[2024-01-24 14:06:29,294] [INFO] DFAST_QC pipeline started.
[2024-01-24 14:06:29,295] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 14:06:29,296] [INFO] DQC Reference Directory: /var/lib/cwl/stga0a41cb8-7643-4c47-836a-29c376be3294/dqc_reference
[2024-01-24 14:06:30,587] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 14:06:30,588] [INFO] Task started: Prodigal
[2024-01-24 14:06:30,589] [INFO] Running command: gunzip -c /var/lib/cwl/stg04415fe0-1852-4926-a228-23f4913521e6/GCF_003261095.1_ASM326109v1_genomic.fna.gz | prodigal -d GCF_003261095.1_ASM326109v1_genomic.fna/cds.fna -a GCF_003261095.1_ASM326109v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 14:06:44,527] [INFO] Task succeeded: Prodigal
[2024-01-24 14:06:44,527] [INFO] Task started: HMMsearch
[2024-01-24 14:06:44,528] [INFO] Running command: hmmsearch --tblout GCF_003261095.1_ASM326109v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stga0a41cb8-7643-4c47-836a-29c376be3294/dqc_reference/reference_markers.hmm GCF_003261095.1_ASM326109v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 14:06:44,881] [INFO] Task succeeded: HMMsearch
[2024-01-24 14:06:44,883] [INFO] Found 6/6 markers.
[2024-01-24 14:06:44,940] [INFO] Query marker FASTA was written to GCF_003261095.1_ASM326109v1_genomic.fna/markers.fasta
[2024-01-24 14:06:44,941] [INFO] Task started: Blastn
[2024-01-24 14:06:44,941] [INFO] Running command: blastn -query GCF_003261095.1_ASM326109v1_genomic.fna/markers.fasta -db /var/lib/cwl/stga0a41cb8-7643-4c47-836a-29c376be3294/dqc_reference/reference_markers.fasta -out GCF_003261095.1_ASM326109v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:06:45,621] [INFO] Task succeeded: Blastn
[2024-01-24 14:06:45,625] [INFO] Selected 11 target genomes.
[2024-01-24 14:06:45,625] [INFO] Target genome list was writen to GCF_003261095.1_ASM326109v1_genomic.fna/target_genomes.txt
[2024-01-24 14:06:45,629] [INFO] Task started: fastANI
[2024-01-24 14:06:45,629] [INFO] Running command: fastANI --query /var/lib/cwl/stg04415fe0-1852-4926-a228-23f4913521e6/GCF_003261095.1_ASM326109v1_genomic.fna.gz --refList GCF_003261095.1_ASM326109v1_genomic.fna/target_genomes.txt --output GCF_003261095.1_ASM326109v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 14:06:58,052] [INFO] Task succeeded: fastANI
[2024-01-24 14:06:58,053] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stga0a41cb8-7643-4c47-836a-29c376be3294/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 14:06:58,054] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stga0a41cb8-7643-4c47-836a-29c376be3294/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 14:06:58,063] [INFO] Found 6 fastANI hits (3 hits with ANI > threshold)
[2024-01-24 14:06:58,064] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 14:06:58,064] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Desulfobacter hydrogenophilus	strain=DSM 3380	GCA_003261095.1	2291	2291	type	True	100.0	1594	1596	95	conclusive
Desulfobacter hydrogenophilus	strain=AcRS1	GCA_004319545.1	2291	2291	type	True	99.9951	1595	1596	95	conclusive
Desulfobacter hydrogenophilus	strain=DSM 3380	GCA_010499545.1	2291	2291	type	True	99.9891	1578	1596	95	conclusive
Desulfobacter curvatus	strain=DSM 3379	GCA_000373985.1	2290	2290	type	True	86.8757	1029	1596	95	below_threshold
Desulfobacter postgatei	strain=2ac9	GCA_000233695.3	2293	2293	type	True	86.1167	895	1596	95	below_threshold
Desulfobacter vibrioformis	strain=DSM 8776	GCA_000745975.1	34031	34031	type	True	86.0227	869	1596	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 14:06:58,065] [INFO] DFAST Taxonomy check result was written to GCF_003261095.1_ASM326109v1_genomic.fna/tc_result.tsv
[2024-01-24 14:06:58,066] [INFO] ===== Taxonomy check completed =====
[2024-01-24 14:06:58,066] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 14:06:58,067] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stga0a41cb8-7643-4c47-836a-29c376be3294/dqc_reference/checkm_data
[2024-01-24 14:06:58,068] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 14:06:58,117] [INFO] Task started: CheckM
[2024-01-24 14:06:58,117] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_003261095.1_ASM326109v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_003261095.1_ASM326109v1_genomic.fna/checkm_input GCF_003261095.1_ASM326109v1_genomic.fna/checkm_result
[2024-01-24 14:07:41,411] [INFO] Task succeeded: CheckM
[2024-01-24 14:07:41,413] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 14:07:41,436] [INFO] ===== Completeness check finished =====
[2024-01-24 14:07:41,436] [INFO] ===== Start GTDB Search =====
[2024-01-24 14:07:41,437] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_003261095.1_ASM326109v1_genomic.fna/markers.fasta)
[2024-01-24 14:07:41,437] [INFO] Task started: Blastn
[2024-01-24 14:07:41,437] [INFO] Running command: blastn -query GCF_003261095.1_ASM326109v1_genomic.fna/markers.fasta -db /var/lib/cwl/stga0a41cb8-7643-4c47-836a-29c376be3294/dqc_reference/reference_markers_gtdb.fasta -out GCF_003261095.1_ASM326109v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:07:42,208] [INFO] Task succeeded: Blastn
[2024-01-24 14:07:42,211] [INFO] Selected 7 target genomes.
[2024-01-24 14:07:42,212] [INFO] Target genome list was writen to GCF_003261095.1_ASM326109v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 14:07:42,217] [INFO] Task started: fastANI
[2024-01-24 14:07:42,218] [INFO] Running command: fastANI --query /var/lib/cwl/stg04415fe0-1852-4926-a228-23f4913521e6/GCF_003261095.1_ASM326109v1_genomic.fna.gz --refList GCF_003261095.1_ASM326109v1_genomic.fna/target_genomes_gtdb.txt --output GCF_003261095.1_ASM326109v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 14:07:50,569] [INFO] Task succeeded: fastANI
[2024-01-24 14:07:50,581] [INFO] Found 7 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 14:07:50,581] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_004319545.1	s__Desulfobacter hydrogenophilus	99.9951	1595	1596	d__Bacteria;p__Desulfobacterota;c__Desulfobacteria;o__Desulfobacterales;f__Desulfobacteraceae;g__Desulfobacter	95.0	99.99	99.99	1.00	1.00	3	conclusive
GCF_000373985.1	s__Desulfobacter curvatus	86.8819	1029	1596	d__Bacteria;p__Desulfobacterota;c__Desulfobacteria;o__Desulfobacterales;f__Desulfobacteraceae;g__Desulfobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCA_003252055.1	s__Desulfobacter postgatei_B	86.8011	586	1596	d__Bacteria;p__Desulfobacterota;c__Desulfobacteria;o__Desulfobacterales;f__Desulfobacteraceae;g__Desulfobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000233695.2	s__Desulfobacter postgatei	86.13	894	1596	d__Bacteria;p__Desulfobacterota;c__Desulfobacteria;o__Desulfobacterales;f__Desulfobacteraceae;g__Desulfobacter	95.0	96.23	96.02	0.82	0.77	7	-
GCF_013389365.1	s__Desulfobacter latus	86.043	825	1596	d__Bacteria;p__Desulfobacterota;c__Desulfobacteria;o__Desulfobacterales;f__Desulfobacteraceae;g__Desulfobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000745975.1	s__Desulfobacter vibrioformis	86.0043	872	1596	d__Bacteria;p__Desulfobacterota;c__Desulfobacteria;o__Desulfobacterales;f__Desulfobacteraceae;g__Desulfobacter	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002747145.1	s__Desulfobacter postgatei_A	84.5056	387	1596	d__Bacteria;p__Desulfobacterota;c__Desulfobacteria;o__Desulfobacterales;f__Desulfobacteraceae;g__Desulfobacter	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 14:07:50,583] [INFO] GTDB search result was written to GCF_003261095.1_ASM326109v1_genomic.fna/result_gtdb.tsv
[2024-01-24 14:07:50,583] [INFO] ===== GTDB Search completed =====
[2024-01-24 14:07:50,586] [INFO] DFAST_QC result json was written to GCF_003261095.1_ASM326109v1_genomic.fna/dqc_result.json
[2024-01-24 14:07:50,586] [INFO] DFAST_QC completed!
[2024-01-24 14:07:50,586] [INFO] Total running time: 0h1m21s