-25 18:02:39,980] [INFO] Running command: hmmsearch --tblout GCF_000292935.1_ASM29293v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg697bf435-5b60-4fa0-8221-b9f09956c68c/dqc_reference/reference_markers.hmm GCF_000292935.1_ASM29293v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 18:02:40,198] [INFO] Task succeeded: HMMsearch
[2024-01-25 18:02:40,199] [INFO] Found 6/6 markers.
[2024-01-25 18:02:40,221] [INFO] Query marker FASTA was written to GCF_000292935.1_ASM29293v1_genomic.fna/markers.fasta
[2024-01-25 18:02:40,221] [INFO] Task started: Blastn
[2024-01-25 18:02:40,222] [INFO] Running command: blastn -query GCF_000292935.1_ASM29293v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg697bf435-5b60-4fa0-8221-b9f09956c68c/dqc_reference/reference_markers.fasta -out GCF_000292935.1_ASM29293v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 18:02:40,773] [INFO] Task succeeded: Blastn
[2024-01-25 18:02:40,775] [INFO] Selected 5 target genomes.
[2024-01-25 18:02:40,776] [INFO] Target genome list was writen to GCF_000292935.1_ASM29293v1_genomic.fna/target_genomes.txt
[2024-01-25 18:02:40,779] [INFO] Task started: fastANI
[2024-01-25 18:02:40,779] [INFO] Running command: fastANI --query /var/lib/cwl/stg8540aafb-0e27-40ec-b8e2-783f1214fd74/GCF_000292935.1_ASM29293v1_genomic.fna.gz --refList GCF_000292935.1_ASM29293v1_genomic.fna/target_genomes.txt --output GCF_000292935.1_ASM29293v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 18:02:44,754] [INFO] Task succeeded: fastANI
[2024-01-25 18:02:44,754] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg697bf435-5b60-4fa0-8221-b9f09956c68c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 18:02:44,754] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg697bf435-5b60-4fa0-8221-b9f09956c68c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 18:02:44,759] [INFO] Found 5 fastANI hits (2 hits with ANI > threshold)
[2024-01-25 18:02:44,759] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-25 18:02:44,759] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Fusobacterium hwasookii	strain=ChDC F128	GCA_000292935.1	1583098	1583098	type	True	100.0	776	777	95	conclusive
Fusobacterium hwasookii	strain=KCOM 1249	GCA_014217355.1	1583098	1583098	type	True	99.9185	774	777	95	conclusive
Fusobacterium nucleatum subsp. polymorphum	strain=ATCC 10953	GCA_000153625.1	76857	851	type	True	93.0354	649	777	95	below_threshold
Fusobacterium nucleatum subsp. polymorphum	strain=NCTC10562	GCA_001457555.1	76857	851	type	True	93.0157	652	777	95	below_threshold
Fusobacterium canifelinum	strain=FDAARGOS_1126	GCA_016724785.1	285729	285729	type	True	91.9307	634	777	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 18:02:44,761] [INFO] DFAST Taxonomy check result was written to GCF_000292935.1_ASM29293v1_genomic.fna/tc_result.tsv
[2024-01-25 18:02:44,761] [INFO] ===== Taxonomy check completed =====
[2024-01-25 18:02:44,761] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 18:02:44,762] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg697bf435-5b60-4fa0-8221-b9f09956c68c/dqc_reference/checkm_data
[2024-01-25 18:02:44,762] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 18:02:44,788] [INFO] Task started: CheckM
[2024-01-25 18:02:44,788] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_000292935.1_ASM29293v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_000292935.1_ASM29293v1_genomic.fna/checkm_input GCF_000292935.1_ASM29293v1_genomic.fna/checkm_result
[2024-01-25 18:03:00,394] [INFO] Task succeeded: CheckM
[2024-01-25 18:03:00,397] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 18:03:00,415] [INFO] ===== Completeness check finished =====
[2024-01-25 18:03:00,415] [INFO] ===== Start GTDB Search =====
[2024-01-25 18:03:00,415] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_000292935.1_ASM29293v1_genomic.fna/markers.fasta)
[2024-01-25 18:03:00,416] [INFO] Task started: Blastn
[2024-01-25 18:03:00,416] [INFO] Running command: blastn -query GCF_000292935.1_ASM29293v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg697bf435-5b60-4fa0-8221-b9f09956c68c/dqc_reference/reference_markers_gtdb.fasta -out GCF_000292935.1_ASM29293v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 18:03:01,147] [INFO] Task succeeded: Blastn
[2024-01-25 18:03:01,150] [INFO] Selected 9 target genomes.
[2024-01-25 18:03:01,150] [INFO] Target genome list was writen to GCF_000292935.1_ASM29293v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 18:03:01,156] [INFO] Task started: fastANI
[2024-01-25 18:03:01,157] [INFO] Running command: fastANI --query /var/lib/cwl/stg8540aafb-0e27-40ec-b8e2-783f1214fd74/GCF_000292935.1_ASM29293v1_genomic.fna.gz --refList GCF_000292935.1_ASM29293v1_genomic.fna/target_genomes_gtdb.txt --output GCF_000292935.1_ASM29293v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 18:03:07,377] [INFO] Task succeeded: fastANI
[2024-01-25 18:03:07,384] [INFO] Found 9 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 18:03:07,384] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_014217355.1	s__Fusobacterium hwasookii	99.9185	774	777	d__Bacteria;p__Fusobacteriota;c__Fusobacteriia;o__Fusobacteriales;f__Fusobacteriaceae;g__Fusobacterium	95.0	97.78	97.42	0.89	0.85	10	conclusive
GCF_001457555.1	s__Fusobacterium polymorphum	93.0157	652	777	d__Bacteria;p__Fusobacteriota;c__Fusobacteriia;o__Fusobacteriales;f__Fusobacteriaceae;g__Fusobacterium	95.0	96.55	95.37	0.85	0.79	30	-
GCF_002573625.1	s__Fusobacterium nucleatum_D	92.4881	631	777	d__Bacteria;p__Fusobacteriota;c__Fusobacteriia;o__Fusobacteriales;f__Fusobacteriaceae;g__Fusobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_016724785.1	s__Fusobacterium canifelinum	91.9688	632	777	d__Bacteria;p__Fusobacteriota;c__Fusobacteriia;o__Fusobacteriales;f__Fusobacteriaceae;g__Fusobacterium	95.0	98.22	98.13	0.90	0.87	3	-
GCA_000007325.1	s__Fusobacterium nucleatum	90.6858	573	777	d__Bacteria;p__Fusobacteriota;c__Fusobacteriia;o__Fusobacteriales;f__Fusobacteriaceae;g__Fusobacterium	95.0	98.30	95.23	0.93	0.85	13	-
GCA_000182945.1	s__Fusobacterium vincentii	89.4291	432	777	d__Bacteria;p__Fusobacteriota;c__Fusobacteriia;o__Fusobacteriales;f__Fusobacteriaceae;g__Fusobacterium	95.0	97.44	96.10	0.85	0.73	18	-
GCF_008633215.1	s__Fusobacterium nucleatum_J	89.1134	510	777	d__Bacteria;p__Fusobacteriota;c__Fusobacteriia;o__Fusobacteriales;f__Fusobacteriaceae;g__Fusobacterium	95.0	98.47	98.47	0.86	0.86	2	-
GCF_000158275.2	s__Fusobacterium animalis	88.8099	586	777	d__Bacteria;p__Fusobacteriota;c__Fusobacteriia;o__Fusobacteriales;f__Fusobacteriaceae;g__Fusobacterium	95.0	97.57	95.72	0.88	0.80	29	-
GCF_000493815.1	s__Fusobacterium nucleatum_E	87.7979	565	777	d__Bacteria;p__Fusobacteriota;c__Fusobacteriia;o__Fusobacteriales;f__Fusobacteriaceae;g__Fusobacterium	95.0	99.05	99.05	0.94	0.94	2	-
--------------------------------------------------------------------------------
[2024-01-25 18:03:07,385] [INFO] GTDB search result was written to GCF_000292935.1_ASM29293v1_genomic.fna/result_gtdb.tsv
[2024-01-25 18:03:07,386] [INFO] ===== GTDB Search completed =====
[2024-01-25 18:03:07,388] [INFO] DFAST_QC result json was written to GCF_000292935.1_ASM29293v1_genomic.fna/dqc_result.json
[2024-01-25 18:03:07,388] [INFO] DFAST_QC completed!
[2024-01-25 18:03:07,388] [INFO] Total running time: 0h0m32s