[2024-01-24 12:46:49,489] [INFO] DFAST_QC pipeline started.
[2024-01-24 12:46:49,491] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 12:46:49,491] [INFO] DQC Reference Directory: /var/lib/cwl/stg4dd2d992-6e50-4972-9615-dbd9e2d8440b/dqc_reference
[2024-01-24 12:46:50,621] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 12:46:50,621] [INFO] Task started: Prodigal
[2024-01-24 12:46:50,622] [INFO] Running command: gunzip -c /var/lib/cwl/stgd9c8dfa1-1c2a-49c9-a342-d5f3ea7a95f5/GCF_001025175.1_ASM102517v1_genomic.fna.gz | prodigal -d GCF_001025175.1_ASM102517v1_genomic.fna/cds.fna -a GCF_001025175.1_ASM102517v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 12:46:58,410] [INFO] Task succeeded: Prodigal
[2024-01-24 12:46:58,410] [INFO] Task started: HMMsearch
[2024-01-24 12:46:58,410] [INFO] Running command: hmmsearch --tblout GCF_001025175.1_ASM102517v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg4dd2d992-6e50-4972-9615-dbd9e2d8440b/dqc_reference/reference_markers.hmm GCF_001025175.1_ASM102517v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 12:46:58,588] [INFO] Task succeeded: HMMsearch
[2024-01-24 12:46:58,589] [INFO] Found 6/6 markers.
[2024-01-24 12:46:58,609] [INFO] Query marker FASTA was written to GCF_001025175.1_ASM102517v1_genomic.fna/markers.fasta
[2024-01-24 12:46:58,609] [INFO] Task started: Blastn
[2024-01-24 12:46:58,610] [INFO] Running command: blastn -query GCF_001025175.1_ASM102517v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg4dd2d992-6e50-4972-9615-dbd9e2d8440b/dqc_reference/reference_markers.fasta -out GCF_001025175.1_ASM102517v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:46:59,431] [INFO] Task succeeded: Blastn
[2024-01-24 12:46:59,434] [INFO] Selected 13 target genomes.
[2024-01-24 12:46:59,434] [INFO] Target genome list was writen to GCF_001025175.1_ASM102517v1_genomic.fna/target_genomes.txt
[2024-01-24 12:46:59,450] [INFO] Task started: fastANI
[2024-01-24 12:46:59,450] [INFO] Running command: fastANI --query /var/lib/cwl/stgd9c8dfa1-1c2a-49c9-a342-d5f3ea7a95f5/GCF_001025175.1_ASM102517v1_genomic.fna.gz --refList GCF_001025175.1_ASM102517v1_genomic.fna/target_genomes.txt --output GCF_001025175.1_ASM102517v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 12:47:06,336] [INFO] Task succeeded: fastANI
[2024-01-24 12:47:06,336] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg4dd2d992-6e50-4972-9615-dbd9e2d8440b/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 12:47:06,337] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg4dd2d992-6e50-4972-9615-dbd9e2d8440b/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 12:47:06,347] [INFO] Found 13 fastANI hits (4 hits with ANI > threshold)
[2024-01-24 12:47:06,347] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 12:47:06,348] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Bifidobacterium breve	strain=JCM 1192	GCA_001025175.1	1685	1685	type	True	100.0	755	756	95	conclusive
Bifidobacterium breve	strain=NCTC11815	GCA_900637145.1	1685	1685	type	True	99.9953	755	756	95	conclusive
Bifidobacterium breve	strain=DSM 20213	GCA_000158015.1	1685	1685	type	True	99.9872	752	756	95	conclusive
Bifidobacterium breve	strain=DSM 20213	GCA_000771305.1	1685	1685	type	True	99.9743	735	756	95	conclusive
Bifidobacterium longum subsp. infantis	strain=DSM 20088	GCA_000771105.1	1682	216816	type	True	87.595	524	756	95	below_threshold
Bifidobacterium longum subsp. suillum	strain=SU-851	GCA_016882605.1	1931217	216816	type	True	87.5874	515	756	95	below_threshold
Bifidobacterium longum subsp. longum	strain=JCM 1217	GCA_000196555.1	1679	216816	type	True	86.871	522	756	95	below_threshold
Bifidobacterium miconisargentati	strain=82T25	GCA_019331675.1	2834437	2834437	type	True	83.1829	482	756	95	below_threshold
Bifidobacterium myosotis	strain=DSM 100196	GCA_002259745.1	1630166	1630166	type	True	82.9146	457	756	95	below_threshold
Bifidobacterium amazonense	strain=MA1	GCA_018555435.2	2809027	2809027	type	True	81.2371	357	756	95	below_threshold
Bifidobacterium pseudolongum subsp. pseudolongum	strain=ATCC 25526	GCA_022691205.1	31954	1694	type	True	78.7699	211	756	95	below_threshold
Bifidobacterium mizhiense	strain=S053-2	GCA_020884755.1	2879940	2879940	type	True	77.7614	133	756	95	below_threshold
Georgenia yuyongxinii	strain=Z443	GCA_006352065.1	2589797	2589797	type	True	76.9072	54	756	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 12:47:06,349] [INFO] DFAST Taxonomy check result was written to GCF_001025175.1_ASM102517v1_genomic.fna/tc_result.tsv
[2024-01-24 12:47:06,350] [INFO] ===== Taxonomy check completed =====
[2024-01-24 12:47:06,350] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 12:47:06,350] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg4dd2d992-6e50-4972-9615-dbd9e2d8440b/dqc_reference/checkm_data
[2024-01-24 12:47:06,351] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 12:47:06,376] [INFO] Task started: CheckM
[2024-01-24 12:47:06,377] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_001025175.1_ASM102517v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_001025175.1_ASM102517v1_genomic.fna/checkm_input GCF_001025175.1_ASM102517v1_genomic.fna/checkm_result
[2024-01-24 12:47:34,446] [INFO] Task succeeded: CheckM
[2024-01-24 12:47:34,447] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 12:47:34,464] [INFO] ===== Completeness check finished =====
[2024-01-24 12:47:34,465] [INFO] ===== Start GTDB Search =====
[2024-01-24 12:47:34,465] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_001025175.1_ASM102517v1_genomic.fna/markers.fasta)
[2024-01-24 12:47:34,465] [INFO] Task started: Blastn
[2024-01-24 12:47:34,465] [INFO] Running command: blastn -query GCF_001025175.1_ASM102517v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg4dd2d992-6e50-4972-9615-dbd9e2d8440b/dqc_reference/reference_markers_gtdb.fasta -out GCF_001025175.1_ASM102517v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:47:35,719] [INFO] Task succeeded: Blastn
[2024-01-24 12:47:35,722] [INFO] Selected 12 target genomes.
[2024-01-24 12:47:35,722] [INFO] Target genome list was writen to GCF_001025175.1_ASM102517v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 12:47:35,730] [INFO] Task started: fastANI
[2024-01-24 12:47:35,730] [INFO] Running command: fastANI --query /var/lib/cwl/stgd9c8dfa1-1c2a-49c9-a342-d5f3ea7a95f5/GCF_001025175.1_ASM102517v1_genomic.fna.gz --refList GCF_001025175.1_ASM102517v1_genomic.fna/target_genomes_gtdb.txt --output GCF_001025175.1_ASM102517v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 12:47:42,017] [INFO] Task succeeded: fastANI
[2024-01-24 12:47:42,027] [INFO] Found 11 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 12:47:42,027] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_001025175.1	s__Bifidobacterium breve	100.0	755	756	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	98.58	97.75	0.90	0.86	170	conclusive
GCF_000269965.1	s__Bifidobacterium infantis	87.6291	553	756	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	98.68	97.44	0.91	0.82	49	-
GCF_000196555.1	s__Bifidobacterium longum	86.8651	525	756	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	98.53	95.59	0.86	0.67	481	-
GCF_018555355.1	s__Bifidobacterium sp018555355	83.1798	485	756	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	96.26	96.26	0.85	0.85	2	-
GCF_003129905.1	s__Bifidobacterium callitrichidarum	83.129	475	756	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002259745.1	s__Bifidobacterium myosotis	82.911	458	756	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	96.86	96.74	0.90	0.89	3	-
GCF_000741695.1	s__Bifidobacterium reuteri	82.8497	435	756	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	98.43	97.71	0.91	0.87	9	-
GCF_009193305.1	s__Bifidobacterium cebidarum	82.6901	428	756	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_018555455.1	s__Bifidobacterium sp018555455	82.5197	435	756	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_012932375.1	s__Bifidobacterium sp012932375	82.2514	422	756	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_012932425.1	s__Bifidobacterium sp012932425	81.1666	380	756	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Bifidobacteriaceae;g__Bifidobacterium	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 12:47:42,029] [INFO] GTDB search result was written to GCF_001025175.1_ASM102517v1_genomic.fna/result_gtdb.tsv
[2024-01-24 12:47:42,029] [INFO] ===== GTDB Search completed =====
[2024-01-24 12:47:42,033] [INFO] DFAST_QC result json was written to GCF_001025175.1_ASM102517v1_genomic.fna/dqc_result.json
[2024-01-24 12:47:42,033] [INFO] DFAST_QC completed!
[2024-01-24 12:47:42,033] [INFO] Total running time: 0h0m53s