[2024-01-24 11:34:43,121] [INFO] DFAST_QC pipeline started.
[2024-01-24 11:34:43,122] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 11:34:43,123] [INFO] DQC Reference Directory: /var/lib/cwl/stg14f4ce96-58b3-4cf8-86c1-54ae0738aa87/dqc_reference
[2024-01-24 11:34:44,542] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 11:34:44,542] [INFO] Task started: Prodigal
[2024-01-24 11:34:44,543] [INFO] Running command: gunzip -c /var/lib/cwl/stga35d45e9-6b7f-4443-acb6-b6d7f1a79486/GCF_002915175.1_ASM291517v1_genomic.fna.gz | prodigal -d GCF_002915175.1_ASM291517v1_genomic.fna/cds.fna -a GCF_002915175.1_ASM291517v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 11:35:02,248] [INFO] Task succeeded: Prodigal
[2024-01-24 11:35:02,248] [INFO] Task started: HMMsearch
[2024-01-24 11:35:02,248] [INFO] Running command: hmmsearch --tblout GCF_002915175.1_ASM291517v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg14f4ce96-58b3-4cf8-86c1-54ae0738aa87/dqc_reference/reference_markers.hmm GCF_002915175.1_ASM291517v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 11:35:02,575] [INFO] Task succeeded: HMMsearch
[2024-01-24 11:35:02,576] [INFO] Found 6/6 markers.
[2024-01-24 11:35:02,629] [INFO] Query marker FASTA was written to GCF_002915175.1_ASM291517v1_genomic.fna/markers.fasta
[2024-01-24 11:35:02,630] [INFO] Task started: Blastn
[2024-01-24 11:35:02,630] [INFO] Running command: blastn -query GCF_002915175.1_ASM291517v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg14f4ce96-58b3-4cf8-86c1-54ae0738aa87/dqc_reference/reference_markers.fasta -out GCF_002915175.1_ASM291517v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:35:03,544] [INFO] Task succeeded: Blastn
[2024-01-24 11:35:03,546] [INFO] Selected 10 target genomes.
[2024-01-24 11:35:03,547] [INFO] Target genome list was writen to GCF_002915175.1_ASM291517v1_genomic.fna/target_genomes.txt
[2024-01-24 11:35:03,551] [INFO] Task started: fastANI
[2024-01-24 11:35:03,551] [INFO] Running command: fastANI --query /var/lib/cwl/stga35d45e9-6b7f-4443-acb6-b6d7f1a79486/GCF_002915175.1_ASM291517v1_genomic.fna.gz --refList GCF_002915175.1_ASM291517v1_genomic.fna/target_genomes.txt --output GCF_002915175.1_ASM291517v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 11:35:15,147] [INFO] Task succeeded: fastANI
[2024-01-24 11:35:15,147] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg14f4ce96-58b3-4cf8-86c1-54ae0738aa87/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 11:35:15,148] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg14f4ce96-58b3-4cf8-86c1-54ae0738aa87/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 11:35:15,157] [INFO] Found 10 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 11:35:15,157] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 11:35:15,157] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Agrobacterium rosae	strain=NCPPB 1650	GCA_002915175.1	1972867	1972867	type	True	100.0	1807	1810	95	conclusive
Agrobacterium bohemicum	strain=R90	GCA_002896715.1	2052828	2052828	type	True	86.7931	1211	1810	95	below_threshold
Agrobacterium rubi	strain=DSM 6772	GCA_017873055.1	28099	28099	type	True	85.7268	1186	1810	95	below_threshold
Agrobacterium rubi	strain=NBRC 13261	GCA_000739935.1	28099	28099	type	True	85.6301	1194	1810	95	below_threshold
Rhizobium skierniewicense	strain=Ch11	GCA_023757665.1	984260	984260	type	True	83.5833	1036	1810	95	below_threshold
Rhizobium skierniewicense	strain=DSM 26438	GCA_014196515.1	984260	984260	type	True	83.5504	1047	1810	95	below_threshold
Agrobacterium leguminum	strain=MOPV5	GCA_015704895.1	2792015	2792015	type	True	80.3845	808	1810	95	below_threshold
Neorhizobium lilium	strain=24NR	GCA_004053875.1	2503024	2503024	type	True	78.1017	450	1810	95	below_threshold
Rhizobium petrolearium	strain=DSM 26482	GCA_017873175.1	515361	515361	type	True	77.8914	433	1810	95	below_threshold
Ciceribacter ferrooxidans	strain=F8825	GCA_004137355.1	2509717	2509717	type	True	77.5124	365	1810	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 11:35:15,159] [INFO] DFAST Taxonomy check result was written to GCF_002915175.1_ASM291517v1_genomic.fna/tc_result.tsv
[2024-01-24 11:35:15,159] [INFO] ===== Taxonomy check completed =====
[2024-01-24 11:35:15,159] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 11:35:15,160] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg14f4ce96-58b3-4cf8-86c1-54ae0738aa87/dqc_reference/checkm_data
[2024-01-24 11:35:15,161] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 11:35:15,212] [INFO] Task started: CheckM
[2024-01-24 11:35:15,212] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_002915175.1_ASM291517v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_002915175.1_ASM291517v1_genomic.fna/checkm_input GCF_002915175.1_ASM291517v1_genomic.fna/checkm_result
[2024-01-24 11:36:06,567] [INFO] Task succeeded: CheckM
[2024-01-24 11:36:06,568] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 11:36:06,587] [INFO] ===== Completeness check finished =====
[2024-01-24 11:36:06,588] [INFO] ===== Start GTDB Search =====
[2024-01-24 11:36:06,588] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_002915175.1_ASM291517v1_genomic.fna/markers.fasta)
[2024-01-24 11:36:06,589] [INFO] Task started: Blastn
[2024-01-24 11:36:06,589] [INFO] Running command: blastn -query GCF_002915175.1_ASM291517v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg14f4ce96-58b3-4cf8-86c1-54ae0738aa87/dqc_reference/reference_markers_gtdb.fasta -out GCF_002915175.1_ASM291517v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:36:08,218] [INFO] Task succeeded: Blastn
[2024-01-24 11:36:08,221] [INFO] Selected 8 target genomes.
[2024-01-24 11:36:08,222] [INFO] Target genome list was writen to GCF_002915175.1_ASM291517v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 11:36:08,229] [INFO] Task started: fastANI
[2024-01-24 11:36:08,229] [INFO] Running command: fastANI --query /var/lib/cwl/stga35d45e9-6b7f-4443-acb6-b6d7f1a79486/GCF_002915175.1_ASM291517v1_genomic.fna.gz --refList GCF_002915175.1_ASM291517v1_genomic.fna/target_genomes_gtdb.txt --output GCF_002915175.1_ASM291517v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 11:36:18,723] [INFO] Task succeeded: fastANI
[2024-01-24 11:36:18,731] [INFO] Found 8 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 11:36:18,731] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_002915175.1	s__Agrobacterium rosae	100.0	1807	1810	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Rhizobiaceae;g__Agrobacterium	95.0	97.78	95.28	0.91	0.82	8	conclusive
GCF_002896715.1	s__Agrobacterium bohemicum	86.8098	1209	1810	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Rhizobiaceae;g__Agrobacterium	95.0	99.81	99.81	1.00	1.00	2	-
GCA_900472925.1	s__Agrobacterium sp900472925	86.1294	1190	1810	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Rhizobiaceae;g__Agrobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCA_900473205.1	s__Agrobacterium sp900473205	85.7967	1200	1810	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Rhizobiaceae;g__Agrobacterium	95.0	99.99	99.99	0.99	0.99	2	-
GCF_000739935.1	s__Agrobacterium rubi	85.6296	1195	1810	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Rhizobiaceae;g__Agrobacterium	95.0	96.45	95.90	0.89	0.87	10	-
GCF_001422245.1	s__Agrobacterium sp001422245	84.7685	1066	1810	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Rhizobiaceae;g__Agrobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCA_900468015.1	s__Agrobacterium sp900468015	84.5645	1113	1810	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Rhizobiaceae;g__Agrobacterium	95.0	95.47	95.47	0.86	0.86	2	-
GCF_014196515.1	s__Agrobacterium skierniewicense	83.5654	1045	1810	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhizobiales;f__Rhizobiaceae;g__Agrobacterium	95.0	98.07	98.07	0.91	0.91	2	-
--------------------------------------------------------------------------------
[2024-01-24 11:36:18,733] [INFO] GTDB search result was written to GCF_002915175.1_ASM291517v1_genomic.fna/result_gtdb.tsv
[2024-01-24 11:36:18,733] [INFO] ===== GTDB Search completed =====
[2024-01-24 11:36:18,738] [INFO] DFAST_QC result json was written to GCF_002915175.1_ASM291517v1_genomic.fna/dqc_result.json
[2024-01-24 11:36:18,739] [INFO] DFAST_QC completed!
[2024-01-24 11:36:18,739] [INFO] Total running time: 0h1m36s