[2024-01-25 18:05:50,642] [INFO] DFAST_QC pipeline started.
[2024-01-25 18:05:50,643] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 18:05:50,643] [INFO] DQC Reference Directory: /var/lib/cwl/stg5ef44f24-4856-4808-9eaf-6bf3dc85fa6f/dqc_reference
[2024-01-25 18:05:51,765] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 18:05:51,766] [INFO] Task started: Prodigal
[2024-01-25 18:05:51,766] [INFO] Running command: gunzip -c /var/lib/cwl/stg9ad012e8-7e39-4d43-b839-df2688c25002/GCF_003172895.1_ASM317289v1_genomic.fna.gz | prodigal -d GCF_003172895.1_ASM317289v1_genomic.fna/cds.fna -a GCF_003172895.1_ASM317289v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 18:06:00,858] [INFO] Task succeeded: Prodigal
[2024-01-25 18:06:00,859] [INFO] Task started: HMMsearch
[2024-01-25 18:06:00,859] [INFO] Running command: hmmsearch --tblout GCF_003172895.1_ASM317289v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg5ef44f24-4856-4808-9eaf-6bf3dc85fa6f/dqc_reference/reference_markers.hmm GCF_003172895.1_ASM317289v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 18:06:01,111] [INFO] Task succeeded: HMMsearch
[2024-01-25 18:06:01,112] [INFO] Found 6/6 markers.
[2024-01-25 18:06:01,151] [INFO] Query marker FASTA was written to GCF_003172895.1_ASM317289v1_genomic.fna/markers.fasta
[2024-01-25 18:06:01,151] [INFO] Task started: Blastn
[2024-01-25 18:06:01,151] [INFO] Running command: blastn -query GCF_003172895.1_ASM317289v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg5ef44f24-4856-4808-9eaf-6bf3dc85fa6f/dqc_reference/reference_markers.fasta -out GCF_003172895.1_ASM317289v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 18:06:01,742] [INFO] Task succeeded: Blastn
[2024-01-25 18:06:01,744] [INFO] Selected 22 target genomes.
[2024-01-25 18:06:01,745] [INFO] Target genome list was writen to GCF_003172895.1_ASM317289v1_genomic.fna/target_genomes.txt
[2024-01-25 18:06:01,759] [INFO] Task started: fastANI
[2024-01-25 18:06:01,759] [INFO] Running command: fastANI --query /var/lib/cwl/stg9ad012e8-7e39-4d43-b839-df2688c25002/GCF_003172895.1_ASM317289v1_genomic.fna.gz --refList GCF_003172895.1_ASM317289v1_genomic.fna/target_genomes.txt --output GCF_003172895.1_ASM317289v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 18:06:16,548] [INFO] Task succeeded: fastANI
[2024-01-25 18:06:16,548] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg5ef44f24-4856-4808-9eaf-6bf3dc85fa6f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 18:06:16,549] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg5ef44f24-4856-4808-9eaf-6bf3dc85fa6f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 18:06:16,553] [INFO] Found 4 fastANI hits (1 hits with ANI > threshold)
[2024-01-25 18:06:16,553] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-25 18:06:16,554] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Leucothrix arctica	strain=IMCC9719	GCA_003172895.1	1481894	1481894	type	True	100.0	1558	1561	95	conclusive
Leucothrix pacifica	strain=JCM 18388	GCA_003172875.1	1247513	1247513	type	True	78.3283	249	1561	95	below_threshold
Leucothrix mucor	strain=DSM 2157	GCA_000419525.1	45248	45248	type	True	77.9827	228	1561	95	below_threshold
Cocleimonas flava	strain=DSM 24830	GCA_004339015.1	634765	634765	type	True	76.3715	65	1561	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 18:06:16,555] [INFO] DFAST Taxonomy check result was written to GCF_003172895.1_ASM317289v1_genomic.fna/tc_result.tsv
[2024-01-25 18:06:16,555] [INFO] ===== Taxonomy check completed =====
[2024-01-25 18:06:16,555] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 18:06:16,556] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg5ef44f24-4856-4808-9eaf-6bf3dc85fa6f/dqc_reference/checkm_data
[2024-01-25 18:06:16,556] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 18:06:16,609] [INFO] Task started: CheckM
[2024-01-25 18:06:16,609] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_003172895.1_ASM317289v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_003172895.1_ASM317289v1_genomic.fna/checkm_input GCF_003172895.1_ASM317289v1_genomic.fna/checkm_result
[2024-01-25 18:06:47,029] [INFO] Task succeeded: CheckM
[2024-01-25 18:06:47,032] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 18:06:47,050] [INFO] ===== Completeness check finished =====
[2024-01-25 18:06:47,050] [INFO] ===== Start GTDB Search =====
[2024-01-25 18:06:47,051] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_003172895.1_ASM317289v1_genomic.fna/markers.fasta)
[2024-01-25 18:06:47,051] [INFO] Task started: Blastn
[2024-01-25 18:06:47,051] [INFO] Running command: blastn -query GCF_003172895.1_ASM317289v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg5ef44f24-4856-4808-9eaf-6bf3dc85fa6f/dqc_reference/reference_markers_gtdb.fasta -out GCF_003172895.1_ASM317289v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 18:06:47,856] [INFO] Task succeeded: Blastn
[2024-01-25 18:06:47,858] [INFO] Selected 19 target genomes.
[2024-01-25 18:06:47,858] [INFO] Target genome list was writen to GCF_003172895.1_ASM317289v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 18:06:47,885] [INFO] Task started: fastANI
[2024-01-25 18:06:47,885] [INFO] Running command: fastANI --query /var/lib/cwl/stg9ad012e8-7e39-4d43-b839-df2688c25002/GCF_003172895.1_ASM317289v1_genomic.fna.gz --refList GCF_003172895.1_ASM317289v1_genomic.fna/target_genomes_gtdb.txt --output GCF_003172895.1_ASM317289v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 18:06:59,840] [INFO] Task succeeded: fastANI
[2024-01-25 18:06:59,845] [INFO] Found 6 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 18:06:59,845] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_003172895.1	s__Leucothrix arctica	100.0	1558	1561	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Thiotrichales;f__Thiotrichaceae;g__Leucothrix	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCF_003172875.1	s__Leucothrix pacifica	78.3423	248	1561	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Thiotrichales;f__Thiotrichaceae;g__Leucothrix	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000419525.1	s__Leucothrix mucor	77.9687	227	1561	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Thiotrichales;f__Thiotrichaceae;g__Leucothrix	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002747455.1	s__Leucothrix sp002747455	76.9798	94	1561	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Thiotrichales;f__Thiotrichaceae;g__Leucothrix	95.0	N/A	N/A	N/A	N/A	1	-
GCF_004339015.1	s__Cocleimonas flava	76.3715	65	1561	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Thiotrichales;f__Thiotrichaceae;g__Cocleimonas	95.0	N/A	N/A	N/A	N/A	1	-
GCA_902727985.1	s__HyVt-477 sp902727985	75.9365	50	1561	d__Bacteria;p__Proteobacteria;c__Gammaproteobacteria;o__Thiotrichales;f__Thiotrichaceae;g__HyVt-477	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-25 18:06:59,846] [INFO] GTDB search result was written to GCF_003172895.1_ASM317289v1_genomic.fna/result_gtdb.tsv
[2024-01-25 18:06:59,847] [INFO] ===== GTDB Search completed =====
[2024-01-25 18:06:59,850] [INFO] DFAST_QC result json was written to GCF_003172895.1_ASM317289v1_genomic.fna/dqc_result.json
[2024-01-25 18:06:59,850] [INFO] DFAST_QC completed!
[2024-01-25 18:06:59,850] [INFO] Total running time: 0h1m9s