[2024-01-25 18:50:20,537] [INFO] DFAST_QC pipeline started.
[2024-01-25 18:50:20,538] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 18:50:20,538] [INFO] DQC Reference Directory: /var/lib/cwl/stg84430a99-5f4d-4c32-98a0-21e5a000451c/dqc_reference
[2024-01-25 18:50:21,639] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 18:50:21,640] [INFO] Task started: Prodigal
[2024-01-25 18:50:21,640] [INFO] Running command: gunzip -c /var/lib/cwl/stgc1365c33-1966-4e5f-a961-f1063f21d110/GCF_003324715.1_ASM332471v1_genomic.fna.gz | prodigal -d GCF_003324715.1_ASM332471v1_genomic.fna/cds.fna -a GCF_003324715.1_ASM332471v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 18:50:33,068] [INFO] Task succeeded: Prodigal
[2024-01-25 18:50:33,068] [INFO] Task started: HMMsearch
[2024-01-25 18:50:33,068] [INFO] Running command: hmmsearch --tblout GCF_003324715.1_ASM332471v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg84430a99-5f4d-4c32-98a0-21e5a000451c/dqc_reference/reference_markers.hmm GCF_003324715.1_ASM332471v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 18:50:33,341] [INFO] Task succeeded: HMMsearch
[2024-01-25 18:50:33,342] [INFO] Found 6/6 markers.
[2024-01-25 18:50:33,384] [INFO] Query marker FASTA was written to GCF_003324715.1_ASM332471v1_genomic.fna/markers.fasta
[2024-01-25 18:50:33,384] [INFO] Task started: Blastn
[2024-01-25 18:50:33,384] [INFO] Running command: blastn -query GCF_003324715.1_ASM332471v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg84430a99-5f4d-4c32-98a0-21e5a000451c/dqc_reference/reference_markers.fasta -out GCF_003324715.1_ASM332471v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 18:50:34,419] [INFO] Task succeeded: Blastn
[2024-01-25 18:50:34,421] [INFO] Selected 10 target genomes.
[2024-01-25 18:50:34,422] [INFO] Target genome list was writen to GCF_003324715.1_ASM332471v1_genomic.fna/target_genomes.txt
[2024-01-25 18:50:34,426] [INFO] Task started: fastANI
[2024-01-25 18:50:34,426] [INFO] Running command: fastANI --query /var/lib/cwl/stgc1365c33-1966-4e5f-a961-f1063f21d110/GCF_003324715.1_ASM332471v1_genomic.fna.gz --refList GCF_003324715.1_ASM332471v1_genomic.fna/target_genomes.txt --output GCF_003324715.1_ASM332471v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 18:50:44,659] [INFO] Task succeeded: fastANI
[2024-01-25 18:50:44,659] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg84430a99-5f4d-4c32-98a0-21e5a000451c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 18:50:44,660] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg84430a99-5f4d-4c32-98a0-21e5a000451c/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 18:50:44,667] [INFO] Found 10 fastANI hits (5 hits with ANI > threshold)
[2024-01-25 18:50:44,667] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-25 18:50:44,667] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Cereibacter sphaeroides	strain=2.4.1	GCA_003324715.1	1063	1063	type	True	100.0	1537	1540	95	conclusive
Cereibacter sphaeroides	strain=2.4.1	GCA_000273405.1	1063	1063	type	True	99.9918	1531	1540	95	conclusive
Cereibacter sphaeroides	strain=2.4.1	GCA_000012905.2	1063	1063	type	True	99.9879	1526	1540	95	conclusive
Cereibacter sphaeroides	strain=2.4.1	GCA_000269625.2	1063	1063	type	True	99.9863	1525	1540	95	conclusive
Cereibacter sphaeroides	strain=NBRC 12203	GCA_007991035.1	1063	1063	type	True	99.9549	1455	1540	95	conclusive
Cereibacter sediminicola	strain=JA983	GCA_007668225.1	2584941	2584941	type	True	87.1432	1012	1540	95	below_threshold
Cereibacter azotoformans	strain=KA25	GCA_003050905.1	43057	43057	type	True	87.0592	1060	1540	95	below_threshold
Cereibacter ovatus	strain=JA234	GCA_900207575.1	439529	439529	type	True	82.8577	818	1540	95	below_threshold
Rhodobacter calidifons	strain=M37P	GCA_011174775.1	2715277	2715277	type	True	79.2779	555	1540	95	below_threshold
Gemmobacter fulva	strain=con5	GCA_018798885.1	2840474	2840474	type	True	78.7109	511	1540	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 18:50:44,672] [INFO] DFAST Taxonomy check result was written to GCF_003324715.1_ASM332471v1_genomic.fna/tc_result.tsv
[2024-01-25 18:50:44,673] [INFO] ===== Taxonomy check completed =====
[2024-01-25 18:50:44,673] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 18:50:44,673] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg84430a99-5f4d-4c32-98a0-21e5a000451c/dqc_reference/checkm_data
[2024-01-25 18:50:44,674] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 18:50:44,719] [INFO] Task started: CheckM
[2024-01-25 18:50:44,719] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_003324715.1_ASM332471v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_003324715.1_ASM332471v1_genomic.fna/checkm_input GCF_003324715.1_ASM332471v1_genomic.fna/checkm_result
[2024-01-25 18:51:41,993] [INFO] Task succeeded: CheckM
[2024-01-25 18:51:41,994] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 18:51:42,012] [INFO] ===== Completeness check finished =====
[2024-01-25 18:51:42,012] [INFO] ===== Start GTDB Search =====
[2024-01-25 18:51:42,013] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_003324715.1_ASM332471v1_genomic.fna/markers.fasta)
[2024-01-25 18:51:42,013] [INFO] Task started: Blastn
[2024-01-25 18:51:42,013] [INFO] Running command: blastn -query GCF_003324715.1_ASM332471v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg84430a99-5f4d-4c32-98a0-21e5a000451c/dqc_reference/reference_markers_gtdb.fasta -out GCF_003324715.1_ASM332471v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 18:51:44,133] [INFO] Task succeeded: Blastn
[2024-01-25 18:51:44,136] [INFO] Selected 6 target genomes.
[2024-01-25 18:51:44,136] [INFO] Target genome list was writen to GCF_003324715.1_ASM332471v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 18:51:44,143] [INFO] Task started: fastANI
[2024-01-25 18:51:44,143] [INFO] Running command: fastANI --query /var/lib/cwl/stgc1365c33-1966-4e5f-a961-f1063f21d110/GCF_003324715.1_ASM332471v1_genomic.fna.gz --refList GCF_003324715.1_ASM332471v1_genomic.fna/target_genomes_gtdb.txt --output GCF_003324715.1_ASM332471v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 18:51:51,077] [INFO] Task succeeded: fastANI
[2024-01-25 18:51:51,082] [INFO] Found 6 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 18:51:51,082] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_000012905.2	s__Cereibacter_A sphaeroides	99.9879	1527	1540	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhodobacterales;f__Rhodobacteraceae;g__Cereibacter_A	95.1736	98.82	96.57	0.94	0.87	24	conclusive
GCF_001720585.1	s__Cereibacter_A johrii	95.0975	1156	1540	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhodobacterales;f__Rhodobacteraceae;g__Cereibacter_A	95.1736	98.42	97.86	0.94	0.91	6	-
GCA_017599285.1	s__Cereibacter_A azotoformans	87.5067	887	1540	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhodobacterales;f__Rhodobacteraceae;g__Cereibacter_A	95.0	98.54	95.54	0.92	0.86	6	-
GCF_002407205.1	s__Cereibacter_A sp002407205	84.8821	1008	1540	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhodobacterales;f__Rhodobacteraceae;g__Cereibacter_A	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900207575.1	s__Cereibacter_A ovatus	82.8375	820	1540	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhodobacterales;f__Rhodobacteraceae;g__Cereibacter_A	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003034985.1	s__Cereibacter changlensis	82.5197	818	1540	d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Rhodobacterales;f__Rhodobacteraceae;g__Cereibacter	95.0	98.07	96.18	0.89	0.78	3	-
--------------------------------------------------------------------------------
[2024-01-25 18:51:51,084] [INFO] GTDB search result was written to GCF_003324715.1_ASM332471v1_genomic.fna/result_gtdb.tsv
[2024-01-25 18:51:51,084] [INFO] ===== GTDB Search completed =====
[2024-01-25 18:51:51,086] [INFO] DFAST_QC result json was written to GCF_003324715.1_ASM332471v1_genomic.fna/dqc_result.json
[2024-01-25 18:51:51,087] [INFO] DFAST_QC completed!
[2024-01-25 18:51:51,087] [INFO] Total running time: 0h1m31s