[2024-01-24 11:52:04,680] [INFO] DFAST_QC pipeline started.
[2024-01-24 11:52:04,682] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 11:52:04,682] [INFO] DQC Reference Directory: /var/lib/cwl/stg9d074dd3-2052-4f82-bd35-0106c52de381/dqc_reference
[2024-01-24 11:52:05,882] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 11:52:05,883] [INFO] Task started: Prodigal
[2024-01-24 11:52:05,883] [INFO] Running command: gunzip -c /var/lib/cwl/stg62de9ea0-09be-438e-b3cf-51d7d1096d47/GCF_002217205.1_ASM221720v1_genomic.fna.gz | prodigal -d GCF_002217205.1_ASM221720v1_genomic.fna/cds.fna -a GCF_002217205.1_ASM221720v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 11:52:19,990] [INFO] Task succeeded: Prodigal
[2024-01-24 11:52:19,991] [INFO] Task started: HMMsearch
[2024-01-24 11:52:19,991] [INFO] Running command: hmmsearch --tblout GCF_002217205.1_ASM221720v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg9d074dd3-2052-4f82-bd35-0106c52de381/dqc_reference/reference_markers.hmm GCF_002217205.1_ASM221720v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 11:52:20,274] [INFO] Task succeeded: HMMsearch
[2024-01-24 11:52:20,276] [INFO] Found 6/6 markers.
[2024-01-24 11:52:20,310] [INFO] Query marker FASTA was written to GCF_002217205.1_ASM221720v1_genomic.fna/markers.fasta
[2024-01-24 11:52:20,311] [INFO] Task started: Blastn
[2024-01-24 11:52:20,311] [INFO] Running command: blastn -query GCF_002217205.1_ASM221720v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg9d074dd3-2052-4f82-bd35-0106c52de381/dqc_reference/reference_markers.fasta -out GCF_002217205.1_ASM221720v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:52:20,950] [INFO] Task succeeded: Blastn
[2024-01-24 11:52:20,954] [INFO] Selected 19 target genomes.
[2024-01-24 11:52:20,955] [INFO] Target genome list was writen to GCF_002217205.1_ASM221720v1_genomic.fna/target_genomes.txt
[2024-01-24 11:52:20,975] [INFO] Task started: fastANI
[2024-01-24 11:52:20,975] [INFO] Running command: fastANI --query /var/lib/cwl/stg62de9ea0-09be-438e-b3cf-51d7d1096d47/GCF_002217205.1_ASM221720v1_genomic.fna.gz --refList GCF_002217205.1_ASM221720v1_genomic.fna/target_genomes.txt --output GCF_002217205.1_ASM221720v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 11:52:32,717] [INFO] Task succeeded: fastANI
[2024-01-24 11:52:32,718] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg9d074dd3-2052-4f82-bd35-0106c52de381/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 11:52:32,718] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg9d074dd3-2052-4f82-bd35-0106c52de381/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 11:52:32,736] [INFO] Found 19 fastANI hits (3 hits with ANI > threshold)
[2024-01-24 11:52:32,736] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 11:52:32,736] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Flavobacterium branchiophilum	strain=ATCC 35035	GCA_002217205.1	55197	55197	type	True	100.0	1060	1072	95	conclusive
Flavobacterium branchiophilum	strain=DSM 24789	GCA_006716585.1	55197	55197	type	True	99.9767	1070	1072	95	conclusive
Flavobacterium branchiophilum	strain=NBRC 15030	GCA_007990815.1	55197	55197	type	True	99.9427	1034	1072	95	conclusive
Flavobacterium proteolyticum	strain=1Y8A	GCA_015223105.1	2911683	2911683	type	True	77.9269	112	1072	95	below_threshold
Flavobacterium dankookense	strain=DSM 25687	GCA_004362665.1	706186	706186	type	True	77.6327	168	1072	95	below_threshold
Flavobacterium macrobrachii	strain=an-8	GCA_014486675.1	591204	591204	type	True	77.5882	157	1072	95	below_threshold
Flavobacterium crassostreae	strain=LPB0076	GCA_001831475.1	1763534	1763534	type	True	77.5598	147	1072	95	below_threshold
Flavobacterium cellulosilyticum	strain=AR-3-4	GCA_004349355.1	2541731	2541731	type	True	77.549	171	1072	95	below_threshold
Flavobacterium gawalongense	strain=GSP16	GCA_007097265.1	2594432	2594432	type	True	77.4363	171	1072	95	below_threshold
Flavobacterium luteum	strain=NBRC 112527	GCA_008806775.1	2026654	2026654	type	True	77.4299	186	1072	95	below_threshold
Flavobacterium ichthyis	strain=NST-5	GCA_009915155.1	2698827	2698827	type	True	77.4179	122	1072	95	below_threshold
Flavobacterium franklandianum	strain=LB3P56	GCA_007097365.1	2594430	2594430	type	True	77.3938	199	1072	95	below_threshold
Flavobacterium daejeonense	strain=DSM 17708	GCA_000425425.1	350893	350893	type	True	77.306	153	1072	95	below_threshold
Flavobacterium channae	strain=KSM-R2A30	GCA_021172165.1	2897181	2897181	type	True	77.2071	126	1072	95	below_threshold
Flavobacterium oncorhynchi	strain=CCUG 59446	GCA_002217355.1	728056	728056	type	True	77.2029	165	1072	95	below_threshold
Flavobacterium soyae	strain=SCIV07	GCA_021245985.1	2903098	2903098	type	True	77.089	161	1072	95	below_threshold
Flavobacterium sediminilitoris	strain=YSM-43	GCA_023008245.1	2024526	2024526	type	True	76.644	130	1072	95	below_threshold
Flavobacterium columnare	strain=ATCC 23463	GCA_002530675.1	996	996	type	True	76.4766	118	1072	95	below_threshold
Flavobacterium columnare	strain=NBRC 100251	GCA_007990835.1	996	996	type	True	76.4709	112	1072	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 11:52:32,738] [INFO] DFAST Taxonomy check result was written to GCF_002217205.1_ASM221720v1_genomic.fna/tc_result.tsv
[2024-01-24 11:52:32,739] [INFO] ===== Taxonomy check completed =====
[2024-01-24 11:52:32,739] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 11:52:32,740] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg9d074dd3-2052-4f82-bd35-0106c52de381/dqc_reference/checkm_data
[2024-01-24 11:52:32,741] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 11:52:32,779] [INFO] Task started: CheckM
[2024-01-24 11:52:32,780] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_002217205.1_ASM221720v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_002217205.1_ASM221720v1_genomic.fna/checkm_input GCF_002217205.1_ASM221720v1_genomic.fna/checkm_result
[2024-01-24 11:53:15,921] [INFO] Task succeeded: CheckM
[2024-01-24 11:53:15,923] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 11:53:15,946] [INFO] ===== Completeness check finished =====
[2024-01-24 11:53:15,946] [INFO] ===== Start GTDB Search =====
[2024-01-24 11:53:15,946] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_002217205.1_ASM221720v1_genomic.fna/markers.fasta)
[2024-01-24 11:53:15,947] [INFO] Task started: Blastn
[2024-01-24 11:53:15,947] [INFO] Running command: blastn -query GCF_002217205.1_ASM221720v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg9d074dd3-2052-4f82-bd35-0106c52de381/dqc_reference/reference_markers_gtdb.fasta -out GCF_002217205.1_ASM221720v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:53:16,781] [INFO] Task succeeded: Blastn
[2024-01-24 11:53:16,786] [INFO] Selected 29 target genomes.
[2024-01-24 11:53:16,786] [INFO] Target genome list was writen to GCF_002217205.1_ASM221720v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 11:53:16,813] [INFO] Task started: fastANI
[2024-01-24 11:53:16,813] [INFO] Running command: fastANI --query /var/lib/cwl/stg62de9ea0-09be-438e-b3cf-51d7d1096d47/GCF_002217205.1_ASM221720v1_genomic.fna.gz --refList GCF_002217205.1_ASM221720v1_genomic.fna/target_genomes_gtdb.txt --output GCF_002217205.1_ASM221720v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 11:53:32,161] [INFO] Task succeeded: fastANI
[2024-01-24 11:53:32,189] [INFO] Found 27 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 11:53:32,189] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_002217205.1	s__Flavobacterium branchiophilum	100.0	1060	1072	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Flavobacterium	95.0	98.60	97.23	0.92	0.84	5	conclusive
GCF_007109225.1	s__Flavobacterium sp007109225	77.8732	113	1072	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Flavobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002001005.1	s__Flavobacterium sp002001005	77.6968	159	1072	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Flavobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001831475.1	s__Flavobacterium crassostreae	77.557	148	1072	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Flavobacterium	95.0	99.99	99.99	1.00	1.00	2	-
GCF_015182285.1	s__Flavobacterium sp015182285	77.5461	150	1072	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Flavobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_009915155.1	s__Flavobacterium sp009915155	77.4378	121	1072	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Flavobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_007097265.1	s__Flavobacterium gawalongense	77.4363	171	1072	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Flavobacterium	95.0	99.69	99.40	0.97	0.94	5	-
GCF_008806775.1	s__Flavobacterium luteum	77.4121	188	1072	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Flavobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_018069925.1	s__Flavobacterium sp018069925	77.3922	152	1072	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Flavobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCA_903839195.1	s__Flavobacterium sp903839195	77.3652	145	1072	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Flavobacterium	95.0	98.63	96.46	0.85	0.82	4	-
GCA_004293165.1	s__Flavobacterium sp004293165	77.3533	131	1072	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Flavobacterium	95.0	97.30	97.30	0.81	0.81	2	-
GCF_000425425.1	s__Flavobacterium daejeonense	77.3059	153	1072	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Flavobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000708595.2	s__Flavobacterium sp000708595	77.298	167	1072	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Flavobacterium	95.0	98.30	98.27	0.95	0.93	3	-
GCF_009707955.1	s__Flavobacterium sp009707955	77.286	181	1072	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Flavobacterium	95.0	99.99	99.99	0.99	0.99	2	-
GCA_903879875.1	s__Flavobacterium sp903879875	77.2776	154	1072	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Flavobacterium	95.0	99.40	99.40	0.86	0.86	2	-
GCF_000812945.1	s__Flavobacterium sp000812945	77.1705	196	1072	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Flavobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_009495755.1	s__Flavobacterium sp009495755	77.1575	134	1072	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Flavobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCA_903819815.1	s__Flavobacterium sp903819815	77.0256	106	1072	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Flavobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCA_903837025.1	s__Flavobacterium sp903837025	77.016	106	1072	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Flavobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002217245.1	s__Flavobacterium columnare_A	76.9276	122	1072	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Flavobacterium	95.0	99.08	98.79	0.92	0.89	11	-
GCA_903824755.1	s__Flavobacterium sp903824755	76.8291	136	1072	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Flavobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002352205.1	s__Flavobacterium sp002352205	76.7974	147	1072	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Flavobacterium	95.0	99.95	99.95	0.86	0.86	2	-
GCA_003497625.1	s__Flavobacterium sp003497625	76.7867	125	1072	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Flavobacterium	95.0	99.98	99.98	0.98	0.98	2	-
GCF_004122145.1	s__Flavobacterium piscinae	76.7362	139	1072	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Flavobacterium	95.0	99.76	99.76	1.00	1.00	2	-
GCA_903888655.1	s__Flavobacterium sp903888655	76.6752	97	1072	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Flavobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002482975.1	s__Flavobacterium sp002482975	76.6461	174	1072	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Flavobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002530675.1	s__Flavobacterium columnare	76.4929	117	1072	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Flavobacteriales;f__Flavobacteriaceae;g__Flavobacterium	95.0	99.33	99.02	0.96	0.93	39	-
--------------------------------------------------------------------------------
[2024-01-24 11:53:32,191] [INFO] GTDB search result was written to GCF_002217205.1_ASM221720v1_genomic.fna/result_gtdb.tsv
[2024-01-24 11:53:32,191] [INFO] ===== GTDB Search completed =====
[2024-01-24 11:53:32,196] [INFO] DFAST_QC result json was written to GCF_002217205.1_ASM221720v1_genomic.fna/dqc_result.json
[2024-01-24 11:53:32,196] [INFO] DFAST_QC completed!
[2024-01-24 11:53:32,196] [INFO] Total running time: 0h1m28s