[2024-01-24 12:06:12,199] [INFO] DFAST_QC pipeline started.
[2024-01-24 12:06:12,205] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 12:06:12,205] [INFO] DQC Reference Directory: /var/lib/cwl/stg9e17ab8f-e404-4a04-b4d2-6e5f511dc8c6/dqc_reference
[2024-01-24 12:06:13,511] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 12:06:13,512] [INFO] Task started: Prodigal
[2024-01-24 12:06:13,512] [INFO] Running command: gunzip -c /var/lib/cwl/stg9908dd97-7a5c-45b1-9196-779c79f22a87/GCF_001571065.1_ASM157106v1_genomic.fna.gz | prodigal -d GCF_001571065.1_ASM157106v1_genomic.fna/cds.fna -a GCF_001571065.1_ASM157106v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 12:06:23,515] [INFO] Task succeeded: Prodigal
[2024-01-24 12:06:23,515] [INFO] Task started: HMMsearch
[2024-01-24 12:06:23,516] [INFO] Running command: hmmsearch --tblout GCF_001571065.1_ASM157106v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg9e17ab8f-e404-4a04-b4d2-6e5f511dc8c6/dqc_reference/reference_markers.hmm GCF_001571065.1_ASM157106v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 12:06:23,802] [INFO] Task succeeded: HMMsearch
[2024-01-24 12:06:23,803] [INFO] Found 6/6 markers.
[2024-01-24 12:06:23,839] [INFO] Query marker FASTA was written to GCF_001571065.1_ASM157106v1_genomic.fna/markers.fasta
[2024-01-24 12:06:23,839] [INFO] Task started: Blastn
[2024-01-24 12:06:23,840] [INFO] Running command: blastn -query GCF_001571065.1_ASM157106v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg9e17ab8f-e404-4a04-b4d2-6e5f511dc8c6/dqc_reference/reference_markers.fasta -out GCF_001571065.1_ASM157106v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:06:24,978] [INFO] Task succeeded: Blastn
[2024-01-24 12:06:24,981] [INFO] Selected 8 target genomes.
[2024-01-24 12:06:24,982] [INFO] Target genome list was writen to GCF_001571065.1_ASM157106v1_genomic.fna/target_genomes.txt
[2024-01-24 12:06:24,987] [INFO] Task started: fastANI
[2024-01-24 12:06:24,987] [INFO] Running command: fastANI --query /var/lib/cwl/stg9908dd97-7a5c-45b1-9196-779c79f22a87/GCF_001571065.1_ASM157106v1_genomic.fna.gz --refList GCF_001571065.1_ASM157106v1_genomic.fna/target_genomes.txt --output GCF_001571065.1_ASM157106v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 12:06:31,294] [INFO] Task succeeded: fastANI
[2024-01-24 12:06:31,295] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg9e17ab8f-e404-4a04-b4d2-6e5f511dc8c6/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 12:06:31,295] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg9e17ab8f-e404-4a04-b4d2-6e5f511dc8c6/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 12:06:31,303] [INFO] Found 8 fastANI hits (2 hits with ANI > threshold)
[2024-01-24 12:06:31,303] [INFO] The taxonomy check result is classified as 'inconclusive'.
[2024-01-24 12:06:31,303] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Dietzia cinnamea	strain=NBRC 102147	GCA_001571065.1	321318	321318	type	True	100.0	1120	1124	95	inconclusive
Dietzia massiliensis	strain=Marseille-Q0999	GCA_018390595.1	2697499	2697499	type	True	95.4127	928	1124	95	inconclusive
Dietzia lutea	strain=YIM 80766	GCA_003096075.1	546160	546160	type	True	93.4454	967	1124	95	below_threshold
Dietzia lutea	strain=YIM 80766	GCA_009806405.1	546160	546160	type	True	92.6669	543	1124	95	below_threshold
Dietzia kunjamensis	strain=DSM 44907	GCA_003610395.1	322509	322509	type	True	86.3673	911	1124	95	below_threshold
Dietzia kunjamensis subsp. schimae	strain=DSM 45139	GCA_900182685.1	498198	322509	type	True	86.3486	934	1124	95	below_threshold
Dietzia kunjamensis	strain=K30-10T	GCA_014144965.1	322509	322509	type	True	86.3048	615	1124	95	below_threshold
Dietzia aerolata	strain=Sj14aT	GCA_014144865.1	595984	595984	type	True	82.9348	625	1124	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 12:06:31,305] [INFO] DFAST Taxonomy check result was written to GCF_001571065.1_ASM157106v1_genomic.fna/tc_result.tsv
[2024-01-24 12:06:31,306] [INFO] ===== Taxonomy check completed =====
[2024-01-24 12:06:31,306] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 12:06:31,306] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg9e17ab8f-e404-4a04-b4d2-6e5f511dc8c6/dqc_reference/checkm_data
[2024-01-24 12:06:31,307] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 12:06:31,346] [INFO] Task started: CheckM
[2024-01-24 12:06:31,346] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_001571065.1_ASM157106v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_001571065.1_ASM157106v1_genomic.fna/checkm_input GCF_001571065.1_ASM157106v1_genomic.fna/checkm_result
[2024-01-24 12:07:07,650] [INFO] Task succeeded: CheckM
[2024-01-24 12:07:07,651] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 12:07:07,678] [INFO] ===== Completeness check finished =====
[2024-01-24 12:07:07,678] [INFO] ===== Start GTDB Search =====
[2024-01-24 12:07:07,679] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_001571065.1_ASM157106v1_genomic.fna/markers.fasta)
[2024-01-24 12:07:07,679] [INFO] Task started: Blastn
[2024-01-24 12:07:07,679] [INFO] Running command: blastn -query GCF_001571065.1_ASM157106v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg9e17ab8f-e404-4a04-b4d2-6e5f511dc8c6/dqc_reference/reference_markers_gtdb.fasta -out GCF_001571065.1_ASM157106v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:07:09,480] [INFO] Task succeeded: Blastn
[2024-01-24 12:07:09,485] [INFO] Selected 8 target genomes.
[2024-01-24 12:07:09,485] [INFO] Target genome list was writen to GCF_001571065.1_ASM157106v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 12:07:09,491] [INFO] Task started: fastANI
[2024-01-24 12:07:09,492] [INFO] Running command: fastANI --query /var/lib/cwl/stg9908dd97-7a5c-45b1-9196-779c79f22a87/GCF_001571065.1_ASM157106v1_genomic.fna.gz --refList GCF_001571065.1_ASM157106v1_genomic.fna/target_genomes_gtdb.txt --output GCF_001571065.1_ASM157106v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 12:07:15,734] [INFO] Task succeeded: fastANI
[2024-01-24 12:07:15,741] [INFO] Found 8 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 12:07:15,742] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_001571065.1	s__Dietzia cinnamea	100.0	1120	1124	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Dietzia	95.1735	97.27	96.55	0.88	0.84	8	conclusive
GCF_000186325.1	s__Dietzia cinnamea_A	95.0767	842	1124	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Dietzia	95.1735	96.59	96.10	0.87	0.83	4	-
GCF_003096075.1	s__Dietzia lutea	93.4651	966	1124	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Dietzia	95.0	99.99	99.99	1.00	1.00	2	-
GCF_014144855.1	s__Dietzia maris	86.3697	571	1124	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Dietzia	95.0	97.49	96.42	0.87	0.74	16	-
GCF_008369165.1	s__Dietzia sp008369165	83.8424	801	1124	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Dietzia	95.0	N/A	N/A	N/A	N/A	1	-
GCA_012841845.1	s__Dietzia sp012841845	83.3784	453	1124	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Dietzia	95.0	N/A	N/A	N/A	N/A	1	-
GCF_014144865.1	s__Dietzia aerolata	82.8986	628	1124	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Dietzia	95.0	97.62	97.62	0.76	0.76	2	-
GCF_000226215.1	s__Dietzia alimentaria	81.9058	732	1124	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Dietzia	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 12:07:15,745] [INFO] GTDB search result was written to GCF_001571065.1_ASM157106v1_genomic.fna/result_gtdb.tsv
[2024-01-24 12:07:15,746] [INFO] ===== GTDB Search completed =====
[2024-01-24 12:07:15,751] [INFO] DFAST_QC result json was written to GCF_001571065.1_ASM157106v1_genomic.fna/dqc_result.json
[2024-01-24 12:07:15,751] [INFO] DFAST_QC completed!
[2024-01-24 12:07:15,751] [INFO] Total running time: 0h1m4s