[2024-01-24 12:53:41,198] [INFO] DFAST_QC pipeline started.
[2024-01-24 12:53:41,200] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 12:53:41,201] [INFO] DQC Reference Directory: /var/lib/cwl/stg1f5f454f-4360-4279-83af-268600923045/dqc_reference
[2024-01-24 12:53:42,703] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 12:53:42,704] [INFO] Task started: Prodigal
[2024-01-24 12:53:42,705] [INFO] Running command: gunzip -c /var/lib/cwl/stg7f536acf-17fa-413d-81ba-fad0d27d0cf9/GCF_000813705.1_ASM81370v1_genomic.fna.gz | prodigal -d GCF_000813705.1_ASM81370v1_genomic.fna/cds.fna -a GCF_000813705.1_ASM81370v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 12:53:49,565] [INFO] Task succeeded: Prodigal
[2024-01-24 12:53:49,565] [INFO] Task started: HMMsearch
[2024-01-24 12:53:49,565] [INFO] Running command: hmmsearch --tblout GCF_000813705.1_ASM81370v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg1f5f454f-4360-4279-83af-268600923045/dqc_reference/reference_markers.hmm GCF_000813705.1_ASM81370v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 12:53:49,927] [INFO] Task succeeded: HMMsearch
[2024-01-24 12:53:49,929] [INFO] Found 6/6 markers.
[2024-01-24 12:53:49,958] [INFO] Query marker FASTA was written to GCF_000813705.1_ASM81370v1_genomic.fna/markers.fasta
[2024-01-24 12:53:49,959] [INFO] Task started: Blastn
[2024-01-24 12:53:49,959] [INFO] Running command: blastn -query GCF_000813705.1_ASM81370v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg1f5f454f-4360-4279-83af-268600923045/dqc_reference/reference_markers.fasta -out GCF_000813705.1_ASM81370v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:53:51,415] [INFO] Task succeeded: Blastn
[2024-01-24 12:53:51,418] [INFO] Selected 12 target genomes.
[2024-01-24 12:53:51,419] [INFO] Target genome list was writen to GCF_000813705.1_ASM81370v1_genomic.fna/target_genomes.txt
[2024-01-24 12:53:51,426] [INFO] Task started: fastANI
[2024-01-24 12:53:51,426] [INFO] Running command: fastANI --query /var/lib/cwl/stg7f536acf-17fa-413d-81ba-fad0d27d0cf9/GCF_000813705.1_ASM81370v1_genomic.fna.gz --refList GCF_000813705.1_ASM81370v1_genomic.fna/target_genomes.txt --output GCF_000813705.1_ASM81370v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 12:54:11,678] [INFO] Task succeeded: fastANI
[2024-01-24 12:54:11,679] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg1f5f454f-4360-4279-83af-268600923045/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 12:54:11,679] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg1f5f454f-4360-4279-83af-268600923045/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 12:54:11,695] [WARNING] Following organisms are indistinguishable with ANI. [Morococcus cerebrosus(1056807), Neisseria mucosa(488), Neisseria sicca(490)]
[2024-01-24 12:54:11,695] [INFO] Found 12 fastANI hits (2 hits with ANI > threshold)
[2024-01-24 12:54:11,696] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 12:54:11,696] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Morococcus cerebrosus	strain=CIP 81.93	GCA_000813705.1	1056807	1056807	type	True	100.0	726	733	95	conclusive
Morococcus cerebrosus	strain=CIP 81.93	GCA_022749515.1	1056807	1056807	type	True	99.9771	730	733	95	conclusive
Neisseria sicca	strain=DSM 17713	GCA_014054945.1	490	490	suspected-type	True	93.9357	593	733	95	below_threshold
Neisseria sicca	strain=ATCC 29256	GCA_000174655.1	490	490	suspected-type	True	93.9003	595	733	95	below_threshold
Neisseria cinerea	strain=CCUG 2156	GCA_021025975.1	483	483	type	True	86.5668	379	733	95	below_threshold
Neisseria meningitidis	strain=PartJ-Nmeningitidis-RM8376	GCA_022869645.1	487	487	type	True	85.7898	446	733	95	below_threshold
Neisseria subflava	strain=ATCC 49275	GCA_005221305.1	28449	28449	type	True	85.007	456	733	95	below_threshold
Neisseria flavescens	strain=NCTC8263	GCA_900453805.1	484	484	type	True	84.619	454	733	95	below_threshold
Neisseria elongata subsp. elongata	strain=ATCC 25295	GCA_900113545.1	214482	495	type	True	84.5004	347	733	95	below_threshold
Neisseria elongata subsp. glycolytica	strain=NCTC11050	GCA_900475815.1	88719	495	type	True	84.3718	346	733	95	below_threshold
Neisseria elongata	strain=NCTC10660	GCA_900453895.1	495	495	type	True	84.3394	349	733	95	below_threshold
Neisseria perflava	strain=CCUG 17915	GCA_023472875.1	33053	33053	suspected-type	True	83.9489	435	733	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 12:54:11,698] [INFO] DFAST Taxonomy check result was written to GCF_000813705.1_ASM81370v1_genomic.fna/tc_result.tsv
[2024-01-24 12:54:11,698] [INFO] ===== Taxonomy check completed =====
[2024-01-24 12:54:11,698] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 12:54:11,699] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg1f5f454f-4360-4279-83af-268600923045/dqc_reference/checkm_data
[2024-01-24 12:54:11,700] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 12:54:11,728] [INFO] Task started: CheckM
[2024-01-24 12:54:11,728] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_000813705.1_ASM81370v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_000813705.1_ASM81370v1_genomic.fna/checkm_input GCF_000813705.1_ASM81370v1_genomic.fna/checkm_result
[2024-01-24 12:54:37,178] [INFO] Task succeeded: CheckM
[2024-01-24 12:54:37,180] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 99.62%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 12:54:37,202] [INFO] ===== Completeness check finished =====
[2024-01-24 12:54:37,202] [INFO] ===== Start GTDB Search =====
[2024-01-24 12:54:37,202] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_000813705.1_ASM81370v1_genomic.fna/markers.fasta)
[2024-01-24 12:54:37,203] [INFO] Task started: Blastn
[2024-01-24 12:54:37,203] [INFO] Running command: blastn -query GCF_000813705.1_ASM81370v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg1f5f454f-4360-4279-83af-268600923045/dqc_reference/reference_markers_gtdb.fasta -out GCF_000813705.1_ASM81370v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:54:38,569] [INFO] Task succeeded: Blastn
[2024-01-24 12:54:38,573] [INFO] Selected 12 target genomes.
[2024-01-24 12:54:38,573] [INFO] Target genome list was writen to GCF_000813705.1_ASM81370v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 12:54:38,599] [INFO] Task started: fastANI
[2024-01-24 12:54:38,599] [INFO] Running command: fastANI --query /var/lib/cwl/stg7f536acf-17fa-413d-81ba-fad0d27d0cf9/GCF_000813705.1_ASM81370v1_genomic.fna.gz --refList GCF_000813705.1_ASM81370v1_genomic.fna/target_genomes_gtdb.txt --output GCF_000813705.1_ASM81370v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 12:55:01,396] [INFO] Task succeeded: fastANI
[2024-01-24 12:55:01,454] [INFO] Found 12 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 12:55:01,454] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_000813705.1	s__Neisseria cerebrosa	100.0	726	733	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Neisseria	96.5429	97.92	97.52	0.91	0.89	5	conclusive
GCA_003028315.1	s__Neisseria mucosa	96.5429	671	733	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Neisseria	96.5429	96.97	96.70	0.92	0.90	9	conclusive
GCF_000952795.1	s__Neisseria sp000952795	96.149	627	733	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Neisseria	96.4916	98.25	96.69	0.90	0.83	3	-
GCF_003044425.1	s__Neisseria sicca_C	95.7412	626	733	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Neisseria	95.8064	96.78	96.77	0.93	0.92	3	-
GCF_017753665.1	s__Neisseria sicca_D	94.9761	664	733	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Neisseria	95.2415	96.44	95.35	0.92	0.88	6	-
GCF_003044445.1	s__Neisseria mucosa_A	94.6239	640	733	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Neisseria	95.2415	96.25	95.81	0.90	0.87	15	-
GCF_000193735.1	s__Neisseria sicca_E	94.1907	598	733	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Neisseria	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000260655.1	s__Neisseria sicca_B	94.028	536	733	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Neisseria	96.5569	97.07	96.76	0.92	0.90	3	-
GCF_014054945.1	s__Neisseria sicca	93.9794	591	733	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Neisseria	96.5569	99.97	99.97	1.00	1.00	2	-
GCF_000227275.1	s__Neisseria sp000227275	93.9399	586	733	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Neisseria	96.3387	97.52	96.71	0.95	0.93	3	-
GCA_000220865.1	s__Neisseria macacae	93.8821	588	733	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Neisseria	96.509	99.99	99.99	0.98	0.98	2	-
GCF_005886145.1	s__Neisseria sp000090875	87.5466	464	733	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Burkholderiales;f__Neisseriaceae;g__Neisseria	95.0	98.69	97.41	0.97	0.94	3	-
--------------------------------------------------------------------------------
[2024-01-24 12:55:01,456] [INFO] GTDB search result was written to GCF_000813705.1_ASM81370v1_genomic.fna/result_gtdb.tsv
[2024-01-24 12:55:01,456] [INFO] ===== GTDB Search completed =====
[2024-01-24 12:55:01,460] [INFO] DFAST_QC result json was written to GCF_000813705.1_ASM81370v1_genomic.fna/dqc_result.json
[2024-01-24 12:55:01,460] [INFO] DFAST_QC completed!
[2024-01-24 12:55:01,460] [INFO] Total running time: 0h1m20s