[2024-01-24 12:36:23,853] [INFO] DFAST_QC pipeline started.
[2024-01-24 12:36:23,856] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 12:36:23,856] [INFO] DQC Reference Directory: /var/lib/cwl/stg2cf2267a-318c-4026-af28-a2fedef53ef4/dqc_reference
[2024-01-24 12:36:25,185] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 12:36:25,186] [INFO] Task started: Prodigal
[2024-01-24 12:36:25,186] [INFO] Running command: gunzip -c /var/lib/cwl/stg2bdc2161-7a29-4fd6-9274-5ab057f64701/GCF_002864685.1_ASM286468v1_genomic.fna.gz | prodigal -d GCF_002864685.1_ASM286468v1_genomic.fna/cds.fna -a GCF_002864685.1_ASM286468v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 12:36:38,250] [INFO] Task succeeded: Prodigal
[2024-01-24 12:36:38,251] [INFO] Task started: HMMsearch
[2024-01-24 12:36:38,251] [INFO] Running command: hmmsearch --tblout GCF_002864685.1_ASM286468v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg2cf2267a-318c-4026-af28-a2fedef53ef4/dqc_reference/reference_markers.hmm GCF_002864685.1_ASM286468v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 12:36:38,517] [INFO] Task succeeded: HMMsearch
[2024-01-24 12:36:38,518] [INFO] Found 6/6 markers.
[2024-01-24 12:36:38,550] [INFO] Query marker FASTA was written to GCF_002864685.1_ASM286468v1_genomic.fna/markers.fasta
[2024-01-24 12:36:38,550] [INFO] Task started: Blastn
[2024-01-24 12:36:38,551] [INFO] Running command: blastn -query GCF_002864685.1_ASM286468v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg2cf2267a-318c-4026-af28-a2fedef53ef4/dqc_reference/reference_markers.fasta -out GCF_002864685.1_ASM286468v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:36:39,438] [INFO] Task succeeded: Blastn
[2024-01-24 12:36:39,442] [INFO] Selected 10 target genomes.
[2024-01-24 12:36:39,443] [INFO] Target genome list was writen to GCF_002864685.1_ASM286468v1_genomic.fna/target_genomes.txt
[2024-01-24 12:36:39,451] [INFO] Task started: fastANI
[2024-01-24 12:36:39,451] [INFO] Running command: fastANI --query /var/lib/cwl/stg2bdc2161-7a29-4fd6-9274-5ab057f64701/GCF_002864685.1_ASM286468v1_genomic.fna.gz --refList GCF_002864685.1_ASM286468v1_genomic.fna/target_genomes.txt --output GCF_002864685.1_ASM286468v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 12:36:48,686] [INFO] Task succeeded: fastANI
[2024-01-24 12:36:48,686] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg2cf2267a-318c-4026-af28-a2fedef53ef4/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 12:36:48,687] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg2cf2267a-318c-4026-af28-a2fedef53ef4/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 12:36:48,696] [INFO] Found 10 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 12:36:48,696] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 12:36:48,696] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Alcanivorax mobilis	strain=MT13131	GCA_002864685.1	2019569	2019569	type	True	100.0	1320	1324	95	conclusive
Alcanivorax venustensis	strain=ISO4	GCA_015356855.1	172371	172371	type	True	82.4657	797	1324	95	below_threshold
Alcanivorax marinus	strain=R8-12	GCA_025532125.1	1177169	1177169	type	True	82.429	793	1324	95	below_threshold
Alcanivorax profundimaris	strain=ST75FaO-1	GCA_015265435.1	2735259	2735259	type	True	82.3748	833	1324	95	below_threshold
Alcanivorax gelatiniphagus	strain=MEBiC 08158	GCA_005938655.1	1194167	1194167	type	True	82.2646	855	1324	95	below_threshold
Alcanivorax xenomutans	strain=JC109	GCA_900217905.1	1094342	1094342	type	True	79.6158	652	1324	95	below_threshold
Alcanivorax balearicus	strain=MACL04	GCA_025532145.1	413232	413232	type	True	79.595	657	1324	95	below_threshold
Alcanivorax limicola	strain=JB21	GCA_019931215.1	2874102	2874102	type	True	77.669	278	1324	95	below_threshold
Halomonas tianxiuensis	strain=BC-M4-5	GCA_009834345.1	2497861	2497861	type	True	76.8119	164	1324	95	below_threshold
Serratia entomophila	strain=A1	GCA_021462285.1	42906	42906	type	True	76.4132	104	1324	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 12:36:48,698] [INFO] DFAST Taxonomy check result was written to GCF_002864685.1_ASM286468v1_genomic.fna/tc_result.tsv
[2024-01-24 12:36:48,699] [INFO] ===== Taxonomy check completed =====
[2024-01-24 12:36:48,699] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 12:36:48,699] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg2cf2267a-318c-4026-af28-a2fedef53ef4/dqc_reference/checkm_data
[2024-01-24 12:36:48,702] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 12:36:48,750] [INFO] Task started: CheckM
[2024-01-24 12:36:48,751] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_002864685.1_ASM286468v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_002864685.1_ASM286468v1_genomic.fna/checkm_input GCF_002864685.1_ASM286468v1_genomic.fna/checkm_result
[2024-01-24 12:37:28,563] [INFO] Task succeeded: CheckM
[2024-01-24 12:37:28,564] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 12:37:28,583] [INFO] ===== Completeness check finished =====
[2024-01-24 12:37:28,584] [INFO] ===== Start GTDB Search =====
[2024-01-24 12:37:28,584] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_002864685.1_ASM286468v1_genomic.fna/markers.fasta)
[2024-01-24 12:37:28,585] [INFO] Task started: Blastn
[2024-01-24 12:37:28,585] [INFO] Running command: blastn -query GCF_002864685.1_ASM286468v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg2cf2267a-318c-4026-af28-a2fedef53ef4/dqc_reference/reference_markers_gtdb.fasta -out GCF_002864685.1_ASM286468v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:37:30,202] [INFO] Task succeeded: Blastn
[2024-01-24 12:37:30,206] [INFO] Selected 8 target genomes.
[2024-01-24 12:37:30,206] [INFO] Target genome list was writen to GCF_002864685.1_ASM286468v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 12:37:30,213] [INFO] Task started: fastANI
[2024-01-24 12:37:30,213] [INFO] Running command: fastANI --query /var/lib/cwl/stg2bdc2161-7a29-4fd6-9274-5ab057f64701/GCF_002864685.1_ASM286468v1_genomic.fna.gz --refList GCF_002864685.1_ASM286468v1_genomic.fna/target_genomes_gtdb.txt --output GCF_002864685.1_ASM286468v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 12:37:37,739] [INFO] Task succeeded: fastANI
[2024-01-24 12:37:37,753] [INFO] Found 8 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 12:37:37,753] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_002864685.1	s__Alcanivorax mobilis	100.0	1320	1324	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Alcanivoracaceae;g__Alcanivorax	95.0	98.57	98.57	0.96	0.96	2	conclusive
GCF_015356855.1	s__Alcanivorax venustensis	82.4754	796	1324	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Alcanivoracaceae;g__Alcanivorax	95.0	97.73	95.38	0.91	0.87	46	-
GCF_015265435.1	s__Alcanivorax profundimaris	82.3938	831	1324	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Alcanivoracaceae;g__Alcanivorax	95.0	97.49	97.23	0.85	0.77	11	-
GCF_016906305.1	s__Alcanivorax marinus_B	82.3768	858	1324	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Alcanivoracaceae;g__Alcanivorax	95.0	N/A	N/A	N/A	N/A	1	-
GCA_017794945.1	s__Alcanivorax sp009711585	82.2695	712	1324	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Alcanivoracaceae;g__Alcanivorax	95.0	96.99	96.99	0.93	0.93	2	-
GCF_005938655.1	s__Alcanivorax gelatiniphagus	82.2581	856	1324	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Alcanivoracaceae;g__Alcanivorax	95.0	98.53	98.53	0.93	0.93	3	-
GCA_002389055.1	s__Alcanivorax sp002389055	81.634	678	1324	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Alcanivoracaceae;g__Alcanivorax	95.0	96.98	96.27	0.78	0.74	3	-
GCF_016785095.1	s__Alcanivorax marinus_A	81.4917	808	1324	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Pseudomonadales;f__Alcanivoracaceae;g__Alcanivorax	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 12:37:37,755] [INFO] GTDB search result was written to GCF_002864685.1_ASM286468v1_genomic.fna/result_gtdb.tsv
[2024-01-24 12:37:37,756] [INFO] ===== GTDB Search completed =====
[2024-01-24 12:37:37,760] [INFO] DFAST_QC result json was written to GCF_002864685.1_ASM286468v1_genomic.fna/dqc_result.json
[2024-01-24 12:37:37,760] [INFO] DFAST_QC completed!
[2024-01-24 12:37:37,760] [INFO] Total running time: 0h1m14s