[2024-01-25 20:22:50,556] [INFO] DFAST_QC pipeline started.
[2024-01-25 20:22:50,558] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 20:22:50,558] [INFO] DQC Reference Directory: /var/lib/cwl/stgf095f1b9-8f3c-4785-bb69-ed3d67c4b194/dqc_reference
[2024-01-25 20:22:51,685] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 20:22:51,686] [INFO] Task started: Prodigal
[2024-01-25 20:22:51,686] [INFO] Running command: gunzip -c /var/lib/cwl/stg3f951416-8daf-4314-8ba3-9a209a89456e/GCF_001435155.1_ASM143515v1_genomic.fna.gz | prodigal -d GCF_001435155.1_ASM143515v1_genomic.fna/cds.fna -a GCF_001435155.1_ASM143515v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 20:22:57,998] [INFO] Task succeeded: Prodigal
[2024-01-25 20:22:57,998] [INFO] Task started: HMMsearch
[2024-01-25 20:22:57,998] [INFO] Running command: hmmsearch --tblout GCF_001435155.1_ASM143515v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgf095f1b9-8f3c-4785-bb69-ed3d67c4b194/dqc_reference/reference_markers.hmm GCF_001435155.1_ASM143515v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 20:22:58,195] [INFO] Task succeeded: HMMsearch
[2024-01-25 20:22:58,196] [INFO] Found 6/6 markers.
[2024-01-25 20:22:58,216] [INFO] Query marker FASTA was written to GCF_001435155.1_ASM143515v1_genomic.fna/markers.fasta
[2024-01-25 20:22:58,216] [INFO] Task started: Blastn
[2024-01-25 20:22:58,216] [INFO] Running command: blastn -query GCF_001435155.1_ASM143515v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgf095f1b9-8f3c-4785-bb69-ed3d67c4b194/dqc_reference/reference_markers.fasta -out GCF_001435155.1_ASM143515v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 20:22:58,752] [INFO] Task succeeded: Blastn
[2024-01-25 20:22:58,755] [INFO] Selected 12 target genomes.
[2024-01-25 20:22:58,755] [INFO] Target genome list was writen to GCF_001435155.1_ASM143515v1_genomic.fna/target_genomes.txt
[2024-01-25 20:22:58,763] [INFO] Task started: fastANI
[2024-01-25 20:22:58,764] [INFO] Running command: fastANI --query /var/lib/cwl/stg3f951416-8daf-4314-8ba3-9a209a89456e/GCF_001435155.1_ASM143515v1_genomic.fna.gz --refList GCF_001435155.1_ASM143515v1_genomic.fna/target_genomes.txt --output GCF_001435155.1_ASM143515v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 20:23:05,673] [INFO] Task succeeded: fastANI
[2024-01-25 20:23:05,673] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgf095f1b9-8f3c-4785-bb69-ed3d67c4b194/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 20:23:05,674] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgf095f1b9-8f3c-4785-bb69-ed3d67c4b194/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 20:23:05,679] [INFO] Found 7 fastANI hits (3 hits with ANI > threshold)
[2024-01-25 20:23:05,680] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-25 20:23:05,680] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Lacticaseibacillus pantheris	strain=DSM 15945	GCA_001435155.1	171523	171523	type	True	100.0	818	820	95	conclusive
Lacticaseibacillus pantheris	strain=JCM 12539	GCA_001311175.1	171523	171523	type	True	99.8498	787	820	95	conclusive
Lacticaseibacillus pantheris	strain=NBRC 106106	GCA_001591865.1	171523	171523	type	True	99.7643	711	820	95	conclusive
Lacticaseibacillus thailandensis	strain=DSM 22698	GCA_001436135.1	381741	381741	type	True	80.533	362	820	95	below_threshold
Lacticaseibacillus thailandensis	strain=JCM 13996	GCA_001312865.1	381741	381741	type	True	80.4606	370	820	95	below_threshold
Lacticaseibacillus sharpeae	strain=DSM 20505	GCA_001436225.1	1626	1626	type	True	77.8764	100	820	95	below_threshold
Lacticaseibacillus sharpeae	strain=JCM 1186	GCA_001311055.1	1626	1626	type	True	77.8497	99	820	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 20:23:05,681] [INFO] DFAST Taxonomy check result was written to GCF_001435155.1_ASM143515v1_genomic.fna/tc_result.tsv
[2024-01-25 20:23:05,682] [INFO] ===== Taxonomy check completed =====
[2024-01-25 20:23:05,682] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 20:23:05,682] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgf095f1b9-8f3c-4785-bb69-ed3d67c4b194/dqc_reference/checkm_data
[2024-01-25 20:23:05,683] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 20:23:05,710] [INFO] Task started: CheckM
[2024-01-25 20:23:05,710] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_001435155.1_ASM143515v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_001435155.1_ASM143515v1_genomic.fna/checkm_input GCF_001435155.1_ASM143515v1_genomic.fna/checkm_result
[2024-01-25 20:23:29,115] [INFO] Task succeeded: CheckM
[2024-01-25 20:23:29,116] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 20:23:29,134] [INFO] ===== Completeness check finished =====
[2024-01-25 20:23:29,135] [INFO] ===== Start GTDB Search =====
[2024-01-25 20:23:29,135] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_001435155.1_ASM143515v1_genomic.fna/markers.fasta)
[2024-01-25 20:23:29,135] [INFO] Task started: Blastn
[2024-01-25 20:23:29,135] [INFO] Running command: blastn -query GCF_001435155.1_ASM143515v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgf095f1b9-8f3c-4785-bb69-ed3d67c4b194/dqc_reference/reference_markers_gtdb.fasta -out GCF_001435155.1_ASM143515v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 20:23:29,869] [INFO] Task succeeded: Blastn
[2024-01-25 20:23:29,872] [INFO] Selected 15 target genomes.
[2024-01-25 20:23:29,872] [INFO] Target genome list was writen to GCF_001435155.1_ASM143515v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 20:23:29,903] [INFO] Task started: fastANI
[2024-01-25 20:23:29,903] [INFO] Running command: fastANI --query /var/lib/cwl/stg3f951416-8daf-4314-8ba3-9a209a89456e/GCF_001435155.1_ASM143515v1_genomic.fna.gz --refList GCF_001435155.1_ASM143515v1_genomic.fna/target_genomes_gtdb.txt --output GCF_001435155.1_ASM143515v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 20:23:38,265] [INFO] Task succeeded: fastANI
[2024-01-25 20:23:38,271] [INFO] Found 7 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 20:23:38,271] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_001311175.1	s__Lacticaseibacillus pantheris	99.8498	787	820	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lacticaseibacillus	95.0	99.89	99.89	0.99	0.98	3	conclusive
GCF_001312865.1	s__Lacticaseibacillus thailandensis	80.4606	370	820	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lacticaseibacillus	95.0	99.89	99.89	0.99	0.99	2	-
GCF_004010095.1	s__Lacticaseibacillus hulanensis	78.4218	106	820	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lacticaseibacillus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001436225.1	s__Lacticaseibacillus sharpeae	77.8764	100	820	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lacticaseibacillus	95.0	99.95	99.95	0.98	0.98	2	-
GCF_003946025.1	s__Lacticaseibacillus songhuajiangensis	77.7643	106	820	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lacticaseibacillus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003946115.1	s__Lacticaseibacillus daqingensis	77.5001	50	820	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lacticaseibacillus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_009687905.1	s__Lacticaseibacillus zhaodongensis	77.3724	97	820	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lacticaseibacillus	95.0	99.99	99.99	1.00	1.00	2	-
--------------------------------------------------------------------------------
[2024-01-25 20:23:38,272] [INFO] GTDB search result was written to GCF_001435155.1_ASM143515v1_genomic.fna/result_gtdb.tsv
[2024-01-25 20:23:38,273] [INFO] ===== GTDB Search completed =====
[2024-01-25 20:23:38,275] [INFO] DFAST_QC result json was written to GCF_001435155.1_ASM143515v1_genomic.fna/dqc_result.json
[2024-01-25 20:23:38,275] [INFO] DFAST_QC completed!
[2024-01-25 20:23:38,275] [INFO] Total running time: 0h0m48s