[2024-01-25 19:53:05,478] [INFO] DFAST_QC pipeline started.
[2024-01-25 19:53:05,480] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 19:53:05,481] [INFO] DQC Reference Directory: /var/lib/cwl/stgdda93eff-5eb7-409d-8415-7b6ca207d18a/dqc_reference
[2024-01-25 19:53:06,634] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 19:53:06,635] [INFO] Task started: Prodigal
[2024-01-25 19:53:06,635] [INFO] Running command: gunzip -c /var/lib/cwl/stge9274c5a-f2ac-4c9f-9cc9-526958d4a254/GCF_002494345.1_ASM249434v1_genomic.fna.gz | prodigal -d GCF_002494345.1_ASM249434v1_genomic.fna/cds.fna -a GCF_002494345.1_ASM249434v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 19:53:24,560] [INFO] Task succeeded: Prodigal
[2024-01-25 19:53:24,561] [INFO] Task started: HMMsearch
[2024-01-25 19:53:24,561] [INFO] Running command: hmmsearch --tblout GCF_002494345.1_ASM249434v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgdda93eff-5eb7-409d-8415-7b6ca207d18a/dqc_reference/reference_markers.hmm GCF_002494345.1_ASM249434v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 19:53:24,758] [INFO] Task succeeded: HMMsearch
[2024-01-25 19:53:24,759] [WARNING] Found 3/6 markers. [/var/lib/cwl/stge9274c5a-f2ac-4c9f-9cc9-526958d4a254/GCF_002494345.1_ASM249434v1_genomic.fna.gz]
[2024-01-25 19:53:24,794] [INFO] Query marker FASTA was written to GCF_002494345.1_ASM249434v1_genomic.fna/markers.fasta
[2024-01-25 19:53:24,794] [INFO] Task started: Blastn
[2024-01-25 19:53:24,794] [INFO] Running command: blastn -query GCF_002494345.1_ASM249434v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgdda93eff-5eb7-409d-8415-7b6ca207d18a/dqc_reference/reference_markers.fasta -out GCF_002494345.1_ASM249434v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 19:53:25,292] [INFO] Task succeeded: Blastn
[2024-01-25 19:53:25,294] [INFO] Selected 8 target genomes.
[2024-01-25 19:53:25,295] [INFO] Target genome list was writen to GCF_002494345.1_ASM249434v1_genomic.fna/target_genomes.txt
[2024-01-25 19:53:25,296] [INFO] Task started: fastANI
[2024-01-25 19:53:25,296] [INFO] Running command: fastANI --query /var/lib/cwl/stge9274c5a-f2ac-4c9f-9cc9-526958d4a254/GCF_002494345.1_ASM249434v1_genomic.fna.gz --refList GCF_002494345.1_ASM249434v1_genomic.fna/target_genomes.txt --output GCF_002494345.1_ASM249434v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 19:53:33,423] [INFO] Task succeeded: fastANI
[2024-01-25 19:53:33,424] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgdda93eff-5eb7-409d-8415-7b6ca207d18a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 19:53:33,424] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgdda93eff-5eb7-409d-8415-7b6ca207d18a/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 19:53:33,430] [INFO] Found 8 fastANI hits (0 hits with ANI > threshold)
[2024-01-25 19:53:33,430] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2024-01-25 19:53:33,430] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Natrinema amylolyticum	strain=LT61	GCA_020515625.1	2878679	2878679	type	True	85.5268	1003	1491	95	below_threshold
Natrinema altunense	strain=JCM 12890	GCA_000337155.1	222984	222984	type	True	85.3115	932	1491	95	below_threshold
Natrinema altunense	strain=AJ2	GCA_000731985.1	222984	222984	type	True	85.2932	959	1491	95	below_threshold
Natrinema salaciae	strain=DSM 25055	GCA_900110865.1	1186196	1186196	type	True	84.8758	1039	1491	95	below_threshold
Natrinema pallidum	strain=DSM 3751	GCA_000337615.1	69527	69527	type	True	84.8465	915	1491	95	below_threshold
Natrinema versiforme	strain=JCM 10478	GCA_000337195.1	88724	88724	type	True	84.4523	895	1491	95	below_threshold
Haloterrigena hispanica	strain=DSM 18328	GCA_004217335.1	392421	392421	type	True	82.4985	799	1491	95	below_threshold
Haloterrigena limicola	strain=JCM 13563	GCA_000337475.1	370323	370323	type	True	82.4534	756	1491	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 19:53:33,435] [INFO] DFAST Taxonomy check result was written to GCF_002494345.1_ASM249434v1_genomic.fna/tc_result.tsv
[2024-01-25 19:53:33,436] [INFO] ===== Taxonomy check completed =====
[2024-01-25 19:53:33,436] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 19:53:33,436] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgdda93eff-5eb7-409d-8415-7b6ca207d18a/dqc_reference/checkm_data
[2024-01-25 19:53:33,437] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 19:53:33,481] [INFO] Task started: CheckM
[2024-01-25 19:53:33,481] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_002494345.1_ASM249434v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_002494345.1_ASM249434v1_genomic.fna/checkm_input GCF_002494345.1_ASM249434v1_genomic.fna/checkm_result
[2024-01-25 19:54:21,845] [INFO] Task succeeded: CheckM
[2024-01-25 19:54:21,846] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 95.83%
Contamintation: 4.17%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 19:54:21,872] [INFO] ===== Completeness check finished =====
[2024-01-25 19:54:21,873] [INFO] ===== Start GTDB Search =====
[2024-01-25 19:54:21,874] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_002494345.1_ASM249434v1_genomic.fna/markers.fasta)
[2024-01-25 19:54:21,874] [INFO] Task started: Blastn
[2024-01-25 19:54:21,874] [INFO] Running command: blastn -query GCF_002494345.1_ASM249434v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgdda93eff-5eb7-409d-8415-7b6ca207d18a/dqc_reference/reference_markers_gtdb.fasta -out GCF_002494345.1_ASM249434v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 19:54:22,419] [INFO] Task succeeded: Blastn
[2024-01-25 19:54:22,421] [INFO] Selected 7 target genomes.
[2024-01-25 19:54:22,422] [INFO] Target genome list was writen to GCF_002494345.1_ASM249434v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 19:54:22,425] [INFO] Task started: fastANI
[2024-01-25 19:54:22,425] [INFO] Running command: fastANI --query /var/lib/cwl/stge9274c5a-f2ac-4c9f-9cc9-526958d4a254/GCF_002494345.1_ASM249434v1_genomic.fna.gz --refList GCF_002494345.1_ASM249434v1_genomic.fna/target_genomes_gtdb.txt --output GCF_002494345.1_ASM249434v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 19:54:30,395] [INFO] Task succeeded: fastANI
[2024-01-25 19:54:30,400] [INFO] Found 7 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 19:54:30,400] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_002494345.1	s__Natrinema ejinorense	100.0	1490	1491	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Natrialbaceae;g__Natrinema	95.0	95.42	95.42	0.91	0.91	2	conclusive
GCF_000731985.1	s__Natrinema altunense	85.3069	957	1491	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Natrialbaceae;g__Natrinema	95.0	98.98	98.00	0.96	0.93	4	-
GCF_900110865.1	s__Natrinema salaciae	84.8735	1040	1491	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Natrialbaceae;g__Natrinema	95.0	N/A	N/A	N/A	N/A	1	-
GCF_005576615.1	s__Natrinema versiforme_A	84.6716	947	1491	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Natrialbaceae;g__Natrinema	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000337195.1	s__Natrinema versiforme	84.468	894	1491	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Natrialbaceae;g__Natrinema	95.0	N/A	N/A	N/A	N/A	1	-
GCF_013456555.1	s__Natrinema sp013456555	83.9989	868	1491	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Natrialbaceae;g__Natrinema	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002572525.1	s__Natrinema sp002572525	83.922	939	1491	d__Archaea;p__Halobacteriota;c__Halobacteria;o__Halobacteriales;f__Natrialbaceae;g__Natrinema	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-25 19:54:30,401] [INFO] GTDB search result was written to GCF_002494345.1_ASM249434v1_genomic.fna/result_gtdb.tsv
[2024-01-25 19:54:30,403] [INFO] ===== GTDB Search completed =====
[2024-01-25 19:54:30,406] [INFO] DFAST_QC result json was written to GCF_002494345.1_ASM249434v1_genomic.fna/dqc_result.json
[2024-01-25 19:54:30,406] [INFO] DFAST_QC completed!
[2024-01-25 19:54:30,406] [INFO] Total running time: 0h1m25s