[2024-01-24 12:54:21,406] [INFO] DFAST_QC pipeline started.
[2024-01-24 12:54:21,408] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 12:54:21,408] [INFO] DQC Reference Directory: /var/lib/cwl/stg41f70df1-1c9e-48f5-a91c-acf999c2c385/dqc_reference
[2024-01-24 12:54:23,877] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 12:54:23,878] [INFO] Task started: Prodigal
[2024-01-24 12:54:23,879] [INFO] Running command: gunzip -c /var/lib/cwl/stg28fbee7e-6f3a-46ed-b93f-f32a1fa897ff/GCF_003245365.1_ASM324536v1_genomic.fna.gz | prodigal -d GCF_003245365.1_ASM324536v1_genomic.fna/cds.fna -a GCF_003245365.1_ASM324536v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 12:54:27,632] [INFO] Task succeeded: Prodigal
[2024-01-24 12:54:27,632] [INFO] Task started: HMMsearch
[2024-01-24 12:54:27,632] [INFO] Running command: hmmsearch --tblout GCF_003245365.1_ASM324536v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg41f70df1-1c9e-48f5-a91c-acf999c2c385/dqc_reference/reference_markers.hmm GCF_003245365.1_ASM324536v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 12:54:27,878] [INFO] Task succeeded: HMMsearch
[2024-01-24 12:54:27,880] [INFO] Found 6/6 markers.
[2024-01-24 12:54:27,905] [INFO] Query marker FASTA was written to GCF_003245365.1_ASM324536v1_genomic.fna/markers.fasta
[2024-01-24 12:54:27,905] [INFO] Task started: Blastn
[2024-01-24 12:54:27,905] [INFO] Running command: blastn -query GCF_003245365.1_ASM324536v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg41f70df1-1c9e-48f5-a91c-acf999c2c385/dqc_reference/reference_markers.fasta -out GCF_003245365.1_ASM324536v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:54:28,756] [INFO] Task succeeded: Blastn
[2024-01-24 12:54:28,760] [INFO] Selected 8 target genomes.
[2024-01-24 12:54:28,761] [INFO] Target genome list was writen to GCF_003245365.1_ASM324536v1_genomic.fna/target_genomes.txt
[2024-01-24 12:54:28,793] [INFO] Task started: fastANI
[2024-01-24 12:54:28,794] [INFO] Running command: fastANI --query /var/lib/cwl/stg28fbee7e-6f3a-46ed-b93f-f32a1fa897ff/GCF_003245365.1_ASM324536v1_genomic.fna.gz --refList GCF_003245365.1_ASM324536v1_genomic.fna/target_genomes.txt --output GCF_003245365.1_ASM324536v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 12:54:31,935] [INFO] Task succeeded: fastANI
[2024-01-24 12:54:31,935] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg41f70df1-1c9e-48f5-a91c-acf999c2c385/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 12:54:31,936] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg41f70df1-1c9e-48f5-a91c-acf999c2c385/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 12:54:31,988] [INFO] Found 8 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 12:54:31,988] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 12:54:31,989] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Helicobacter valdiviensis	strain=WBE14	GCA_003245365.1	1458358	1458358	type	True	100.0	668	671	95	conclusive
Helicobacter burdigaliensis	strain=CNRCH 2005/566H	GCA_003670295.1	2315334	2315334	type	True	85.3826	502	671	95	below_threshold
Helicobacter pullorum	strain=CCUG 33837	GCA_017979475.1	35818	35818	type	True	78.275	191	671	95	below_threshold
Helicobacter winghamensis	strain=ATCC BAA-430	GCA_000158455.1	157268	157268	type	True	78.1954	184	671	95	below_threshold
Helicobacter canadensis	strain=MIT 98-5491	GCA_000155455.1	123841	123841	type	True	77.8307	199	671	95	below_threshold
Helicobacter canadensis	strain=MIT 98-5491	GCA_000162575.1	123841	123841	type	True	77.8215	204	671	95	below_threshold
Helicobacter turcicus	strain=Faydin-H70	GCA_019711685.1	2867412	2867412	type	True	77.6818	165	671	95	below_threshold
Helicobacter rodentium	strain=ATCC 700285	GCA_000687535.1	59617	59617	type	True	77.5334	144	671	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 12:54:31,991] [INFO] DFAST Taxonomy check result was written to GCF_003245365.1_ASM324536v1_genomic.fna/tc_result.tsv
[2024-01-24 12:54:31,992] [INFO] ===== Taxonomy check completed =====
[2024-01-24 12:54:31,992] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 12:54:31,992] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg41f70df1-1c9e-48f5-a91c-acf999c2c385/dqc_reference/checkm_data
[2024-01-24 12:54:31,995] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 12:54:32,023] [INFO] Task started: CheckM
[2024-01-24 12:54:32,024] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_003245365.1_ASM324536v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_003245365.1_ASM324536v1_genomic.fna/checkm_input GCF_003245365.1_ASM324536v1_genomic.fna/checkm_result
[2024-01-24 12:54:52,993] [INFO] Task succeeded: CheckM
[2024-01-24 12:54:52,995] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 12:54:53,017] [INFO] ===== Completeness check finished =====
[2024-01-24 12:54:53,018] [INFO] ===== Start GTDB Search =====
[2024-01-24 12:54:53,018] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_003245365.1_ASM324536v1_genomic.fna/markers.fasta)
[2024-01-24 12:54:53,019] [INFO] Task started: Blastn
[2024-01-24 12:54:53,019] [INFO] Running command: blastn -query GCF_003245365.1_ASM324536v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg41f70df1-1c9e-48f5-a91c-acf999c2c385/dqc_reference/reference_markers_gtdb.fasta -out GCF_003245365.1_ASM324536v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:54:53,794] [INFO] Task succeeded: Blastn
[2024-01-24 12:54:53,798] [INFO] Selected 11 target genomes.
[2024-01-24 12:54:53,798] [INFO] Target genome list was writen to GCF_003245365.1_ASM324536v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 12:54:53,806] [INFO] Task started: fastANI
[2024-01-24 12:54:53,806] [INFO] Running command: fastANI --query /var/lib/cwl/stg28fbee7e-6f3a-46ed-b93f-f32a1fa897ff/GCF_003245365.1_ASM324536v1_genomic.fna.gz --refList GCF_003245365.1_ASM324536v1_genomic.fna/target_genomes_gtdb.txt --output GCF_003245365.1_ASM324536v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 12:54:57,882] [INFO] Task succeeded: fastANI
[2024-01-24 12:54:57,898] [INFO] Found 11 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 12:54:57,898] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_003245365.1	s__Helicobacter_D valdiviensis	100.0	668	671	d__Bacteria;p__Campylobacterota;c__Campylobacteria;o__Campylobacterales;f__Helicobacteraceae;g__Helicobacter_D	95.0	N/A	N/A	N/A	N/A	1	conclusive
GCF_003670295.1	s__Helicobacter_D burdigaliensis	85.3723	503	671	d__Bacteria;p__Campylobacterota;c__Campylobacteria;o__Campylobacterales;f__Helicobacteraceae;g__Helicobacter_D	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000765695.2	s__Helicobacter_D sp000765695	78.2611	186	671	d__Bacteria;p__Campylobacterota;c__Campylobacteria;o__Campylobacterales;f__Helicobacteraceae;g__Helicobacter_D	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000158455.1	s__Helicobacter_D winghamensis	78.2155	183	671	d__Bacteria;p__Campylobacterota;c__Campylobacteria;o__Campylobacterales;f__Helicobacteraceae;g__Helicobacter_D	95.0	99.73	99.12	0.98	0.96	8	-
GCF_008801855.1	s__Helicobacter_D pullorum	78.1665	202	671	d__Bacteria;p__Campylobacterota;c__Campylobacteria;o__Campylobacterales;f__Helicobacteraceae;g__Helicobacter_D	95.0	97.98	97.63	0.93	0.87	29	-
GCF_003288905.1	s__Helicobacter_D sp003288905	78.1362	193	671	d__Bacteria;p__Campylobacterota;c__Campylobacteria;o__Campylobacterales;f__Helicobacteraceae;g__Helicobacter_D	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000162575.1	s__Helicobacter_D canadensis	77.8228	204	671	d__Bacteria;p__Campylobacterota;c__Campylobacteria;o__Campylobacterales;f__Helicobacteraceae;g__Helicobacter_D	95.0	99.98	99.92	1.00	0.99	5	-
GCF_900198605.1	s__Helicobacter_D mesocricetorum	77.6967	164	671	d__Bacteria;p__Campylobacterota;c__Campylobacteria;o__Campylobacterales;f__Helicobacteraceae;g__Helicobacter_D	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000765745.2	s__Helicobacter_D apodemus	77.5782	172	671	d__Bacteria;p__Campylobacterota;c__Campylobacteria;o__Campylobacterales;f__Helicobacteraceae;g__Helicobacter_D	95.0	98.27	97.09	0.93	0.91	3	-
GCF_000765675.1	s__Helicobacter_D sp000765675	77.5204	136	671	d__Bacteria;p__Campylobacterota;c__Campylobacteria;o__Campylobacterales;f__Helicobacteraceae;g__Helicobacter_D	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003364315.1	s__Helicobacter_D ganmani	77.4422	138	671	d__Bacteria;p__Campylobacterota;c__Campylobacteria;o__Campylobacterales;f__Helicobacteraceae;g__Helicobacter_D	95.0	99.45	99.17	0.96	0.93	4	-
--------------------------------------------------------------------------------
[2024-01-24 12:54:57,900] [INFO] GTDB search result was written to GCF_003245365.1_ASM324536v1_genomic.fna/result_gtdb.tsv
[2024-01-24 12:54:57,901] [INFO] ===== GTDB Search completed =====
[2024-01-24 12:54:57,905] [INFO] DFAST_QC result json was written to GCF_003245365.1_ASM324536v1_genomic.fna/dqc_result.json
[2024-01-24 12:54:57,905] [INFO] DFAST_QC completed!
[2024-01-24 12:54:57,905] [INFO] Total running time: 0h0m36s