[2024-01-24 11:51:37,325] [INFO] DFAST_QC pipeline started.
[2024-01-24 11:51:37,328] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 11:51:37,328] [INFO] DQC Reference Directory: /var/lib/cwl/stga8e02a89-28c4-4bdb-9151-50fb54cdaf2f/dqc_reference
[2024-01-24 11:51:38,608] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 11:51:38,609] [INFO] Task started: Prodigal
[2024-01-24 11:51:38,609] [INFO] Running command: gunzip -c /var/lib/cwl/stga14a6900-3f8b-4116-9fc6-4306fb2a6d50/GCF_000836725.1_ASM83672v1_genomic.fna.gz | prodigal -d GCF_000836725.1_ASM83672v1_genomic.fna/cds.fna -a GCF_000836725.1_ASM83672v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 11:51:45,102] [INFO] Task succeeded: Prodigal
[2024-01-24 11:51:45,102] [INFO] Task started: HMMsearch
[2024-01-24 11:51:45,103] [INFO] Running command: hmmsearch --tblout GCF_000836725.1_ASM83672v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stga8e02a89-28c4-4bdb-9151-50fb54cdaf2f/dqc_reference/reference_markers.hmm GCF_000836725.1_ASM83672v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 11:51:45,351] [INFO] Task succeeded: HMMsearch
[2024-01-24 11:51:45,352] [INFO] Found 6/6 markers.
[2024-01-24 11:51:45,382] [INFO] Query marker FASTA was written to GCF_000836725.1_ASM83672v1_genomic.fna/markers.fasta
[2024-01-24 11:51:45,382] [INFO] Task started: Blastn
[2024-01-24 11:51:45,383] [INFO] Running command: blastn -query GCF_000836725.1_ASM83672v1_genomic.fna/markers.fasta -db /var/lib/cwl/stga8e02a89-28c4-4bdb-9151-50fb54cdaf2f/dqc_reference/reference_markers.fasta -out GCF_000836725.1_ASM83672v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:51:46,025] [INFO] Task succeeded: Blastn
[2024-01-24 11:51:46,029] [INFO] Selected 13 target genomes.
[2024-01-24 11:51:46,030] [INFO] Target genome list was writen to GCF_000836725.1_ASM83672v1_genomic.fna/target_genomes.txt
[2024-01-24 11:51:46,036] [INFO] Task started: fastANI
[2024-01-24 11:51:46,036] [INFO] Running command: fastANI --query /var/lib/cwl/stga14a6900-3f8b-4116-9fc6-4306fb2a6d50/GCF_000836725.1_ASM83672v1_genomic.fna.gz --refList GCF_000836725.1_ASM83672v1_genomic.fna/target_genomes.txt --output GCF_000836725.1_ASM83672v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 11:51:54,917] [INFO] Task succeeded: fastANI
[2024-01-24 11:51:54,917] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stga8e02a89-28c4-4bdb-9151-50fb54cdaf2f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 11:51:54,918] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stga8e02a89-28c4-4bdb-9151-50fb54cdaf2f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 11:51:54,927] [INFO] Found 10 fastANI hits (2 hits with ANI > threshold)
[2024-01-24 11:51:54,927] [INFO] The taxonomy check result is classified as 'inconclusive'.
[2024-01-24 11:51:54,927] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Anoxybacillus thermarum	strain=AF/04	GCA_000836725.1	404937	404937	type	True	100.0	836	842	95	inconclusive
Anoxybacillus kamchatkensis	strain=DSM 14988	GCA_013761015.1	196180	196180	type	True	95.0786	692	842	95	inconclusive
Anoxybacillus ayderensis	strain=AB04	GCA_000833605.1	265546	265546	type	True	94.732	725	842	95	below_threshold
Anoxybacillus gonensis	strain=G2	GCA_000770375.1	198467	198467	suspected-type	True	93.7025	729	842	95	below_threshold
Anoxybacillus gonensis	strain=G2	GCA_001187595.1	198467	198467	suspected-type	True	93.611	735	842	95	below_threshold
Anoxybacillus karvacharensis	strain=K1	GCA_001996285.1	1919277	1919277	type	True	87.7048	691	842	95	below_threshold
Anoxybacillus mongoliensis	strain=DSM 19169	GCA_014201515.1	452565	452565	type	True	87.5403	707	842	95	below_threshold
Anoxybacillus kestanbolensis	strain=NCIMB 13971	GCA_023653015.1	227476	227476	type	True	87.5245	684	842	95	below_threshold
Anoxybacillus tengchongensis	strain=DSM 23211	GCA_014201585.1	576944	576944	type	True	86.8326	705	842	95	below_threshold
Neobacillus kokaensis	strain=LOB 377	GCA_014656545.1	2759023	2759023	type	True	76.5341	57	842	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 11:51:54,929] [INFO] DFAST Taxonomy check result was written to GCF_000836725.1_ASM83672v1_genomic.fna/tc_result.tsv
[2024-01-24 11:51:54,929] [INFO] ===== Taxonomy check completed =====
[2024-01-24 11:51:54,929] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 11:51:54,930] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stga8e02a89-28c4-4bdb-9151-50fb54cdaf2f/dqc_reference/checkm_data
[2024-01-24 11:51:54,931] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 11:51:54,958] [INFO] Task started: CheckM
[2024-01-24 11:51:54,958] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_000836725.1_ASM83672v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_000836725.1_ASM83672v1_genomic.fna/checkm_input GCF_000836725.1_ASM83672v1_genomic.fna/checkm_result
[2024-01-24 11:52:20,991] [INFO] Task succeeded: CheckM
[2024-01-24 11:52:20,993] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 11:52:21,015] [INFO] ===== Completeness check finished =====
[2024-01-24 11:52:21,015] [INFO] ===== Start GTDB Search =====
[2024-01-24 11:52:21,016] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_000836725.1_ASM83672v1_genomic.fna/markers.fasta)
[2024-01-24 11:52:21,016] [INFO] Task started: Blastn
[2024-01-24 11:52:21,016] [INFO] Running command: blastn -query GCF_000836725.1_ASM83672v1_genomic.fna/markers.fasta -db /var/lib/cwl/stga8e02a89-28c4-4bdb-9151-50fb54cdaf2f/dqc_reference/reference_markers_gtdb.fasta -out GCF_000836725.1_ASM83672v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:52:22,086] [INFO] Task succeeded: Blastn
[2024-01-24 11:52:22,090] [INFO] Selected 9 target genomes.
[2024-01-24 11:52:22,090] [INFO] Target genome list was writen to GCF_000836725.1_ASM83672v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 11:52:22,098] [INFO] Task started: fastANI
[2024-01-24 11:52:22,098] [INFO] Running command: fastANI --query /var/lib/cwl/stga14a6900-3f8b-4116-9fc6-4306fb2a6d50/GCF_000836725.1_ASM83672v1_genomic.fna.gz --refList GCF_000836725.1_ASM83672v1_genomic.fna/target_genomes_gtdb.txt --output GCF_000836725.1_ASM83672v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 11:52:28,753] [INFO] Task succeeded: fastANI
[2024-01-24 11:52:28,762] [INFO] Found 9 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 11:52:28,762] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_000836725.1	s__Anoxybacillus thermarum	100.0	836	842	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Anoxybacillaceae;g__Anoxybacillus	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCF_000833605.1	s__Anoxybacillus ayderensis	94.6717	728	842	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Anoxybacillaceae;g__Anoxybacillus	95.0	97.55	97.17	0.91	0.88	8	-
GCF_001187595.1	s__Anoxybacillus gonensis	93.5879	736	842	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Anoxybacillaceae;g__Anoxybacillus	95.0	98.51	96.49	0.91	0.88	6	-
GCF_002742685.1	s__Anoxybacillus flavithermus_E	89.671	685	842	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Anoxybacillaceae;g__Anoxybacillus	95.0	99.92	99.92	0.93	0.93	2	-
GCA_002243705.1	s__Anoxybacillus flavithermus	87.6326	709	842	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Anoxybacillaceae;g__Anoxybacillus	95.0	96.37	95.30	0.87	0.86	4	-
GCF_014201515.1	s__Anoxybacillus mongoliensis	87.5448	706	842	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Anoxybacillaceae;g__Anoxybacillus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002197485.1	s__Anoxybacillus flavithermus_G	86.9293	662	842	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Anoxybacillaceae;g__Anoxybacillus	95.0	98.60	95.08	0.91	0.76	28	-
GCF_014201585.1	s__Anoxybacillus tengchongensis	86.845	704	842	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Anoxybacillaceae;g__Anoxybacillus	95.0	96.46	96.29	0.90	0.87	4	-
GCF_900111795.1	s__Anoxybacillus pushchinoensis	86.5894	609	842	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales;f__Anoxybacillaceae;g__Anoxybacillus	95.0	95.71	95.71	0.84	0.84	2	-
--------------------------------------------------------------------------------
[2024-01-24 11:52:28,764] [INFO] GTDB search result was written to GCF_000836725.1_ASM83672v1_genomic.fna/result_gtdb.tsv
[2024-01-24 11:52:28,764] [INFO] ===== GTDB Search completed =====
[2024-01-24 11:52:28,767] [INFO] DFAST_QC result json was written to GCF_000836725.1_ASM83672v1_genomic.fna/dqc_result.json
[2024-01-24 11:52:28,767] [INFO] DFAST_QC completed!
[2024-01-24 11:52:28,768] [INFO] Total running time: 0h0m51s