[2024-01-24 13:41:20,625] [INFO] DFAST_QC pipeline started.
[2024-01-24 13:41:20,627] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 13:41:20,627] [INFO] DQC Reference Directory: /var/lib/cwl/stg579ef7de-5ec1-4737-a7ad-4f5db6c971f1/dqc_reference
[2024-01-24 13:41:21,922] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 13:41:21,923] [INFO] Task started: Prodigal
[2024-01-24 13:41:21,923] [INFO] Running command: gunzip -c /var/lib/cwl/stg784750b6-b98a-4ce0-ba83-3496a0738b77/GCF_002925905.1_ASM292590v1_genomic.fna.gz | prodigal -d GCF_002925905.1_ASM292590v1_genomic.fna/cds.fna -a GCF_002925905.1_ASM292590v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 13:41:38,321] [INFO] Task succeeded: Prodigal
[2024-01-24 13:41:38,322] [INFO] Task started: HMMsearch
[2024-01-24 13:41:38,322] [INFO] Running command: hmmsearch --tblout GCF_002925905.1_ASM292590v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg579ef7de-5ec1-4737-a7ad-4f5db6c971f1/dqc_reference/reference_markers.hmm GCF_002925905.1_ASM292590v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 13:41:38,719] [INFO] Task succeeded: HMMsearch
[2024-01-24 13:41:38,720] [INFO] Found 6/6 markers.
[2024-01-24 13:41:38,771] [INFO] Query marker FASTA was written to GCF_002925905.1_ASM292590v1_genomic.fna/markers.fasta
[2024-01-24 13:41:38,772] [INFO] Task started: Blastn
[2024-01-24 13:41:38,772] [INFO] Running command: blastn -query GCF_002925905.1_ASM292590v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg579ef7de-5ec1-4737-a7ad-4f5db6c971f1/dqc_reference/reference_markers.fasta -out GCF_002925905.1_ASM292590v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:41:39,755] [INFO] Task succeeded: Blastn
[2024-01-24 13:41:39,758] [INFO] Selected 12 target genomes.
[2024-01-24 13:41:39,759] [INFO] Target genome list was writen to GCF_002925905.1_ASM292590v1_genomic.fna/target_genomes.txt
[2024-01-24 13:41:39,763] [INFO] Task started: fastANI
[2024-01-24 13:41:39,764] [INFO] Running command: fastANI --query /var/lib/cwl/stg784750b6-b98a-4ce0-ba83-3496a0738b77/GCF_002925905.1_ASM292590v1_genomic.fna.gz --refList GCF_002925905.1_ASM292590v1_genomic.fna/target_genomes.txt --output GCF_002925905.1_ASM292590v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 13:41:57,185] [INFO] Task succeeded: fastANI
[2024-01-24 13:41:57,186] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg579ef7de-5ec1-4737-a7ad-4f5db6c971f1/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 13:41:57,186] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg579ef7de-5ec1-4737-a7ad-4f5db6c971f1/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 13:41:57,196] [INFO] Found 12 fastANI hits (2 hits with ANI > threshold)
[2024-01-24 13:41:57,197] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 13:41:57,197] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Klebsiella michiganensis	strain=DSM 25444	GCA_002925905.1	1134687	1134687	type	True	100.0	2045	2047	95	conclusive
Klebsiella michiganensis	strain=CCUG 66515	GCA_009173485.1	1134687	1134687	type	True	99.9714	1965	2047	95	conclusive
Klebsiella pasteurii		GCA_902158725.1	2587529	2587529	type	True	93.9922	1731	2047	95	below_threshold
Klebsiella grimontii	strain=06D021	GCA_900200035.1	2058152	2058152	type	True	93.8177	1703	2047	95	below_threshold
Klebsiella oxytoca	strain=DSM 5175	GCA_020115535.1	571	571	type	True	92.4997	1607	2047	95	below_threshold
Klebsiella oxytoca	strain=NCTC13727	GCA_900636985.1	571	571	type	True	92.4915	1649	2047	95	below_threshold
Klebsiella oxytoca	strain=NBRC 105695	GCA_001598695.1	571	571	type	True	92.4437	1625	2047	95	below_threshold
Klebsiella pneumoniae	strain=PartO-Kpneumoniae-RM8376	GCA_022869665.1	573	573	type	True	84.5863	1311	2047	95	below_threshold
Enterobacter roggenkampii	strain=DSM 16690	GCA_024390995.1	1812935	1812935	type	True	82.216	972	2047	95	below_threshold
Citrobacter rodentium	strain=DSM 16636	GCA_021278985.1	67825	67825	type	True	82.0348	961	2047	95	below_threshold
Enterobacter wuhouensis	strain=WCHEW120002	GCA_004331265.1	2529381	2529381	type	True	81.8822	975	2047	95	below_threshold
Enterobacter sichuanensis	strain=WCHECL1597	GCA_025002605.1	2071710	2071710	type	True	81.5368	980	2047	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 13:41:57,199] [INFO] DFAST Taxonomy check result was written to GCF_002925905.1_ASM292590v1_genomic.fna/tc_result.tsv
[2024-01-24 13:41:57,199] [INFO] ===== Taxonomy check completed =====
[2024-01-24 13:41:57,199] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 13:41:57,200] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg579ef7de-5ec1-4737-a7ad-4f5db6c971f1/dqc_reference/checkm_data
[2024-01-24 13:41:57,201] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 13:41:57,263] [INFO] Task started: CheckM
[2024-01-24 13:41:57,263] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_002925905.1_ASM292590v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_002925905.1_ASM292590v1_genomic.fna/checkm_input GCF_002925905.1_ASM292590v1_genomic.fna/checkm_result
[2024-01-24 13:42:44,614] [INFO] Task succeeded: CheckM
[2024-01-24 13:42:44,616] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 98.96%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 13:42:44,637] [INFO] ===== Completeness check finished =====
[2024-01-24 13:42:44,638] [INFO] ===== Start GTDB Search =====
[2024-01-24 13:42:44,638] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_002925905.1_ASM292590v1_genomic.fna/markers.fasta)
[2024-01-24 13:42:44,639] [INFO] Task started: Blastn
[2024-01-24 13:42:44,639] [INFO] Running command: blastn -query GCF_002925905.1_ASM292590v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg579ef7de-5ec1-4737-a7ad-4f5db6c971f1/dqc_reference/reference_markers_gtdb.fasta -out GCF_002925905.1_ASM292590v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:42:46,153] [INFO] Task succeeded: Blastn
[2024-01-24 13:42:46,157] [INFO] Selected 7 target genomes.
[2024-01-24 13:42:46,158] [INFO] Target genome list was writen to GCF_002925905.1_ASM292590v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 13:42:46,165] [INFO] Task started: fastANI
[2024-01-24 13:42:46,166] [INFO] Running command: fastANI --query /var/lib/cwl/stg784750b6-b98a-4ce0-ba83-3496a0738b77/GCF_002925905.1_ASM292590v1_genomic.fna.gz --refList GCF_002925905.1_ASM292590v1_genomic.fna/target_genomes_gtdb.txt --output GCF_002925905.1_ASM292590v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 13:42:57,361] [INFO] Task succeeded: fastANI
[2024-01-24 13:42:57,369] [INFO] Found 7 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 13:42:57,369] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_002925905.1	s__Klebsiella michiganensis	100.0	2044	2047	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Klebsiella	95.0	98.53	97.41	0.90	0.83	291	conclusive
GCF_900200035.1	s__Klebsiella grimontii	93.8174	1704	2047	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Klebsiella	95.0	98.56	95.68	0.89	0.83	175	-
GCF_001598695.1	s__Klebsiella oxytoca	92.4383	1626	2047	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Klebsiella	95.0	99.31	98.97	0.94	0.89	181	-
GCF_009707385.1	s__Klebsiella oxytoca_C	90.2648	1525	2047	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Klebsiella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_902158555.1	s__Klebsiella spallanzanii	89.3804	1521	2047	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Klebsiella	95.0	98.75	98.01	0.91	0.88	4	-
GCF_003261575.2	s__Klebsiella huaxiensis	88.323	1550	2047	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Klebsiella	95.0	96.95	95.21	0.89	0.84	9	-
GCF_005860775.1	s__Klebsiella indica	86.4011	1292	2047	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Enterobacterales;f__Enterobacteriaceae;g__Klebsiella	95.0	99.34	99.34	0.92	0.92	2	-
--------------------------------------------------------------------------------
[2024-01-24 13:42:57,371] [INFO] GTDB search result was written to GCF_002925905.1_ASM292590v1_genomic.fna/result_gtdb.tsv
[2024-01-24 13:42:57,372] [INFO] ===== GTDB Search completed =====
[2024-01-24 13:42:57,376] [INFO] DFAST_QC result json was written to GCF_002925905.1_ASM292590v1_genomic.fna/dqc_result.json
[2024-01-24 13:42:57,376] [INFO] DFAST_QC completed!
[2024-01-24 13:42:57,376] [INFO] Total running time: 0h1m37s