[2024-01-24 12:40:08,140] [INFO] DFAST_QC pipeline started.
[2024-01-24 12:40:08,142] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 12:40:08,143] [INFO] DQC Reference Directory: /var/lib/cwl/stge634a71a-eca4-4da7-83e1-e4e057a00f10/dqc_reference
[2024-01-24 12:40:09,619] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 12:40:09,644] [INFO] Task started: Prodigal
[2024-01-24 12:40:09,645] [INFO] Running command: gunzip -c /var/lib/cwl/stg0f1568cb-3c1d-4343-b4ab-4daea8d9e059/GCF_003431655.1_ASM343165v1_genomic.fna.gz | prodigal -d GCF_003431655.1_ASM343165v1_genomic.fna/cds.fna -a GCF_003431655.1_ASM343165v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 12:40:30,796] [INFO] Task succeeded: Prodigal
[2024-01-24 12:40:30,796] [INFO] Task started: HMMsearch
[2024-01-24 12:40:30,796] [INFO] Running command: hmmsearch --tblout GCF_003431655.1_ASM343165v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stge634a71a-eca4-4da7-83e1-e4e057a00f10/dqc_reference/reference_markers.hmm GCF_003431655.1_ASM343165v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 12:40:31,099] [INFO] Task succeeded: HMMsearch
[2024-01-24 12:40:31,100] [INFO] Found 6/6 markers.
[2024-01-24 12:40:31,153] [INFO] Query marker FASTA was written to GCF_003431655.1_ASM343165v1_genomic.fna/markers.fasta
[2024-01-24 12:40:31,154] [INFO] Task started: Blastn
[2024-01-24 12:40:31,154] [INFO] Running command: blastn -query GCF_003431655.1_ASM343165v1_genomic.fna/markers.fasta -db /var/lib/cwl/stge634a71a-eca4-4da7-83e1-e4e057a00f10/dqc_reference/reference_markers.fasta -out GCF_003431655.1_ASM343165v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:40:32,265] [INFO] Task succeeded: Blastn
[2024-01-24 12:40:32,269] [INFO] Selected 7 target genomes.
[2024-01-24 12:40:32,270] [INFO] Target genome list was writen to GCF_003431655.1_ASM343165v1_genomic.fna/target_genomes.txt
[2024-01-24 12:40:32,275] [INFO] Task started: fastANI
[2024-01-24 12:40:32,275] [INFO] Running command: fastANI --query /var/lib/cwl/stg0f1568cb-3c1d-4343-b4ab-4daea8d9e059/GCF_003431655.1_ASM343165v1_genomic.fna.gz --refList GCF_003431655.1_ASM343165v1_genomic.fna/target_genomes.txt --output GCF_003431655.1_ASM343165v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 12:40:47,666] [INFO] Task succeeded: fastANI
[2024-01-24 12:40:47,667] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stge634a71a-eca4-4da7-83e1-e4e057a00f10/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 12:40:47,667] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stge634a71a-eca4-4da7-83e1-e4e057a00f10/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 12:40:47,679] [INFO] Found 7 fastANI hits (7 hits with ANI > threshold)
[2024-01-24 12:40:47,679] [INFO] The taxonomy check result is classified as 'inconclusive'.
[2024-01-24 12:40:47,679] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Mycobacterium marinum	strain=NCTC2275	GCA_003431655.1	1781	1781	suspected-type	True	100.0	2027	2032	95	inconclusive
Mycobacterium marinum	strain=DSM 44344	GCA_003431645.1	1781	1781	suspected-type	True	99.8878	1992	2032	95	inconclusive
Mycobacterium marinum	strain=CCUG20998	GCA_003391395.1	1781	1781	suspected-type	True	99.8833	2007	2032	95	inconclusive
Mycobacterium shottsii	strain=M175	GCA_019456695.2	133549	133549	suspected-type	True	98.707	1761	2032	95	inconclusive
Mycobacterium shottsii	strain=JCM 12657	GCA_010728525.1	133549	133549	suspected-type	True	98.6732	1773	2032	95	inconclusive
Mycobacterium pseudoshottsii	strain=JCM 15466	GCA_001515365.1	265949	265949	suspected-type	True	98.0458	1750	2032	95	inconclusive
Mycobacterium pseudoshottsii	strain=JCM 15466	GCA_003584745.1	265949	265949	suspected-type	True	97.9087	1861	2032	95	inconclusive
--------------------------------------------------------------------------------
[2024-01-24 12:40:47,680] [INFO] DFAST Taxonomy check result was written to GCF_003431655.1_ASM343165v1_genomic.fna/tc_result.tsv
[2024-01-24 12:40:47,681] [INFO] ===== Taxonomy check completed =====
[2024-01-24 12:40:47,681] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 12:40:47,681] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stge634a71a-eca4-4da7-83e1-e4e057a00f10/dqc_reference/checkm_data
[2024-01-24 12:40:47,682] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 12:40:47,744] [INFO] Task started: CheckM
[2024-01-24 12:40:47,744] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_003431655.1_ASM343165v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_003431655.1_ASM343165v1_genomic.fna/checkm_input GCF_003431655.1_ASM343165v1_genomic.fna/checkm_result
[2024-01-24 12:41:44,188] [INFO] Task succeeded: CheckM
[2024-01-24 12:41:44,189] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 12:41:44,213] [INFO] ===== Completeness check finished =====
[2024-01-24 12:41:44,214] [INFO] ===== Start GTDB Search =====
[2024-01-24 12:41:44,214] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_003431655.1_ASM343165v1_genomic.fna/markers.fasta)
[2024-01-24 12:41:44,215] [INFO] Task started: Blastn
[2024-01-24 12:41:44,215] [INFO] Running command: blastn -query GCF_003431655.1_ASM343165v1_genomic.fna/markers.fasta -db /var/lib/cwl/stge634a71a-eca4-4da7-83e1-e4e057a00f10/dqc_reference/reference_markers_gtdb.fasta -out GCF_003431655.1_ASM343165v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 12:41:45,750] [INFO] Task succeeded: Blastn
[2024-01-24 12:41:45,755] [INFO] Selected 18 target genomes.
[2024-01-24 12:41:45,755] [INFO] Target genome list was writen to GCF_003431655.1_ASM343165v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 12:41:45,766] [INFO] Task started: fastANI
[2024-01-24 12:41:45,767] [INFO] Running command: fastANI --query /var/lib/cwl/stg0f1568cb-3c1d-4343-b4ab-4daea8d9e059/GCF_003431655.1_ASM343165v1_genomic.fna.gz --refList GCF_003431655.1_ASM343165v1_genomic.fna/target_genomes_gtdb.txt --output GCF_003431655.1_ASM343165v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 12:42:13,077] [INFO] Task succeeded: fastANI
[2024-01-24 12:42:13,096] [INFO] Found 18 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 12:42:13,097] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_003391395.1	s__Mycobacterium marinum	99.8833	2007	2032	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	98.78	97.50	0.95	0.86	40	conclusive
GCF_900566055.1	s__Mycobacterium innocens	81.1582	1148	2032	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	99.27	98.57	0.94	0.90	3	-
GCF_900292015.1	s__Mycobacterium basiliense	81.0976	1285	2032	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002705925.1	s__Mycobacterium ostraviense	81.0595	1201	2032	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	99.87	99.87	0.99	0.99	2	-
GCF_002102175.1	s__Mycobacterium gastri	81.0151	1177	2032	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	99.95	99.95	0.95	0.95	2	-
GCF_900566075.1	s__Mycobacterium pseudokansasii	80.9681	1193	2032	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	99.70	99.48	0.96	0.94	7	-
GCF_000157895.3	s__Mycobacterium kansasii	80.9336	1227	2032	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	99.70	99.15	0.98	0.95	35	-
GCF_002086675.1	s__Mycobacterium persicum	80.8158	1155	2032	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	99.71	99.58	0.97	0.95	12	-
GCF_002086155.1	s__Mycobacterium angelicum	80.3448	1091	2032	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002104675.1	s__Mycobacterium decipiens	80.2673	1037	2032	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCA_002102225.1	s__Mycobacterium interjectum_B	80.1533	1006	2032	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003614435.1	s__Mycobacterium paragordonae	79.9857	1041	2032	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	98.23	97.83	0.93	0.88	9	-
GCF_001417955.2	s__Mycobacterium gordonae_B	79.9082	1021	2032	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_015689175.1	s__Mycobacterium kubicae	79.8707	927	2032	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	99.05	97.98	0.96	0.92	7	-
GCF_017086405.1	s__Mycobacterium gordonae_D	79.687	1018	2032	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	N/A	N/A	N/A	N/A	1	-
GCF_002101675.1	s__Mycobacterium gordonae	79.6782	1019	2032	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	99.22	98.97	0.90	0.87	6	-
GCF_010729105.1	s__Mycobacterium saskatchewanense	79.6121	920	2032	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	99.98	99.98	1.00	1.00	2	-
GCF_003112775.1	s__Mycobacterium montefiorense	79.4482	900	2032	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Mycobacteriales;f__Mycobacteriaceae;g__Mycobacterium	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 12:42:13,100] [INFO] GTDB search result was written to GCF_003431655.1_ASM343165v1_genomic.fna/result_gtdb.tsv
[2024-01-24 12:42:13,100] [INFO] ===== GTDB Search completed =====
[2024-01-24 12:42:13,104] [INFO] DFAST_QC result json was written to GCF_003431655.1_ASM343165v1_genomic.fna/dqc_result.json
[2024-01-24 12:42:13,104] [INFO] DFAST_QC completed!
[2024-01-24 12:42:13,104] [INFO] Total running time: 0h2m5s