[2024-01-24 14:55:51,295] [INFO] DFAST_QC pipeline started.
[2024-01-24 14:55:51,296] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 14:55:51,297] [INFO] DQC Reference Directory: /var/lib/cwl/stg973f7067-34be-4e23-a3ff-5eb6ed98668a/dqc_reference
[2024-01-24 14:55:52,554] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 14:55:52,555] [INFO] Task started: Prodigal
[2024-01-24 14:55:52,555] [INFO] Running command: gunzip -c /var/lib/cwl/stg10c1e3ef-64c9-42ef-bb7a-749fb14683b5/GCF_000735355.1_GBAG_DRAFTv1_genomic.fna.gz | prodigal -d GCF_000735355.1_GBAG_DRAFTv1_genomic.fna/cds.fna -a GCF_000735355.1_GBAG_DRAFTv1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 14:56:05,699] [INFO] Task succeeded: Prodigal
[2024-01-24 14:56:05,700] [INFO] Task started: HMMsearch
[2024-01-24 14:56:05,700] [INFO] Running command: hmmsearch --tblout GCF_000735355.1_GBAG_DRAFTv1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg973f7067-34be-4e23-a3ff-5eb6ed98668a/dqc_reference/reference_markers.hmm GCF_000735355.1_GBAG_DRAFTv1_genomic.fna/protein.faa > /dev/null
[2024-01-24 14:56:05,989] [INFO] Task succeeded: HMMsearch
[2024-01-24 14:56:05,990] [INFO] Found 6/6 markers.
[2024-01-24 14:56:06,030] [INFO] Query marker FASTA was written to GCF_000735355.1_GBAG_DRAFTv1_genomic.fna/markers.fasta
[2024-01-24 14:56:06,031] [INFO] Task started: Blastn
[2024-01-24 14:56:06,031] [INFO] Running command: blastn -query GCF_000735355.1_GBAG_DRAFTv1_genomic.fna/markers.fasta -db /var/lib/cwl/stg973f7067-34be-4e23-a3ff-5eb6ed98668a/dqc_reference/reference_markers.fasta -out GCF_000735355.1_GBAG_DRAFTv1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:56:06,933] [INFO] Task succeeded: Blastn
[2024-01-24 14:56:06,936] [INFO] Selected 12 target genomes.
[2024-01-24 14:56:06,937] [INFO] Target genome list was writen to GCF_000735355.1_GBAG_DRAFTv1_genomic.fna/target_genomes.txt
[2024-01-24 14:56:06,942] [INFO] Task started: fastANI
[2024-01-24 14:56:06,942] [INFO] Running command: fastANI --query /var/lib/cwl/stg10c1e3ef-64c9-42ef-bb7a-749fb14683b5/GCF_000735355.1_GBAG_DRAFTv1_genomic.fna.gz --refList GCF_000735355.1_GBAG_DRAFTv1_genomic.fna/target_genomes.txt --output GCF_000735355.1_GBAG_DRAFTv1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 14:56:20,077] [INFO] Task succeeded: fastANI
[2024-01-24 14:56:20,078] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg973f7067-34be-4e23-a3ff-5eb6ed98668a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 14:56:20,078] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg973f7067-34be-4e23-a3ff-5eb6ed98668a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 14:56:20,093] [INFO] Found 12 fastANI hits (2 hits with ANI > threshold)
[2024-01-24 14:56:20,094] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 14:56:20,094] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Buttiauxella agrestis	strain=ATCC 33320	GCA_000735355.1	82977	82977	type	True	100.0	1523	1526	95	conclusive
Buttiauxella agrestis	strain=NCTC12119	GCA_900446255.1	82977	82977	type	True	96.3671	1402	1526	95	conclusive
Buttiauxella izardii	strain=CCUG 35510	GCA_003601925.1	82991	82991	type	True	89.2299	1278	1526	95	below_threshold
Buttiauxella brennerae	strain=ATCC 51605	GCA_001654925.1	82988	82988	type	True	87.4647	1224	1526	95	below_threshold
Buttiauxella massiliensis	strain=Marseille-P9829	GCA_902500225.1	2831590	2831590	type	True	87.412	1188	1526	95	below_threshold
Buttiauxella warmboldiae	strain=CCUG 35512	GCA_003818135.1	82993	82993	type	True	87.1429	981	1526	95	below_threshold
Buttiauxella ferragutiae	strain=ATCC 51602	GCA_001654915.1	82989	82989	type	True	86.6535	1165	1526	95	below_threshold
Buttiauxella noackiae	strain=ATCC 51607	GCA_001654865.1	82992	82992	type	True	86.5893	1151	1526	95	below_threshold
Pseudocitrobacter corydidari		GCA_021172065.1	2891570	2891570	type	True	79.7344	653	1526	95	below_threshold
Cronobacter sakazakii	strain=ATCC 29544	GCA_001971035.1	28141	28141	type	True	79.2427	577	1526	95	below_threshold
Citrobacter rodentium	strain=DSM 16636	GCA_021278985.1	67825	67825	type	True	79.1666	604	1526	95	below_threshold
Klebsiella pneumoniae	strain=PartO-Kpneumoniae-RM8376	GCA_022869665.1	573	573	type	True	79.1414	555	1526	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 14:56:20,095] [INFO] DFAST Taxonomy check result was written to GCF_000735355.1_GBAG_DRAFTv1_genomic.fna/tc_result.tsv
[2024-01-24 14:56:20,096] [INFO] ===== Taxonomy check completed =====
[2024-01-24 14:56:20,096] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 14:56:20,096] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg973f7067-34be-4e23-a3ff-5eb6ed98668a/dqc_reference/checkm_data
[2024-01-24 14:56:20,097] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 14:56:20,142] [INFO] Task started: CheckM
[2024-01-24 14:56:20,142] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_000735355.1_GBAG_DRAFTv1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_000735355.1_GBAG_DRAFTv1_genomic.fna/checkm_input GCF_000735355.1_GBAG_DRAFTv1_genomic.fna/checkm_result
[2024-01-24 14:57:03,007] [INFO] Task succeeded: CheckM
[2024-01-24 14:57:03,009] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 14:57:03,028] [INFO] ===== Completeness check finished =====
[2024-01-24 14:57:03,029] [INFO] ===== Start GTDB Search =====
[2024-01-24 14:57:03,029] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_000735355.1_GBAG_DRAFTv1_genomic.fna/markers.fasta)
[2024-01-24 14:57:03,030] [INFO] Task started: Blastn
[2024-01-24 14:57:03,030] [INFO] Running command: blastn -query GCF_000735355.1_GBAG_DRAFTv1_genomic.fna/markers.fasta -db /var/lib/cwl/stg973f7067-34be-4e23-a3ff-5eb6ed98668a/dqc_reference/reference_markers_gtdb.fasta -out GCF_000735355.1_GBAG_DRAFTv1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:57:04,413] [INFO] Task succeeded: Blastn
[2024-01-24 14:57:04,417] [INFO] Selected 9 target genomes.
[2024-01-24 14:57:04,417] [INFO] Target genome list was writen to GCF_000735355.1_GBAG_DRAFTv1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 14:57:04,425] [INFO] Task started: fastANI
[2024-01-24 14:57:04,425] [INFO] Running command: fastANI --query /var/lib/cwl/stg10c1e3ef-64c9-42ef-bb7a-749fb14683b5/GCF_000735355.1_GBAG_DRAFTv1_genomic.fna.gz --refList GCF_000735355.1_GBAG_DRAFTv1_genomic.fna/target_genomes_gtdb.txt --output GCF_000735355.1_GBAG_DRAFTv1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 14:57:16,441] [INFO] Task succeeded: fastANI
[2024-01-24 14:57:16,453] [INFO] Found 9 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 14:57:16,454] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_000735355.1	s__Buttiauxella agrestis	100.0	1523	1526	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Buttiauxella	95.0	96.92	96.37	0.91	0.90	3	conclusive
GCF_003601925.1	s__Buttiauxella izardii	89.2299	1278	1526	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Buttiauxella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001654925.1	s__Buttiauxella brennerae	87.4647	1224	1526	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Buttiauxella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_902500225.1	s__Buttiauxella agrestis_A	87.4011	1189	1526	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Buttiauxella	95.0	95.69	95.69	0.85	0.85	2	-
GCF_003818135.1	s__Buttiauxella warmboldiae	87.1359	982	1526	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Buttiauxella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001654835.1	s__Buttiauxella gaviniae	87.1056	1207	1526	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Buttiauxella	95.0	98.46	98.46	0.87	0.87	2	-
GCF_001654915.1	s__Buttiauxella ferragutiae	86.6535	1165	1526	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Buttiauxella	95.0	99.06	98.93	0.93	0.91	3	-
GCF_001654865.1	s__Buttiauxella noackiae	86.5901	1151	1526	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Buttiauxella	95.0	98.49	98.49	0.92	0.92	2	-
GCF_006376615.1	s__Buttiauxella sp006376615	85.6891	1144	1526	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Buttiauxella	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 14:57:16,455] [INFO] GTDB search result was written to GCF_000735355.1_GBAG_DRAFTv1_genomic.fna/result_gtdb.tsv
[2024-01-24 14:57:16,458] [INFO] ===== GTDB Search completed =====
[2024-01-24 14:57:16,463] [INFO] DFAST_QC result json was written to GCF_000735355.1_GBAG_DRAFTv1_genomic.fna/dqc_result.json
[2024-01-24 14:57:16,464] [INFO] DFAST_QC completed!
[2024-01-24 14:57:16,464] [INFO] Total running time: 0h1m25s