[2024-01-25 19:07:35,733] [INFO] DFAST_QC pipeline started.
[2024-01-25 19:07:35,734] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 19:07:35,734] [INFO] DQC Reference Directory: /var/lib/cwl/stg733ccea7-a3cd-4cec-b3c6-f4e59a52b1ce/dqc_reference
[2024-01-25 19:07:36,926] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 19:07:36,927] [INFO] Task started: Prodigal
[2024-01-25 19:07:36,927] [INFO] Running command: gunzip -c /var/lib/cwl/stg9482335d-054f-4e89-8761-d083bd0b2ab9/GCF_000337655.1_ASM33765v1_genomic.fna.gz | prodigal -d GCF_000337655.1_ASM33765v1_genomic.fna/cds.fna -a GCF_000337655.1_ASM33765v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 19:07:51,336] [INFO] Task succeeded: Prodigal
[2024-01-25 19:07:51,337] [INFO] Task started: HMMsearch
[2024-01-25 19:07:51,337] [INFO] Running command: hmmsearch --tblout GCF_000337655.1_ASM33765v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg733ccea7-a3cd-4cec-b3c6-f4e59a52b1ce/dqc_reference/reference_markers.hmm GCF_000337655.1_ASM33765v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 19:07:51,601] [INFO] Task succeeded: HMMsearch
[2024-01-25 19:07:51,602] [WARNING] Found 3/6 markers. [/var/lib/cwl/stg9482335d-054f-4e89-8761-d083bd0b2ab9/GCF_000337655.1_ASM33765v1_genomic.fna.gz]
[2024-01-25 19:07:51,632] [INFO] Query marker FASTA was written to GCF_000337655.1_ASM33765v1_genomic.fna/markers.fasta
[2024-01-25 19:07:51,632] [INFO] Task started: Blastn
[2024-01-25 19:07:51,632] [INFO] Running command: blastn -query GCF_000337655.1_ASM33765v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg733ccea7-a3cd-4cec-b3c6-f4e59a52b1ce/dqc_reference/reference_markers.fasta -out GCF_000337655.1_ASM33765v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 19:07:52,133] [INFO] Task succeeded: Blastn
[2024-01-25 19:07:52,136] [INFO] Selected 6 target genomes.
[2024-01-25 19:07:52,136] [INFO] Target genome list was writen to GCF_000337655.1_ASM33765v1_genomic.fna/target_genomes.txt
[2024-01-25 19:07:52,143] [INFO] Task started: fastANI
[2024-01-25 19:07:52,144] [INFO] Running command: fastANI --query /var/lib/cwl/stg9482335d-054f-4e89-8761-d083bd0b2ab9/GCF_000337655.1_ASM33765v1_genomic.fna.gz --refList GCF_000337655.1_ASM33765v1_genomic.fna/target_genomes.txt --output GCF_000337655.1_ASM33765v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 19:08:03,730] [INFO] Task succeeded: fastANI
[2024-01-25 19:08:03,730] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg733ccea7-a3cd-4cec-b3c6-f4e59a52b1ce/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 19:08:03,730] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg733ccea7-a3cd-4cec-b3c6-f4e59a52b1ce/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 19:08:03,736] [INFO] Found 6 fastANI hits (4 hits with ANI > threshold)
[2024-01-25 19:08:03,736] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-25 19:08:03,736] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Natronobacterium gregoryi	strain=SP2	GCA_000337655.1	44930	44930	type	True	100.0	1159	1169	95	conclusive
Natronobacterium gregoryi	strain=SP2	GCA_000230715.3	44930	44930	type	True	99.9743	1165	1169	95	conclusive
Natronobacterium gregoryi	strain=SP2	GCA_900114025.1	44930	44930	type	True	99.9723	1146	1169	95	conclusive
Natronobacterium gregoryi	strain=SP2	GCA_002855455.1	44930	44930	type	True	99.9254	1106	1169	95	conclusive
Natronobacterium texcoconense	strain=DSM 24767	GCA_900104065.1	1095778	1095778	type	True	85.1812	885	1169	95	below_threshold
Halobiforma haloterrestris	strain=DSM 13078	GCA_900112205.1	148448	148448	type	True	81.6588	783	1169	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 19:08:03,739] [INFO] DFAST Taxonomy check result was written to GCF_000337655.1_ASM33765v1_genomic.fna/tc_result.tsv
[2024-01-25 19:08:03,740] [INFO] ===== Taxonomy check completed =====
[2024-01-25 19:08:03,740] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 19:08:03,740] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg733ccea7-a3cd-4cec-b3c6-f4e59a52b1ce/dqc_reference/checkm_data
[2024-01-25 19:08:03,741] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 19:08:03,781] [INFO] Task started: CheckM
[2024-01-25 19:08:03,781] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_000337655.1_ASM33765v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_000337655.1_ASM33765v1_genomic.fna/checkm_input GCF_000337655.1_ASM33765v1_genomic.fna/checkm_result
[2024-01-25 19:08:45,141] [INFO] Task succeeded: CheckM
[2024-01-25 19:08:45,144] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 4.17%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 19:08:45,165] [INFO] ===== Completeness check finished =====
[2024-01-25 19:08:45,165] [INFO] ===== Start GTDB Search =====
[2024-01-25 19:08:45,166] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_000337655.1_ASM33765v1_genomic.fna/markers.fasta)
[2024-01-25 19:08:45,166] [INFO] Task started: Blastn
[2024-01-25 19:08:45,166] [INFO] Running command: blastn -query GCF_000337655.1_ASM33765v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg733ccea7-a3cd-4cec-b3c6-f4e59a52b1ce/dqc_reference/reference_markers_gtdb.fasta -out GCF_000337655.1_ASM33765v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 19:08:45,666] [INFO] Task succeeded: Blastn
[2024-01-25 19:08:45,670] [INFO] Selected 8 target genomes.
[2024-01-25 19:08:45,670] [INFO] Target genome list was writen to GCF_000337655.1_ASM33765v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 19:08:45,678] [INFO] Task started: fastANI
[2024-01-25 19:08:45,678] [INFO] Running command: fastANI --query /var/lib/cwl/stg9482335d-054f-4e89-8761-d083bd0b2ab9/GCF_000337655.1_ASM33765v1_genomic.fna.gz --refList GCF_000337655.1_ASM33765v1_genomic.fna/target_genomes_gtdb.txt --output GCF_000337655.1_ASM33765v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 19:08:54,726] [INFO] Task succeeded: fastANI
[2024-01-25 19:08:54,733] [INFO] Found 8 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 19:08:54,733] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_000230715.2	s__Natronobacterium gregoryi	99.9743	1165	1169	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Natrialbaceae;g__Natronobacterium	95.0	99.99	99.97	1.00	1.00	4	conclusive
GCF_900104065.1	s__Natronobacterium texcoconense	85.1718	886	1169	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Natrialbaceae;g__Natronobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000337895.1	s__Halobiforma nitratireducens	82.6283	674	1169	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Natrialbaceae;g__Halobiforma	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000226975.2	s__Halobiforma lacisalsi	81.7275	759	1169	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Natrialbaceae;g__Halobiforma	95.4693	99.98	99.98	1.00	1.00	2	-
GCF_900112205.1	s__Halobiforma haloterrestris	81.7151	776	1169	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Natrialbaceae;g__Halobiforma	95.4693	N/A	N/A	N/A	N/A	1	-
GCF_000217715.1	s__Halopiger xanaduensis	80.4077	618	1169	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Natrialbaceae;g__Halopiger	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003610195.1	s__Halopiger aswanensis	80.389	627	1169	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Natrialbaceae;g__Halopiger	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000455345.1	s__Halopiger_A goleimassiliensis	80.1973	621	1169	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Natrialbaceae;g__Halopiger_A	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-25 19:08:54,742] [INFO] GTDB search result was written to GCF_000337655.1_ASM33765v1_genomic.fna/result_gtdb.tsv
[2024-01-25 19:08:54,742] [INFO] ===== GTDB Search completed =====
[2024-01-25 19:08:54,745] [INFO] DFAST_QC result json was written to GCF_000337655.1_ASM33765v1_genomic.fna/dqc_result.json
[2024-01-25 19:08:54,745] [INFO] DFAST_QC completed!
[2024-01-25 19:08:54,745] [INFO] Total running time: 0h1m19s