[2024-01-25 19:30:36,686] [INFO] DFAST_QC pipeline started.
[2024-01-25 19:30:36,687] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 19:30:36,687] [INFO] DQC Reference Directory: /var/lib/cwl/stg92fa31f9-ee0d-41f7-86f5-66bdd08c7ad3/dqc_reference
[2024-01-25 19:30:37,853] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 19:30:37,854] [INFO] Task started: Prodigal
[2024-01-25 19:30:37,854] [INFO] Running command: gunzip -c /var/lib/cwl/stg83ab84dd-f4b3-4d4b-80ee-72fcd4dc8f19/GCF_002015175.1_ASM201517v1_genomic.fna.gz | prodigal -d GCF_002015175.1_ASM201517v1_genomic.fna/cds.fna -a GCF_002015175.1_ASM201517v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 19:30:43,820] [INFO] Task succeeded: Prodigal
[2024-01-25 19:30:43,820] [INFO] Task started: HMMsearch
[2024-01-25 19:30:43,820] [INFO] Running command: hmmsearch --tblout GCF_002015175.1_ASM201517v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg92fa31f9-ee0d-41f7-86f5-66bdd08c7ad3/dqc_reference/reference_markers.hmm GCF_002015175.1_ASM201517v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 19:30:43,994] [INFO] Task succeeded: HMMsearch
[2024-01-25 19:30:43,995] [INFO] Found 6/6 markers.
[2024-01-25 19:30:44,015] [INFO] Query marker FASTA was written to GCF_002015175.1_ASM201517v1_genomic.fna/markers.fasta
[2024-01-25 19:30:44,015] [INFO] Task started: Blastn
[2024-01-25 19:30:44,015] [INFO] Running command: blastn -query GCF_002015175.1_ASM201517v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg92fa31f9-ee0d-41f7-86f5-66bdd08c7ad3/dqc_reference/reference_markers.fasta -out GCF_002015175.1_ASM201517v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 19:30:44,601] [INFO] Task succeeded: Blastn
[2024-01-25 19:30:44,605] [INFO] Selected 15 target genomes.
[2024-01-25 19:30:44,605] [INFO] Target genome list was writen to GCF_002015175.1_ASM201517v1_genomic.fna/target_genomes.txt
[2024-01-25 19:30:44,937] [INFO] Task started: fastANI
[2024-01-25 19:30:44,937] [INFO] Running command: fastANI --query /var/lib/cwl/stg83ab84dd-f4b3-4d4b-80ee-72fcd4dc8f19/GCF_002015175.1_ASM201517v1_genomic.fna.gz --refList GCF_002015175.1_ASM201517v1_genomic.fna/target_genomes.txt --output GCF_002015175.1_ASM201517v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 19:30:51,933] [INFO] Task succeeded: fastANI
[2024-01-25 19:30:51,933] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg92fa31f9-ee0d-41f7-86f5-66bdd08c7ad3/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 19:30:51,934] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg92fa31f9-ee0d-41f7-86f5-66bdd08c7ad3/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 19:30:51,942] [INFO] Found 11 fastANI hits (3 hits with ANI > threshold)
[2024-01-25 19:30:51,942] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-25 19:30:51,942] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Moraxella cuniculi	strain=CCUG 2154	GCA_002015175.1	34061	34061	type	True	100.0	737	738	95	conclusive
Moraxella cuniculi	strain=DSM 21768	GCA_900156515.1	34061	34061	type	True	99.9931	729	738	95	conclusive
Moraxella cuniculi	strain=NCTC10297	GCA_900637105.1	34061	34061	type	True	98.2396	630	738	95	conclusive
Moraxella porci	strain=CCUG 54912	GCA_002014855.1	1288392	1288392	type	True	79.1788	292	738	95	below_threshold
Moraxella pluranimalium	strain=CCUG 54913	GCA_002014825.1	470453	470453	type	True	78.9196	289	738	95	below_threshold
Moraxella nasovis	strain=ZY201115	GCA_022701215.1	2904121	2904121	type	True	78.0083	140	738	95	below_threshold
Moraxella catarrhalis	strain=CCUG 353	GCA_001632285.1	480	480	type	True	77.9977	234	738	95	below_threshold
Moraxella equi	strain=CCUG 4950	GCA_002027555.1	60442	60442	type	True	77.5079	179	738	95	below_threshold
Moraxella equi	strain=NCTC11012	GCA_900453335.1	60442	60442	type	True	77.4947	180	738	95	below_threshold
Moraxella oblonga	strain=NBRC 102422	GCA_001598275.1	200413	200413	type	True	77.4352	147	738	95	below_threshold
Psychrobacter celer	strain=DSM 23510	GCA_016107555.1	306572	306572	type	True	76.6038	55	738	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 19:30:51,949] [INFO] DFAST Taxonomy check result was written to GCF_002015175.1_ASM201517v1_genomic.fna/tc_result.tsv
[2024-01-25 19:30:51,950] [INFO] ===== Taxonomy check completed =====
[2024-01-25 19:30:51,950] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 19:30:51,950] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg92fa31f9-ee0d-41f7-86f5-66bdd08c7ad3/dqc_reference/checkm_data
[2024-01-25 19:30:51,951] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 19:30:51,978] [INFO] Task started: CheckM
[2024-01-25 19:30:51,978] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_002015175.1_ASM201517v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_002015175.1_ASM201517v1_genomic.fna/checkm_input GCF_002015175.1_ASM201517v1_genomic.fna/checkm_result
[2024-01-25 19:31:14,196] [INFO] Task succeeded: CheckM
[2024-01-25 19:31:14,197] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 19:31:14,217] [INFO] ===== Completeness check finished =====
[2024-01-25 19:31:14,217] [INFO] ===== Start GTDB Search =====
[2024-01-25 19:31:14,218] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_002015175.1_ASM201517v1_genomic.fna/markers.fasta)
[2024-01-25 19:31:14,218] [INFO] Task started: Blastn
[2024-01-25 19:31:14,218] [INFO] Running command: blastn -query GCF_002015175.1_ASM201517v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg92fa31f9-ee0d-41f7-86f5-66bdd08c7ad3/dqc_reference/reference_markers_gtdb.fasta -out GCF_002015175.1_ASM201517v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 19:31:15,168] [INFO] Task succeeded: Blastn
[2024-01-25 19:31:15,171] [INFO] Selected 15 target genomes.
[2024-01-25 19:31:15,171] [INFO] Target genome list was writen to GCF_002015175.1_ASM201517v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 19:31:15,202] [INFO] Task started: fastANI
[2024-01-25 19:31:15,202] [INFO] Running command: fastANI --query /var/lib/cwl/stg83ab84dd-f4b3-4d4b-80ee-72fcd4dc8f19/GCF_002015175.1_ASM201517v1_genomic.fna.gz --refList GCF_002015175.1_ASM201517v1_genomic.fna/target_genomes_gtdb.txt --output GCF_002015175.1_ASM201517v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 19:31:21,882] [INFO] Task succeeded: fastANI
[2024-01-25 19:31:21,890] [INFO] Found 11 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 19:31:21,890] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_900156515.1	s__Moraxella cuniculi	99.9931	729	738	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Moraxella	95.0	99.10	98.21	0.93	0.87	3	conclusive
GCF_002014855.1	s__Moraxella porci	79.1625	293	738	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Moraxella	95.0	96.21	96.21	0.92	0.92	2	-
GCF_002014825.1	s__Moraxella pluranimalium	78.9484	287	738	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Moraxella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002014965.1	s__Moraxella canis	78.4791	252	738	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Moraxella	95.0	97.14	96.86	0.93	0.92	4	-
GCF_001656295.1	s__Moraxella catarrhalis_C	78.334	243	738	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Moraxella	95.0	98.44	97.90	0.94	0.92	5	-
GCF_001632285.1	s__Moraxella catarrhalis	78.0003	232	738	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Moraxella	95.0	98.72	95.96	0.96	0.91	200	-
GCF_000696305.2	s__Moraxella bovoculi	77.7298	145	738	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Moraxella	95.978	98.49	98.41	0.96	0.96	5	-
GCF_002027555.1	s__Moraxella equi	77.5032	180	738	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Moraxella	95.0	99.99	99.99	1.00	1.00	2	-
GCF_001598275.1	s__Moraxella oblonga	77.3964	149	738	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Moraxella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002014975.1	s__Moraxella bovis	77.3872	164	738	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Moraxella	95.6695	98.79	98.38	0.94	0.91	3	-
GCF_016107555.1	s__Psychrobacter celer	76.6038	55	738	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Moraxellaceae;g__Psychrobacter	95.0	97.12	95.68	0.88	0.85	9	-
--------------------------------------------------------------------------------
[2024-01-25 19:31:21,893] [INFO] GTDB search result was written to GCF_002015175.1_ASM201517v1_genomic.fna/result_gtdb.tsv
[2024-01-25 19:31:21,893] [INFO] ===== GTDB Search completed =====
[2024-01-25 19:31:21,896] [INFO] DFAST_QC result json was written to GCF_002015175.1_ASM201517v1_genomic.fna/dqc_result.json
[2024-01-25 19:31:21,896] [INFO] DFAST_QC completed!
[2024-01-25 19:31:21,896] [INFO] Total running time: 0h0m45s