[2024-01-25 18:04:35,748] [INFO] DFAST_QC pipeline started.
[2024-01-25 18:04:35,749] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 18:04:35,749] [INFO] DQC Reference Directory: /var/lib/cwl/stg2cd4fa63-b36b-4255-ae95-77c0f64c3015/dqc_reference
[2024-01-25 18:04:36,914] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 18:04:36,915] [INFO] Task started: Prodigal
[2024-01-25 18:04:36,915] [INFO] Running command: gunzip -c /var/lib/cwl/stg8c5ef1f5-681f-4fd9-8aee-d2bc0af76d58/GCF_000409755.1_Clostridium_butyricum_DSM_10702_Genome_sequencing_genomic.fna.gz | prodigal -d GCF_000409755.1_Clostridium_butyricum_DSM_10702_Genome_sequencing_genomic.fna/cds.fna -a GCF_000409755.1_Clostridium_butyricum_DSM_10702_Genome_sequencing_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 18:04:43,070] [INFO] Task succeeded: Prodigal
[2024-01-25 18:04:43,070] [INFO] Task started: HMMsearch
[2024-01-25 18:04:43,070] [INFO] Running command: hmmsearch --tblout GCF_000409755.1_Clostridium_butyricum_DSM_10702_Genome_sequencing_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg2cd4fa63-b36b-4255-ae95-77c0f64c3015/dqc_reference/reference_markers.hmm GCF_000409755.1_Clostridium_butyricum_DSM_10702_Genome_sequencing_genomic.fna/protein.faa > /dev/null
[2024-01-25 18:04:43,434] [INFO] Task succeeded: HMMsearch
[2024-01-25 18:04:43,435] [INFO] Found 6/6 markers.
[2024-01-25 18:04:43,486] [INFO] Query marker FASTA was written to GCF_000409755.1_Clostridium_butyricum_DSM_10702_Genome_sequencing_genomic.fna/markers.fasta
[2024-01-25 18:04:43,486] [INFO] Task started: Blastn
[2024-01-25 18:04:43,488] [INFO] Running command: blastn -query GCF_000409755.1_Clostridium_butyricum_DSM_10702_Genome_sequencing_genomic.fna/markers.fasta -db /var/lib/cwl/stg2cd4fa63-b36b-4255-ae95-77c0f64c3015/dqc_reference/reference_markers.fasta -out GCF_000409755.1_Clostridium_butyricum_DSM_10702_Genome_sequencing_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 18:04:44,080] [INFO] Task succeeded: Blastn
[2024-01-25 18:04:44,084] [INFO] Selected 24 target genomes.
[2024-01-25 18:04:44,085] [INFO] Target genome list was writen to GCF_000409755.1_Clostridium_butyricum_DSM_10702_Genome_sequencing_genomic.fna/target_genomes.txt
[2024-01-25 18:04:44,105] [INFO] Task started: fastANI
[2024-01-25 18:04:44,105] [INFO] Running command: fastANI --query /var/lib/cwl/stg8c5ef1f5-681f-4fd9-8aee-d2bc0af76d58/GCF_000409755.1_Clostridium_butyricum_DSM_10702_Genome_sequencing_genomic.fna.gz --refList GCF_000409755.1_Clostridium_butyricum_DSM_10702_Genome_sequencing_genomic.fna/target_genomes.txt --output GCF_000409755.1_Clostridium_butyricum_DSM_10702_Genome_sequencing_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 18:05:02,851] [INFO] Task succeeded: fastANI
[2024-01-25 18:05:02,852] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg2cd4fa63-b36b-4255-ae95-77c0f64c3015/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 18:05:02,852] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg2cd4fa63-b36b-4255-ae95-77c0f64c3015/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 18:05:02,864] [INFO] Found 19 fastANI hits (4 hits with ANI > threshold)
[2024-01-25 18:05:02,864] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-25 18:05:02,864] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Clostridium butyricum	strain=DSM 10702	GCA_000409755.1	1492	1492	type	True	100.0	1454	1459	95	conclusive
Clostridium butyricum	strain=DSM 10702	GCA_014131795.1	1492	1492	type	True	99.9986	1458	1459	95	conclusive
Clostridium butyricum	strain=NBRC 13949	GCA_006742065.1	1492	1492	type	True	99.9969	1458	1459	95	conclusive
Clostridium butyricum	strain=NBRC 13949	GCA_007992895.1	1492	1492	type	True	99.9839	1421	1459	95	conclusive
Clostridium saccharobutylicum	strain=DSM 13864	GCA_000473995.1	169679	169679	type	True	79.2598	513	1459	95	below_threshold
Clostridium saccharobutylicum	strain=DSM 13864	GCA_001657435.1	169679	169679	type	True	79.2517	467	1459	95	below_threshold
Clostridium neonatale	strain=LCDC 99A005	GCA_002553615.1	137838	137838	type	True	79.0256	527	1459	95	below_threshold
Clostridium beijerinckii	strain=DSM 791	GCA_018223745.1	1520	1520	suspected-type	True	78.7231	458	1459	95	below_threshold
Clostridium puniceum	strain=DSM 2619	GCA_002006345.1	29367	29367	type	True	78.7188	489	1459	95	below_threshold
Clostridium gelidum	strain=C5S11	GCA_019977655.1	704125	704125	type	True	78.6621	512	1459	95	below_threshold
Clostridium saccharoperbutylacetonicum	strain=N1-4(HMT)	GCA_000340885.1	36745	36745	type	True	78.5395	479	1459	95	below_threshold
Clostridium saccharoperbutylacetonicum	strain=ATCC 27021	GCA_000334435.1	36745	36745	type	True	78.5374	468	1459	95	below_threshold
Clostridium cagae	strain=Marseille-P4344	GCA_900290265.1	2080751	2080751	type	True	78.3269	395	1459	95	below_threshold
Clostridium cibarium	strain=Sa3CVN1	GCA_014836335.1	2762247	2762247	type	True	77.4319	227	1459	95	below_threshold
Clostridium chrysemydis	strain=PT	GCA_015234215.1	2665504	2665504	type	True	76.9708	197	1459	95	below_threshold
Clostridium muellerianum	strain=P21	GCA_012926525.1	2716538	2716538	type	True	75.9936	158	1459	95	below_threshold
Clostridium simiarum	strain=MSJ-4	GCA_018919175.1	2841506	2841506	type	True	75.8456	127	1459	95	below_threshold
Hathewaya histolytica	strain=NCTC503	GCA_901482605.1	1498	1498	type	True	75.845	82	1459	95	below_threshold
Malaciobacter halophilus	strain=DSM 18005	GCA_002837275.1	197482	197482	type	True	74.6466	52	1459	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 18:05:02,866] [INFO] DFAST Taxonomy check result was written to GCF_000409755.1_Clostridium_butyricum_DSM_10702_Genome_sequencing_genomic.fna/tc_result.tsv
[2024-01-25 18:05:02,866] [INFO] ===== Taxonomy check completed =====
[2024-01-25 18:05:02,866] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 18:05:02,867] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg2cd4fa63-b36b-4255-ae95-77c0f64c3015/dqc_reference/checkm_data
[2024-01-25 18:05:02,868] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 18:05:02,913] [INFO] Task started: CheckM
[2024-01-25 18:05:02,914] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_000409755.1_Clostridium_butyricum_DSM_10702_Genome_sequencing_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_000409755.1_Clostridium_butyricum_DSM_10702_Genome_sequencing_genomic.fna/checkm_input GCF_000409755.1_Clostridium_butyricum_DSM_10702_Genome_sequencing_genomic.fna/checkm_result
[2024-01-25 18:05:26,161] [INFO] Task succeeded: CheckM
[2024-01-25 18:05:26,162] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 18:05:26,176] [INFO] ===== Completeness check finished =====
[2024-01-25 18:05:26,176] [INFO] ===== Start GTDB Search =====
[2024-01-25 18:05:26,176] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_000409755.1_Clostridium_butyricum_DSM_10702_Genome_sequencing_genomic.fna/markers.fasta)
[2024-01-25 18:05:26,177] [INFO] Task started: Blastn
[2024-01-25 18:05:26,177] [INFO] Running command: blastn -query GCF_000409755.1_Clostridium_butyricum_DSM_10702_Genome_sequencing_genomic.fna/markers.fasta -db /var/lib/cwl/stg2cd4fa63-b36b-4255-ae95-77c0f64c3015/dqc_reference/reference_markers_gtdb.fasta -out GCF_000409755.1_Clostridium_butyricum_DSM_10702_Genome_sequencing_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 18:05:27,024] [INFO] Task succeeded: Blastn
[2024-01-25 18:05:27,028] [INFO] Selected 21 target genomes.
[2024-01-25 18:05:27,028] [INFO] Target genome list was writen to GCF_000409755.1_Clostridium_butyricum_DSM_10702_Genome_sequencing_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 18:05:27,043] [INFO] Task started: fastANI
[2024-01-25 18:05:27,044] [INFO] Running command: fastANI --query /var/lib/cwl/stg8c5ef1f5-681f-4fd9-8aee-d2bc0af76d58/GCF_000409755.1_Clostridium_butyricum_DSM_10702_Genome_sequencing_genomic.fna.gz --refList GCF_000409755.1_Clostridium_butyricum_DSM_10702_Genome_sequencing_genomic.fna/target_genomes_gtdb.txt --output GCF_000409755.1_Clostridium_butyricum_DSM_10702_Genome_sequencing_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 18:05:41,245] [INFO] Task succeeded: fastANI
[2024-01-25 18:05:41,254] [INFO] Found 14 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 18:05:41,255] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_006742065.1	s__Clostridium butyricum	99.9969	1458	1459	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium	95.0	98.58	97.46	0.89	0.80	64	conclusive
GCA_900769625.1	s__Clostridium sp900769625	83.2642	666	1459	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium	95.0	N/A	N/A	N/A	N/A	1	-
GCA_015058525.1	s__Clostridium butyricum_A	80.2735	369	1459	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_012843905.1	s__Clostridium sp012843905	80.0668	504	1459	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium	95.0	N/A	N/A	N/A	N/A	1	-
GCA_003539755.1	s__Clostridium sp003539755	79.5092	381	1459	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000473995.1	s__Clostridium saccharobutylicum	79.2877	510	1459	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium	95.0	99.89	95.82	0.99	0.83	65	-
GCF_001458595.1	s__Clostridium neonatale	79.0319	521	1459	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium	95.0	99.07	98.42	0.91	0.80	11	-
GCF_002006345.1	s__Clostridium puniceum	78.7199	491	1459	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_018223745.1	s__Clostridium beijerinckii	78.6957	460	1459	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium	95.0	97.01	95.18	0.85	0.79	244	-
GCF_000340885.1	s__Clostridium saccharoperbutylacetonicum	78.4942	483	1459	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium	95.0	99.35	98.00	0.96	0.89	7	-
GCF_900290265.1	s__Clostridium cagae	78.3373	394	1459	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium	95.0	98.09	97.56	0.90	0.85	15	-
GCF_001735765.2	s__Clostridium taeniosporum	78.2428	379	1459	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000827935.1	s__Clostridium botulinum_A	78.2218	402	1459	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Clostridium	95.0	99.05	97.92	0.91	0.85	31	-
GCF_901482605.1	s__Hathewaya histolytica	75.845	82	1459	d__Bacteria;p__Firmicutes_A;c__Clostridia;o__Clostridiales;f__Clostridiaceae;g__Hathewaya	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-25 18:05:41,256] [INFO] GTDB search result was written to GCF_000409755.1_Clostridium_butyricum_DSM_10702_Genome_sequencing_genomic.fna/result_gtdb.tsv
[2024-01-25 18:05:41,256] [INFO] ===== GTDB Search completed =====
[2024-01-25 18:05:41,261] [INFO] DFAST_QC result json was written to GCF_000409755.1_Clostridium_butyricum_DSM_10702_Genome_sequencing_genomic.fna/dqc_result.json
[2024-01-25 18:05:41,261] [INFO] DFAST_QC completed!
[2024-01-25 18:05:41,261] [INFO] Total running time: 0h1m6s