[2024-01-24 12:25:20,609] [INFO] DFAST_QC pipeline started.
[2024-01-24 12:25:20,611] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 12:25:20,611] [INFO] DQC Reference Directory: /var/lib/cwl/stgfc304beb-e524-45bd-aa94-8939559f394a/dqc_reference
[2024-01-24 12:25:21,838] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 12:25:21,839] [INFO] Task started: Prodigal
[2024-01-24 12:25:21,839] [INFO] Running command: gunzip -c /var/lib/cwl/stg5bfd9dff-e26e-4413-ba9c-55f94ea84d1c/GCF_000746025.2_ASM74602v2_genomic.fna.gz | prodigal -d GCF_000746025.2_ASM74602v2_genomic.fna/cds.fna -a GCF_000746025.2_ASM74602v2_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 12:25:30,774] [INFO] Task succeeded: Prodigal
[2024-01-24 12:25:30,775] [INFO] Task started: HMMsearch
[2024-01-24 12:25:30,775] [INFO] Running command: hmmsearch --tblout GCF_000746025.2_ASM74602v2_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgfc304beb-e524-45bd-aa94-8939559f394a/dqc_reference/reference_markers.hmm GCF_000746025.2_ASM74602v2_genomic.fna/protein.faa > /dev/null
[2024-01-24 12:25:31,045] [INFO] Task succeeded: HMMsearch
[2024-01-24 12:25:31,046] [INFO] Found 6/6 markers.
[2024-01-24 12:25:31,086] [INFO] Query marker FASTA was written to GCF_000746025.2_ASM74602v2_genomic.fna/markers.fasta
[2024-01-24 12:25:31,086] [INFO] Task started: Blastn
[2024-01-24 12:25:31,086] [INFO] Running command: blastn -query GCF_000746025.2_ASM74602v2_genomic.fna/markers.fasta -db /var/lib/cwl/stgfc304beb-e524-45bd-aa94-8939559f394a/dqc_reference/reference_markers.fasta -out GCF_000746025.2_ASM74602v2_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:25:31,646] [INFO] Task succeeded: Blastn
[2024-01-24 12:25:31,649] [INFO] Selected 16 target genomes.
[2024-01-24 12:25:31,650] [INFO] Target genome list was writen to GCF_000746025.2_ASM74602v2_genomic.fna/target_genomes.txt
[2024-01-24 12:25:31,656] [INFO] Task started: fastANI
[2024-01-24 12:25:31,656] [INFO] Running command: fastANI --query /var/lib/cwl/stg5bfd9dff-e26e-4413-ba9c-55f94ea84d1c/GCF_000746025.2_ASM74602v2_genomic.fna.gz --refList GCF_000746025.2_ASM74602v2_genomic.fna/target_genomes.txt --output GCF_000746025.2_ASM74602v2_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 12:25:40,268] [INFO] Task succeeded: fastANI
[2024-01-24 12:25:40,269] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgfc304beb-e524-45bd-aa94-8939559f394a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 12:25:40,269] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgfc304beb-e524-45bd-aa94-8939559f394a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 12:25:40,275] [INFO] Found 1 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 12:25:40,275] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 12:25:40,275] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Thermanaerosceptrum fracticalcis	strain=DRI-13	GCA_000746025.2	1712410	1712410	type	True	100.0	1264	1268	95	conclusive
--------------------------------------------------------------------------------
[2024-01-24 12:25:40,278] [INFO] DFAST Taxonomy check result was written to GCF_000746025.2_ASM74602v2_genomic.fna/tc_result.tsv
[2024-01-24 12:25:40,279] [INFO] ===== Taxonomy check completed =====
[2024-01-24 12:25:40,279] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 12:25:40,280] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgfc304beb-e524-45bd-aa94-8939559f394a/dqc_reference/checkm_data
[2024-01-24 12:25:40,280] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 12:25:40,317] [INFO] Task started: CheckM
[2024-01-24 12:25:40,317] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_000746025.2_ASM74602v2_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_000746025.2_ASM74602v2_genomic.fna/checkm_input GCF_000746025.2_ASM74602v2_genomic.fna/checkm_result
[2024-01-24 12:26:11,266] [INFO] Task succeeded: CheckM
[2024-01-24 12:26:11,267] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 4.17%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 12:26:11,292] [INFO] ===== Completeness check finished =====
[2024-01-24 12:26:11,293] [INFO] ===== Start GTDB Search =====
[2024-01-24 12:26:11,293] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_000746025.2_ASM74602v2_genomic.fna/markers.fasta)
[2024-01-24 12:26:11,293] [INFO] Task started: Blastn
[2024-01-24 12:26:11,294] [INFO] Running command: blastn -query GCF_000746025.2_ASM74602v2_genomic.fna/markers.fasta -db /var/lib/cwl/stgfc304beb-e524-45bd-aa94-8939559f394a/dqc_reference/reference_markers_gtdb.fasta -out GCF_000746025.2_ASM74602v2_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:26:12,005] [INFO] Task succeeded: Blastn
[2024-01-24 12:26:12,008] [INFO] Selected 20 target genomes.
[2024-01-24 12:26:12,008] [INFO] Target genome list was writen to GCF_000746025.2_ASM74602v2_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 12:26:12,025] [INFO] Task started: fastANI
[2024-01-24 12:26:12,026] [INFO] Running command: fastANI --query /var/lib/cwl/stg5bfd9dff-e26e-4413-ba9c-55f94ea84d1c/GCF_000746025.2_ASM74602v2_genomic.fna.gz --refList GCF_000746025.2_ASM74602v2_genomic.fna/target_genomes_gtdb.txt --output GCF_000746025.2_ASM74602v2_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 12:26:20,959] [INFO] Task succeeded: fastANI
[2024-01-24 12:26:20,964] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 12:26:20,964] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_000746025.2	s__Thermanaerosceptrum fracticalcis	100.0	1263	1268	d__Bacteria;p__Firmicutes_B;c__Peptococcia;o__DRI-13;f__DRI-13;g__Thermanaerosceptrum	95.0	N/A	N/A	N/A	N/A	1	conclusive
--------------------------------------------------------------------------------
[2024-01-24 12:26:20,966] [INFO] GTDB search result was written to GCF_000746025.2_ASM74602v2_genomic.fna/result_gtdb.tsv
[2024-01-24 12:26:20,967] [INFO] ===== GTDB Search completed =====
[2024-01-24 12:26:20,970] [INFO] DFAST_QC result json was written to GCF_000746025.2_ASM74602v2_genomic.fna/dqc_result.json
[2024-01-24 12:26:20,970] [INFO] DFAST_QC completed!
[2024-01-24 12:26:20,970] [INFO] Total running time: 0h1m0s