[2024-01-24 10:57:13,142] [INFO] DFAST_QC pipeline started.
[2024-01-24 10:57:13,144] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 10:57:13,144] [INFO] DQC Reference Directory: /var/lib/cwl/stgd371c291-f848-485f-9c7b-1a5b765f7753/dqc_reference
[2024-01-24 10:57:14,552] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 10:57:14,553] [INFO] Task started: Prodigal
[2024-01-24 10:57:14,554] [INFO] Running command: gunzip -c /var/lib/cwl/stg33b7f7d9-6738-4f72-810e-693acd6849a5/GCF_001481685.1_ASM148168v1_genomic.fna.gz | prodigal -d GCF_001481685.1_ASM148168v1_genomic.fna/cds.fna -a GCF_001481685.1_ASM148168v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 10:57:17,793] [INFO] Task succeeded: Prodigal
[2024-01-24 10:57:17,793] [INFO] Task started: HMMsearch
[2024-01-24 10:57:17,794] [INFO] Running command: hmmsearch --tblout GCF_001481685.1_ASM148168v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgd371c291-f848-485f-9c7b-1a5b765f7753/dqc_reference/reference_markers.hmm GCF_001481685.1_ASM148168v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 10:57:18,008] [INFO] Task succeeded: HMMsearch
[2024-01-24 10:57:18,009] [WARNING] Found 2/6 markers. [/var/lib/cwl/stg33b7f7d9-6738-4f72-810e-693acd6849a5/GCF_001481685.1_ASM148168v1_genomic.fna.gz]
[2024-01-24 10:57:18,037] [INFO] Query marker FASTA was written to GCF_001481685.1_ASM148168v1_genomic.fna/markers.fasta
[2024-01-24 10:57:18,038] [INFO] Task started: Blastn
[2024-01-24 10:57:18,038] [INFO] Running command: blastn -query GCF_001481685.1_ASM148168v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgd371c291-f848-485f-9c7b-1a5b765f7753/dqc_reference/reference_markers.fasta -out GCF_001481685.1_ASM148168v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 10:57:18,547] [INFO] Task succeeded: Blastn
[2024-01-24 10:57:18,549] [INFO] Selected 1 target genomes.
[2024-01-24 10:57:18,549] [INFO] Target genome list was writen to GCF_001481685.1_ASM148168v1_genomic.fna/target_genomes.txt
[2024-01-24 10:57:18,565] [INFO] Task started: fastANI
[2024-01-24 10:57:18,565] [INFO] Running command: fastANI --query /var/lib/cwl/stg33b7f7d9-6738-4f72-810e-693acd6849a5/GCF_001481685.1_ASM148168v1_genomic.fna.gz --refList GCF_001481685.1_ASM148168v1_genomic.fna/target_genomes.txt --output GCF_001481685.1_ASM148168v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 10:57:19,164] [INFO] Task succeeded: fastANI
[2024-01-24 10:57:19,164] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgd371c291-f848-485f-9c7b-1a5b765f7753/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 10:57:19,164] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgd371c291-f848-485f-9c7b-1a5b765f7753/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 10:57:19,195] [INFO] Found 1 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 10:57:19,195] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 10:57:19,195] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Ignicoccus islandicus	strain=DSM 13165	GCA_001481685.1	54259	54259	type	True	100.0	464	464	95	conclusive
--------------------------------------------------------------------------------
[2024-01-24 10:57:19,197] [INFO] DFAST Taxonomy check result was written to GCF_001481685.1_ASM148168v1_genomic.fna/tc_result.tsv
[2024-01-24 10:57:19,197] [INFO] ===== Taxonomy check completed =====
[2024-01-24 10:57:19,197] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 10:57:19,197] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgd371c291-f848-485f-9c7b-1a5b765f7753/dqc_reference/checkm_data
[2024-01-24 10:57:19,203] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 10:57:19,219] [INFO] Task started: CheckM
[2024-01-24 10:57:19,220] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_001481685.1_ASM148168v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_001481685.1_ASM148168v1_genomic.fna/checkm_input GCF_001481685.1_ASM148168v1_genomic.fna/checkm_result
[2024-01-24 10:57:41,642] [INFO] Task succeeded: CheckM
[2024-01-24 10:57:41,644] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 91.67%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 10:57:41,658] [INFO] ===== Completeness check finished =====
[2024-01-24 10:57:41,658] [INFO] ===== Start GTDB Search =====
[2024-01-24 10:57:41,659] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_001481685.1_ASM148168v1_genomic.fna/markers.fasta)
[2024-01-24 10:57:41,660] [INFO] Task started: Blastn
[2024-01-24 10:57:41,660] [INFO] Running command: blastn -query GCF_001481685.1_ASM148168v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgd371c291-f848-485f-9c7b-1a5b765f7753/dqc_reference/reference_markers_gtdb.fasta -out GCF_001481685.1_ASM148168v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 10:57:42,636] [INFO] Task succeeded: Blastn
[2024-01-24 10:57:42,639] [INFO] Selected 2 target genomes.
[2024-01-24 10:57:42,639] [INFO] Target genome list was writen to GCF_001481685.1_ASM148168v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 10:57:42,643] [INFO] Task started: fastANI
[2024-01-24 10:57:42,643] [INFO] Running command: fastANI --query /var/lib/cwl/stg33b7f7d9-6738-4f72-810e-693acd6849a5/GCF_001481685.1_ASM148168v1_genomic.fna.gz --refList GCF_001481685.1_ASM148168v1_genomic.fna/target_genomes_gtdb.txt --output GCF_001481685.1_ASM148168v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 10:57:43,501] [INFO] Task succeeded: fastANI
[2024-01-24 10:57:43,513] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 10:57:43,513] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_001481685.1	s__Ignicoccus islandicus	100.0	464	464	d__Archaea;p__Thermoproteota;c__Thermoproteia;o__Sulfolobales;f__Ignicoccaceae;g__Ignicoccus	95.0	N/A	N/A	N/A	N/A	1	conclusive
--------------------------------------------------------------------------------
[2024-01-24 10:57:43,518] [INFO] GTDB search result was written to GCF_001481685.1_ASM148168v1_genomic.fna/result_gtdb.tsv
[2024-01-24 10:57:43,519] [INFO] ===== GTDB Search completed =====
[2024-01-24 10:57:43,522] [INFO] DFAST_QC result json was written to GCF_001481685.1_ASM148168v1_genomic.fna/dqc_result.json
[2024-01-24 10:57:43,523] [INFO] DFAST_QC completed!
[2024-01-24 10:57:43,523] [INFO] Total running time: 0h0m30s