[2024-01-25 18:47:35,573] [INFO] DFAST_QC pipeline started.
[2024-01-25 18:47:35,574] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 18:47:35,574] [INFO] DQC Reference Directory: /var/lib/cwl/stg145c3dd9-4acb-4684-af49-c28ccb11dde8/dqc_reference
[2024-01-25 18:47:36,729] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 18:47:36,730] [INFO] Task started: Prodigal
[2024-01-25 18:47:36,730] [INFO] Running command: gunzip -c /var/lib/cwl/stgcabefe8b-982f-4aa1-8229-31307c9c0811/GCF_000759735.1_ASM75973v1_genomic.fna.gz | prodigal -d GCF_000759735.1_ASM75973v1_genomic.fna/cds.fna -a GCF_000759735.1_ASM75973v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 18:47:48,371] [INFO] Task succeeded: Prodigal
[2024-01-25 18:47:48,371] [INFO] Task started: HMMsearch
[2024-01-25 18:47:48,372] [INFO] Running command: hmmsearch --tblout GCF_000759735.1_ASM75973v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg145c3dd9-4acb-4684-af49-c28ccb11dde8/dqc_reference/reference_markers.hmm GCF_000759735.1_ASM75973v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 18:47:48,693] [INFO] Task succeeded: HMMsearch
[2024-01-25 18:47:48,694] [INFO] Found 6/6 markers.
[2024-01-25 18:47:48,743] [INFO] Query marker FASTA was written to GCF_000759735.1_ASM75973v1_genomic.fna/markers.fasta
[2024-01-25 18:47:48,745] [INFO] Task started: Blastn
[2024-01-25 18:47:48,745] [INFO] Running command: blastn -query GCF_000759735.1_ASM75973v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg145c3dd9-4acb-4684-af49-c28ccb11dde8/dqc_reference/reference_markers.fasta -out GCF_000759735.1_ASM75973v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 18:47:49,618] [INFO] Task succeeded: Blastn
[2024-01-25 18:47:49,621] [INFO] Selected 11 target genomes.
[2024-01-25 18:47:49,621] [INFO] Target genome list was writen to GCF_000759735.1_ASM75973v1_genomic.fna/target_genomes.txt
[2024-01-25 18:47:49,623] [INFO] Task started: fastANI
[2024-01-25 18:47:49,623] [INFO] Running command: fastANI --query /var/lib/cwl/stgcabefe8b-982f-4aa1-8229-31307c9c0811/GCF_000759735.1_ASM75973v1_genomic.fna.gz --refList GCF_000759735.1_ASM75973v1_genomic.fna/target_genomes.txt --output GCF_000759735.1_ASM75973v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 18:48:02,215] [INFO] Task succeeded: fastANI
[2024-01-25 18:48:02,216] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg145c3dd9-4acb-4684-af49-c28ccb11dde8/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 18:48:02,216] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg145c3dd9-4acb-4684-af49-c28ccb11dde8/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 18:48:02,223] [INFO] Found 11 fastANI hits (4 hits with ANI > threshold)
[2024-01-25 18:48:02,224] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-25 18:48:02,224] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Citrobacter freundii	strain=NBRC 12681	GCA_000759735.1	546	546	type	True	100.0	1611	1612	95	conclusive
Citrobacter freundii	strain=ATCC 8090	GCA_011064845.1	546	546	type	True	99.9996	1611	1612	95	conclusive
Citrobacter freundii	strain=MTCC 1658	GCA_000312465.1	546	546	type	True	99.9913	1599	1612	95	conclusive
Citrobacter freundii	strain=ATCC 8090	GCA_000734905.1	546	546	type	True	99.9632	1551	1612	95	conclusive
Citrobacter portucalensis	strain=A60	GCA_002042885.1	1639133	1639133	type	True	94.756	1377	1612	95	below_threshold
Citrobacter arsenatis	strain=LY-1	GCA_004353845.1	2546350	2546350	type	True	92.5803	1414	1612	95	below_threshold
Citrobacter rodentium	strain=DSM 16636	GCA_021278985.1	67825	67825	type	True	82.8813	1013	1612	95	below_threshold
Salmonella enterica subsp. enterica	strain=PartC-Senterica-RM8376	GCA_022869965.1	59201	28901	suspected-type	True	82.8613	941	1612	95	below_threshold
Enterobacter hormaechei	strain=FDAARGOS 1433	GCA_019048245.1	158836	158836	suspected-type	True	81.6283	820	1612	95	below_threshold
Enterobacter wuhouensis	strain=WCHEW120002	GCA_004331265.1	2529381	2529381	type	True	81.5697	868	1612	95	below_threshold
Enterobacter roggenkampii	strain=DSM 16690	GCA_024390995.1	1812935	1812935	type	True	81.5695	851	1612	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 18:48:02,226] [INFO] DFAST Taxonomy check result was written to GCF_000759735.1_ASM75973v1_genomic.fna/tc_result.tsv
[2024-01-25 18:48:02,226] [INFO] ===== Taxonomy check completed =====
[2024-01-25 18:48:02,226] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 18:48:02,227] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg145c3dd9-4acb-4684-af49-c28ccb11dde8/dqc_reference/checkm_data
[2024-01-25 18:48:02,227] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 18:48:02,277] [INFO] Task started: CheckM
[2024-01-25 18:48:02,277] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_000759735.1_ASM75973v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_000759735.1_ASM75973v1_genomic.fna/checkm_input GCF_000759735.1_ASM75973v1_genomic.fna/checkm_result
[2024-01-25 18:48:38,805] [INFO] Task succeeded: CheckM
[2024-01-25 18:48:38,806] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 18:48:38,829] [INFO] ===== Completeness check finished =====
[2024-01-25 18:48:38,829] [INFO] ===== Start GTDB Search =====
[2024-01-25 18:48:38,830] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_000759735.1_ASM75973v1_genomic.fna/markers.fasta)
[2024-01-25 18:48:38,830] [INFO] Task started: Blastn
[2024-01-25 18:48:38,830] [INFO] Running command: blastn -query GCF_000759735.1_ASM75973v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg145c3dd9-4acb-4684-af49-c28ccb11dde8/dqc_reference/reference_markers_gtdb.fasta -out GCF_000759735.1_ASM75973v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 18:48:40,231] [INFO] Task succeeded: Blastn
[2024-01-25 18:48:40,235] [INFO] Selected 8 target genomes.
[2024-01-25 18:48:40,235] [INFO] Target genome list was writen to GCF_000759735.1_ASM75973v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 18:48:40,237] [INFO] Task started: fastANI
[2024-01-25 18:48:40,237] [INFO] Running command: fastANI --query /var/lib/cwl/stgcabefe8b-982f-4aa1-8229-31307c9c0811/GCF_000759735.1_ASM75973v1_genomic.fna.gz --refList GCF_000759735.1_ASM75973v1_genomic.fna/target_genomes_gtdb.txt --output GCF_000759735.1_ASM75973v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 18:48:49,535] [INFO] Task succeeded: fastANI
[2024-01-25 18:48:49,541] [INFO] Found 8 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 18:48:49,542] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_011064845.1	s__Citrobacter freundii	99.9996	1611	1612	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Citrobacter	95.0	98.70	97.40	0.89	0.80	446	conclusive
GCF_002042885.1	s__Citrobacter portucalensis	94.756	1377	1612	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Citrobacter	95.0	98.01	95.95	0.89	0.83	128	-
GCF_900079995.3	s__Citrobacter europaeus	92.8032	1410	1612	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Citrobacter	95.0	99.13	99.04	0.92	0.90	21	-
GCF_018035235.1	s__Citrobacter freundii_A	92.7159	1345	1612	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Citrobacter	95.0	99.33	99.24	0.91	0.90	6	-
GCF_004353845.1	s__Citrobacter freundii_E	92.5849	1413	1612	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Citrobacter	95.0	99.27	99.27	0.92	0.92	2	-
GCF_002075345.1	s__Citrobacter braakii	92.3794	1350	1612	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Citrobacter	95.0	98.63	96.30	0.86	0.77	154	-
GCF_003818115.1	s__Citrobacter youngae	91.7435	1330	1612	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Citrobacter	95.0	97.80	95.31	0.91	0.87	45	-
GCA_900759445.1	s__Klebsiella sp900759445	86.7009	723	1612	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Klebsiella	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-25 18:48:49,543] [INFO] GTDB search result was written to GCF_000759735.1_ASM75973v1_genomic.fna/result_gtdb.tsv
[2024-01-25 18:48:49,543] [INFO] ===== GTDB Search completed =====
[2024-01-25 18:48:49,546] [INFO] DFAST_QC result json was written to GCF_000759735.1_ASM75973v1_genomic.fna/dqc_result.json
[2024-01-25 18:48:49,546] [INFO] DFAST_QC completed!
[2024-01-25 18:48:49,546] [INFO] Total running time: 0h1m14s