[2024-01-25 18:56:05,718] [INFO] DFAST_QC pipeline started.
[2024-01-25 18:56:05,721] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 18:56:05,721] [INFO] DQC Reference Directory: /var/lib/cwl/stge58a7ec5-ba90-4548-a4c4-64e3c2249889/dqc_reference
[2024-01-25 18:56:06,886] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 18:56:06,887] [INFO] Task started: Prodigal
[2024-01-25 18:56:06,887] [INFO] Running command: gunzip -c /var/lib/cwl/stg0c35fe51-c0df-48dd-b6ef-3a7a4e9635bf/GCF_002003265.1_ASM200326v1_genomic.fna.gz | prodigal -d GCF_002003265.1_ASM200326v1_genomic.fna/cds.fna -a GCF_002003265.1_ASM200326v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 18:56:16,300] [INFO] Task succeeded: Prodigal
[2024-01-25 18:56:16,300] [INFO] Task started: HMMsearch
[2024-01-25 18:56:16,300] [INFO] Running command: hmmsearch --tblout GCF_002003265.1_ASM200326v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stge58a7ec5-ba90-4548-a4c4-64e3c2249889/dqc_reference/reference_markers.hmm GCF_002003265.1_ASM200326v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 18:56:16,549] [INFO] Task succeeded: HMMsearch
[2024-01-25 18:56:16,550] [INFO] Found 6/6 markers.
[2024-01-25 18:56:16,587] [INFO] Query marker FASTA was written to GCF_002003265.1_ASM200326v1_genomic.fna/markers.fasta
[2024-01-25 18:56:16,588] [INFO] Task started: Blastn
[2024-01-25 18:56:16,588] [INFO] Running command: blastn -query GCF_002003265.1_ASM200326v1_genomic.fna/markers.fasta -db /var/lib/cwl/stge58a7ec5-ba90-4548-a4c4-64e3c2249889/dqc_reference/reference_markers.fasta -out GCF_002003265.1_ASM200326v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 18:56:17,123] [INFO] Task succeeded: Blastn
[2024-01-25 18:56:17,128] [INFO] Selected 21 target genomes.
[2024-01-25 18:56:17,128] [INFO] Target genome list was writen to GCF_002003265.1_ASM200326v1_genomic.fna/target_genomes.txt
[2024-01-25 18:56:17,140] [INFO] Task started: fastANI
[2024-01-25 18:56:17,141] [INFO] Running command: fastANI --query /var/lib/cwl/stg0c35fe51-c0df-48dd-b6ef-3a7a4e9635bf/GCF_002003265.1_ASM200326v1_genomic.fna.gz --refList GCF_002003265.1_ASM200326v1_genomic.fna/target_genomes.txt --output GCF_002003265.1_ASM200326v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 18:56:38,112] [INFO] Task succeeded: fastANI
[2024-01-25 18:56:38,113] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stge58a7ec5-ba90-4548-a4c4-64e3c2249889/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 18:56:38,113] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stge58a7ec5-ba90-4548-a4c4-64e3c2249889/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 18:56:38,119] [INFO] Found 7 fastANI hits (2 hits with ANI > threshold)
[2024-01-25 18:56:38,120] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-25 18:56:38,120] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Paenibacillus larvae subsp. larvae	strain=ATCC 9545	GCA_002003265.1	147375	1464	type	True	100.0	1423	1429	95	conclusive
Paenibacillus larvae subsp. larvae	strain=ERIC_I	GCA_002951875.1	147375	1464	type	True	99.9748	1416	1429	95	conclusive
Paenibacillus curdlanolyticus	strain=YK9	GCA_000179615.1	59840	59840	type	True	80.5901	50	1429	95	below_threshold
Paenibacillus uliginis	strain=N3/975	GCA_900177425.1	683737	683737	type	True	77.869	57	1429	95	below_threshold
Paenibacillus anseongense	strain=MAH-34	GCA_009757995.1	2682845	2682845	type	True	77.1523	70	1429	95	below_threshold
Paenibacillus rigui	strain=JCM 16352	GCA_002234615.1	554312	554312	type	True	76.9453	67	1429	95	below_threshold
Paenibacillus eucommiae	strain=DSM 26048	GCA_017874215.1	1355755	1355755	type	True	76.5601	67	1429	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 18:56:38,121] [INFO] DFAST Taxonomy check result was written to GCF_002003265.1_ASM200326v1_genomic.fna/tc_result.tsv
[2024-01-25 18:56:38,122] [INFO] ===== Taxonomy check completed =====
[2024-01-25 18:56:38,122] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 18:56:38,122] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stge58a7ec5-ba90-4548-a4c4-64e3c2249889/dqc_reference/checkm_data
[2024-01-25 18:56:38,123] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 18:56:38,166] [INFO] Task started: CheckM
[2024-01-25 18:56:38,166] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_002003265.1_ASM200326v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_002003265.1_ASM200326v1_genomic.fna/checkm_input GCF_002003265.1_ASM200326v1_genomic.fna/checkm_result
[2024-01-25 18:57:09,361] [INFO] Task succeeded: CheckM
[2024-01-25 18:57:09,362] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 18:57:09,393] [INFO] ===== Completeness check finished =====
[2024-01-25 18:57:09,393] [INFO] ===== Start GTDB Search =====
[2024-01-25 18:57:09,394] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_002003265.1_ASM200326v1_genomic.fna/markers.fasta)
[2024-01-25 18:57:09,394] [INFO] Task started: Blastn
[2024-01-25 18:57:09,394] [INFO] Running command: blastn -query GCF_002003265.1_ASM200326v1_genomic.fna/markers.fasta -db /var/lib/cwl/stge58a7ec5-ba90-4548-a4c4-64e3c2249889/dqc_reference/reference_markers_gtdb.fasta -out GCF_002003265.1_ASM200326v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 18:57:10,113] [INFO] Task succeeded: Blastn
[2024-01-25 18:57:10,116] [INFO] Selected 26 target genomes.
[2024-01-25 18:57:10,116] [INFO] Target genome list was writen to GCF_002003265.1_ASM200326v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 18:57:10,141] [INFO] Task started: fastANI
[2024-01-25 18:57:10,141] [INFO] Running command: fastANI --query /var/lib/cwl/stg0c35fe51-c0df-48dd-b6ef-3a7a4e9635bf/GCF_002003265.1_ASM200326v1_genomic.fna.gz --refList GCF_002003265.1_ASM200326v1_genomic.fna/target_genomes_gtdb.txt --output GCF_002003265.1_ASM200326v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 18:57:39,748] [INFO] Task succeeded: fastANI
[2024-01-25 18:57:39,756] [INFO] Found 12 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 18:57:39,757] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_002003265.1	s__Paenibacillus_H larvae	100.0	1426	1429	d__Bacteria;p__Firmicutes;c__Bacilli;o__Paenibacillales;f__NBRC-103111;g__Paenibacillus_H	95.0	99.26	98.58	0.93	0.88	15	conclusive
GCF_000179615.1	s__Paenibacillus_T curdlanolyticus	81.2352	53	1429	d__Bacteria;p__Firmicutes;c__Bacilli;o__Paenibacillales;f__Paenibacillaceae;g__Paenibacillus_T	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000961095.1	s__Paenibacillus_O beijingensis	80.375	50	1429	d__Bacteria;p__Firmicutes;c__Bacilli;o__Paenibacillales;f__Paenibacillaceae;g__Paenibacillus_O	95.0	N/A	N/A	N/A	N/A	1	-
GCF_013401555.1	s__Paenibacillus sp900110055	79.6465	51	1429	d__Bacteria;p__Firmicutes;c__Bacilli;o__Paenibacillales;f__Paenibacillaceae;g__Paenibacillus	95.0	97.27	96.55	0.92	0.89	5	-
GCF_900177425.1	s__Paenibacillus uliginis	77.8691	57	1429	d__Bacteria;p__Firmicutes;c__Bacilli;o__Paenibacillales;f__Paenibacillaceae;g__Paenibacillus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001955535.1	s__Paenibacillus sp001955535	77.3516	54	1429	d__Bacteria;p__Firmicutes;c__Bacilli;o__Paenibacillales;f__Paenibacillaceae;g__Paenibacillus	95.0	98.62	98.62	0.85	0.85	2	-
GCF_013204845.1	s__Paenibacillus_E qinlingensis	77.243	64	1429	d__Bacteria;p__Firmicutes;c__Bacilli;o__Paenibacillales;f__NBRC-103111;g__Paenibacillus_E	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001428105.1	s__Paenibacillus_E sp001428105	77.0385	57	1429	d__Bacteria;p__Firmicutes;c__Bacilli;o__Paenibacillales;f__NBRC-103111;g__Paenibacillus_E	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003955665.1	s__Paenibacillus_E whitsoniae	76.988	64	1429	d__Bacteria;p__Firmicutes;c__Bacilli;o__Paenibacillales;f__NBRC-103111;g__Paenibacillus_E	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002234615.1	s__Paenibacillus_S rigui	76.9841	66	1429	d__Bacteria;p__Firmicutes;c__Bacilli;o__Paenibacillales;f__NBRC-103111;g__Paenibacillus_S	95.0	N/A	N/A	N/A	N/A	1	-
GCF_017874215.1	s__Paenibacillus_AI eucommiae	76.5316	68	1429	d__Bacteria;p__Firmicutes;c__Bacilli;o__Paenibacillales;f__NBRC-103111;g__Paenibacillus_AI	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000686845.1	s__Paenibacillus_S sp000686845	76.5077	67	1429	d__Bacteria;p__Firmicutes;c__Bacilli;o__Paenibacillales;f__NBRC-103111;g__Paenibacillus_S	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-25 18:57:39,759] [INFO] GTDB search result was written to GCF_002003265.1_ASM200326v1_genomic.fna/result_gtdb.tsv
[2024-01-25 18:57:39,760] [INFO] ===== GTDB Search completed =====
[2024-01-25 18:57:39,764] [INFO] DFAST_QC result json was written to GCF_002003265.1_ASM200326v1_genomic.fna/dqc_result.json
[2024-01-25 18:57:39,764] [INFO] DFAST_QC completed!
[2024-01-25 18:57:39,764] [INFO] Total running time: 0h1m34s