[2024-01-24 13:32:56,165] [INFO] DFAST_QC pipeline started.
[2024-01-24 13:32:56,167] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 13:32:56,167] [INFO] DQC Reference Directory: /var/lib/cwl/stg1e616dc7-4d36-49d8-8b2d-dd5c81585632/dqc_reference
[2024-01-24 13:32:57,532] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 13:32:57,533] [INFO] Task started: Prodigal
[2024-01-24 13:32:57,533] [INFO] Running command: gunzip -c /var/lib/cwl/stgf7515ca5-1e59-438a-b871-3170faf487ea/GCF_000737145.1_ASM73714v1_genomic.fna.gz | prodigal -d GCF_000737145.1_ASM73714v1_genomic.fna/cds.fna -a GCF_000737145.1_ASM73714v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 13:33:06,454] [INFO] Task succeeded: Prodigal
[2024-01-24 13:33:06,455] [INFO] Task started: HMMsearch
[2024-01-24 13:33:06,455] [INFO] Running command: hmmsearch --tblout GCF_000737145.1_ASM73714v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg1e616dc7-4d36-49d8-8b2d-dd5c81585632/dqc_reference/reference_markers.hmm GCF_000737145.1_ASM73714v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 13:33:06,726] [INFO] Task succeeded: HMMsearch
[2024-01-24 13:33:06,728] [INFO] Found 6/6 markers.
[2024-01-24 13:33:06,764] [INFO] Query marker FASTA was written to GCF_000737145.1_ASM73714v1_genomic.fna/markers.fasta
[2024-01-24 13:33:06,765] [INFO] Task started: Blastn
[2024-01-24 13:33:06,765] [INFO] Running command: blastn -query GCF_000737145.1_ASM73714v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg1e616dc7-4d36-49d8-8b2d-dd5c81585632/dqc_reference/reference_markers.fasta -out GCF_000737145.1_ASM73714v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:33:07,413] [INFO] Task succeeded: Blastn
[2024-01-24 13:33:07,417] [INFO] Selected 8 target genomes.
[2024-01-24 13:33:07,417] [INFO] Target genome list was writen to GCF_000737145.1_ASM73714v1_genomic.fna/target_genomes.txt
[2024-01-24 13:33:07,423] [INFO] Task started: fastANI
[2024-01-24 13:33:07,423] [INFO] Running command: fastANI --query /var/lib/cwl/stgf7515ca5-1e59-438a-b871-3170faf487ea/GCF_000737145.1_ASM73714v1_genomic.fna.gz --refList GCF_000737145.1_ASM73714v1_genomic.fna/target_genomes.txt --output GCF_000737145.1_ASM73714v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 13:33:15,282] [INFO] Task succeeded: fastANI
[2024-01-24 13:33:15,283] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg1e616dc7-4d36-49d8-8b2d-dd5c81585632/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 13:33:15,283] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg1e616dc7-4d36-49d8-8b2d-dd5c81585632/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 13:33:15,292] [INFO] Found 8 fastANI hits (5 hits with ANI > threshold)
[2024-01-24 13:33:15,292] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 13:33:15,292] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Acinetobacter baumannii	strain=ATCC 19606	GCA_000737145.1	470	470	type	True	100.0	1317	1317	95	conclusive
Acinetobacter baumannii	strain=ATCC 19606	GCA_014116795.1	470	470	type	True	99.9804	1293	1317	95	conclusive
Acinetobacter baumannii	strain=ATCC 19606	GCA_020911985.1	470	470	type	True	99.9775	1312	1317	95	conclusive
Acinetobacter baumannii	strain=PartI-Abaumannii-RM8376	GCA_022870045.1	470	470	type	True	99.9758	1310	1317	95	conclusive
Acinetobacter baumannii	strain=ATCC 19606	GCA_009035845.1	470	470	type	True	99.9726	1315	1317	95	conclusive
Acinetobacter pittii	strain=CIP70.29	GCA_024390955.1	48296	48296	type	True	89.2134	1040	1317	95	below_threshold
Acinetobacter junii	strain=NCTC10307	GCA_900444875.1	40215	40215	type	True	80.2775	479	1317	95	below_threshold
Acinetobacter pecorum	strain=Sa1BUA6	GCA_014837015.1	2762215	2762215	type	True	78.8232	295	1317	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 13:33:15,294] [INFO] DFAST Taxonomy check result was written to GCF_000737145.1_ASM73714v1_genomic.fna/tc_result.tsv
[2024-01-24 13:33:15,295] [INFO] ===== Taxonomy check completed =====
[2024-01-24 13:33:15,295] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 13:33:15,295] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg1e616dc7-4d36-49d8-8b2d-dd5c81585632/dqc_reference/checkm_data
[2024-01-24 13:33:15,297] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 13:33:15,342] [INFO] Task started: CheckM
[2024-01-24 13:33:15,343] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_000737145.1_ASM73714v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_000737145.1_ASM73714v1_genomic.fna/checkm_input GCF_000737145.1_ASM73714v1_genomic.fna/checkm_result
[2024-01-24 13:33:48,681] [INFO] Task succeeded: CheckM
[2024-01-24 13:33:48,682] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 13:33:48,704] [INFO] ===== Completeness check finished =====
[2024-01-24 13:33:48,704] [INFO] ===== Start GTDB Search =====
[2024-01-24 13:33:48,704] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_000737145.1_ASM73714v1_genomic.fna/markers.fasta)
[2024-01-24 13:33:48,705] [INFO] Task started: Blastn
[2024-01-24 13:33:48,705] [INFO] Running command: blastn -query GCF_000737145.1_ASM73714v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg1e616dc7-4d36-49d8-8b2d-dd5c81585632/dqc_reference/reference_markers_gtdb.fasta -out GCF_000737145.1_ASM73714v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:33:49,586] [INFO] Task succeeded: Blastn
[2024-01-24 13:33:49,589] [INFO] Selected 11 target genomes.
[2024-01-24 13:33:49,590] [INFO] Target genome list was writen to GCF_000737145.1_ASM73714v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 13:33:49,604] [INFO] Task started: fastANI
[2024-01-24 13:33:49,604] [INFO] Running command: fastANI --query /var/lib/cwl/stgf7515ca5-1e59-438a-b871-3170faf487ea/GCF_000737145.1_ASM73714v1_genomic.fna.gz --refList GCF_000737145.1_ASM73714v1_genomic.fna/target_genomes_gtdb.txt --output GCF_000737145.1_ASM73714v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 13:33:59,949] [INFO] Task succeeded: fastANI
[2024-01-24 13:33:59,965] [INFO] Found 11 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 13:33:59,965] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_009759685.1	s__Acinetobacter baumannii	99.9722	1313	1317	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	97.86	96.67	0.89	0.80	5417	conclusive
GCF_000368085.1	s__Acinetobacter nosocomialis	92.0084	1090	1317	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	97.94	97.50	0.90	0.85	250	-
GCF_000368065.1	s__Acinetobacter seifertii	90.2094	1067	1317	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	96.88	96.48	0.87	0.81	81	-
GCF_002928115.1	s__Acinetobacter pittii_H	89.2239	1079	1317	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	96.46	96.15	0.91	0.87	9	-
GCF_001605885.1	s__Acinetobacter lactucae	88.6461	1038	1317	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	97.34	96.55	0.91	0.85	16	-
GCF_013344765.1	s__Acinetobacter lactucae_A	88.5133	871	1317	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	97.51	97.14	0.90	0.87	4	-
GCF_000399685.1	s__Acinetobacter pittii_E	88.4081	1063	1317	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000196795.1	s__Acinetobacter oleivorans	88.0863	1092	1317	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	96.22	95.12	0.90	0.85	40	-
GCF_000313935.1	s__Acinetobacter sp000313935	88.0717	1062	1317	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	99.37	99.37	0.94	0.94	2	-
GCF_000399665.1	s__Acinetobacter calcoaceticus_B	87.6612	1048	1317	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_016508255.1	s__Acinetobacter calcoaceticus_E	87.317	1018	1317	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Acinetobacter	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 13:33:59,967] [INFO] GTDB search result was written to GCF_000737145.1_ASM73714v1_genomic.fna/result_gtdb.tsv
[2024-01-24 13:33:59,967] [INFO] ===== GTDB Search completed =====
[2024-01-24 13:33:59,970] [INFO] DFAST_QC result json was written to GCF_000737145.1_ASM73714v1_genomic.fna/dqc_result.json
[2024-01-24 13:33:59,971] [INFO] DFAST_QC completed!
[2024-01-24 13:33:59,971] [INFO] Total running time: 0h1m4s