[2024-01-24 13:01:39,625] [INFO] DFAST_QC pipeline started.
[2024-01-24 13:01:39,630] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 13:01:39,630] [INFO] DQC Reference Directory: /var/lib/cwl/stgdbf985eb-fbbf-45f1-a40e-48f2f3efeb66/dqc_reference
[2024-01-24 13:01:41,369] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 13:01:41,369] [INFO] Task started: Prodigal
[2024-01-24 13:01:41,370] [INFO] Running command: gunzip -c /var/lib/cwl/stg80f4988a-639c-4805-864e-b2034b84c50d/GCF_003576405.1_ASM357640v1_genomic.fna.gz | prodigal -d GCF_003576405.1_ASM357640v1_genomic.fna/cds.fna -a GCF_003576405.1_ASM357640v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 13:01:48,616] [INFO] Task succeeded: Prodigal
[2024-01-24 13:01:48,616] [INFO] Task started: HMMsearch
[2024-01-24 13:01:48,616] [INFO] Running command: hmmsearch --tblout GCF_003576405.1_ASM357640v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgdbf985eb-fbbf-45f1-a40e-48f2f3efeb66/dqc_reference/reference_markers.hmm GCF_003576405.1_ASM357640v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 13:01:48,892] [INFO] Task succeeded: HMMsearch
[2024-01-24 13:01:48,893] [INFO] Found 6/6 markers.
[2024-01-24 13:01:48,926] [INFO] Query marker FASTA was written to GCF_003576405.1_ASM357640v1_genomic.fna/markers.fasta
[2024-01-24 13:01:48,926] [INFO] Task started: Blastn
[2024-01-24 13:01:48,926] [INFO] Running command: blastn -query GCF_003576405.1_ASM357640v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgdbf985eb-fbbf-45f1-a40e-48f2f3efeb66/dqc_reference/reference_markers.fasta -out GCF_003576405.1_ASM357640v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:01:50,241] [INFO] Task succeeded: Blastn
[2024-01-24 13:01:50,245] [INFO] Selected 11 target genomes.
[2024-01-24 13:01:50,245] [INFO] Target genome list was writen to GCF_003576405.1_ASM357640v1_genomic.fna/target_genomes.txt
[2024-01-24 13:01:50,251] [INFO] Task started: fastANI
[2024-01-24 13:01:50,252] [INFO] Running command: fastANI --query /var/lib/cwl/stg80f4988a-639c-4805-864e-b2034b84c50d/GCF_003576405.1_ASM357640v1_genomic.fna.gz --refList GCF_003576405.1_ASM357640v1_genomic.fna/target_genomes.txt --output GCF_003576405.1_ASM357640v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 13:01:58,775] [INFO] Task succeeded: fastANI
[2024-01-24 13:01:58,776] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgdbf985eb-fbbf-45f1-a40e-48f2f3efeb66/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 13:01:58,776] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgdbf985eb-fbbf-45f1-a40e-48f2f3efeb66/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 13:01:58,786] [INFO] Found 11 fastANI hits (0 hits with ANI > threshold)
[2024-01-24 13:01:58,786] [INFO] The taxonomy check result is classified as 'below_threshold'.
[2024-01-24 13:01:58,786] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Clavibacter phaseoli	strain=LPPA 982	GCA_021922925.1	1734031	1734031	type	True	93.0759	723	789	95	below_threshold
Clavibacter michiganensis	strain=VKM Ac-1403	GCA_900168345.1	28447	28447	suspected-type	True	92.765	701	789	95	below_threshold
Clavibacter michiganensis subsp. insidiosus	strain=CFBP 2404	GCA_003693415.1	33014	28447	type	True	92.6704	689	789	95	below_threshold
Clavibacter michiganensis subsp. michiganensis	strain=LMG 7333	GCA_002240575.1	33013	28447	type	True	92.6441	712	789	95	below_threshold
Clavibacter michiganensis	strain=LMG7333	GCA_021216655.1	28447	28447	suspected-type	True	92.6081	717	789	95	below_threshold
Clavibacter michiganensis subsp. insidiosus	strain=LMG 3663	GCA_002240565.1	33014	28447	type	True	92.5992	718	789	95	below_threshold
Clavibacter michiganensis subsp. insidiosus	strain=ATCC 10253	GCA_003076355.1	33014	28447	type	True	92.5883	714	789	95	below_threshold
Clavibacter californiensis	strain=CFBP 8216	GCA_003576155.1	1401995	1401995	type	True	92.4909	552	789	95	below_threshold
Clavibacter californiensis	strain=CFBP 8216	GCA_021952865.1	1401995	1401995	type	True	92.3832	709	789	95	below_threshold
Clavibacter michiganensis subsp. tessellarius	strain=ATCC 33566	GCA_002240635.1	31965	28447	type	True	91.2636	694	789	95	below_threshold
Clavibacter michiganensis subsp. tessellarius	strain=ATCC 33566	GCA_021922985.1	31965	28447	type	True	91.2406	715	789	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 13:01:58,789] [INFO] DFAST Taxonomy check result was written to GCF_003576405.1_ASM357640v1_genomic.fna/tc_result.tsv
[2024-01-24 13:01:58,790] [INFO] ===== Taxonomy check completed =====
[2024-01-24 13:01:58,790] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 13:01:58,790] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgdbf985eb-fbbf-45f1-a40e-48f2f3efeb66/dqc_reference/checkm_data
[2024-01-24 13:01:58,791] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 13:01:58,826] [INFO] Task started: CheckM
[2024-01-24 13:01:58,826] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_003576405.1_ASM357640v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_003576405.1_ASM357640v1_genomic.fna/checkm_input GCF_003576405.1_ASM357640v1_genomic.fna/checkm_result
[2024-01-24 13:02:27,186] [INFO] Task succeeded: CheckM
[2024-01-24 13:02:27,188] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 13:02:27,214] [INFO] ===== Completeness check finished =====
[2024-01-24 13:02:27,215] [INFO] ===== Start GTDB Search =====
[2024-01-24 13:02:27,215] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_003576405.1_ASM357640v1_genomic.fna/markers.fasta)
[2024-01-24 13:02:27,216] [INFO] Task started: Blastn
[2024-01-24 13:02:27,216] [INFO] Running command: blastn -query GCF_003576405.1_ASM357640v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgdbf985eb-fbbf-45f1-a40e-48f2f3efeb66/dqc_reference/reference_markers_gtdb.fasta -out GCF_003576405.1_ASM357640v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 13:02:29,365] [INFO] Task succeeded: Blastn
[2024-01-24 13:02:29,370] [INFO] Selected 12 target genomes.
[2024-01-24 13:02:29,370] [INFO] Target genome list was writen to GCF_003576405.1_ASM357640v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 13:02:29,380] [INFO] Task started: fastANI
[2024-01-24 13:02:29,380] [INFO] Running command: fastANI --query /var/lib/cwl/stg80f4988a-639c-4805-864e-b2034b84c50d/GCF_003576405.1_ASM357640v1_genomic.fna.gz --refList GCF_003576405.1_ASM357640v1_genomic.fna/target_genomes_gtdb.txt --output GCF_003576405.1_ASM357640v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 13:02:38,200] [INFO] Task succeeded: fastANI
[2024-01-24 13:02:38,215] [INFO] Found 12 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 13:02:38,215] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_003576405.1	s__Clavibacter sp003576405	100.0	774	789	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Microbacteriaceae;g__Clavibacter	95.0	99.88	99.88	0.93	0.93	2	conclusive
GCF_002151125.1	s__Clavibacter michiganensis_C	93.3257	720	789	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Microbacteriaceae;g__Clavibacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003576185.1	s__Clavibacter phaseoli	92.9306	546	789	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Microbacteriaceae;g__Clavibacter	95.0	96.97	95.69	0.87	0.85	4	-
GCF_016907085.1	s__Clavibacter michiganensis_O	92.8143	709	789	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Microbacteriaceae;g__Clavibacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900168345.1	s__Clavibacter michiganensis	92.7261	703	789	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Microbacteriaceae;g__Clavibacter	95.0	99.05	95.09	0.97	0.88	49	-
GCF_000355695.1	s__Clavibacter nebraskensis	92.6225	699	789	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Microbacteriaceae;g__Clavibacter	95.1408	99.97	99.95	1.00	1.00	6	-
GCF_002240565.1	s__Clavibacter insidiosus	92.6188	717	789	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Microbacteriaceae;g__Clavibacter	95.1408	99.26	95.88	0.97	0.89	7	-
GCF_002931175.1	s__Clavibacter californiensis	92.4283	701	789	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Microbacteriaceae;g__Clavibacter	95.0	98.71	98.71	0.95	0.95	2	-
GCF_000069225.1	s__Clavibacter sepedonicus	92.2414	679	789	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Microbacteriaceae;g__Clavibacter	95.0	99.98	99.98	0.98	0.96	4	-
GCF_003384945.1	s__Clavibacter sp003384945	91.9991	715	789	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Microbacteriaceae;g__Clavibacter	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001280205.1	s__Clavibacter capsici	91.8173	708	789	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Microbacteriaceae;g__Clavibacter	95.0	98.16	95.31	0.96	0.91	6	-
GCF_002240635.1	s__Clavibacter tessellarius	91.2157	696	789	d__Bacteria;p__Actinobacteriota;c__Actinomycetia;o__Actinomycetales;f__Microbacteriaceae;g__Clavibacter	95.0	96.95	95.37	0.96	0.94	3	-
--------------------------------------------------------------------------------
[2024-01-24 13:02:38,217] [INFO] GTDB search result was written to GCF_003576405.1_ASM357640v1_genomic.fna/result_gtdb.tsv
[2024-01-24 13:02:38,217] [INFO] ===== GTDB Search completed =====
[2024-01-24 13:02:38,221] [INFO] DFAST_QC result json was written to GCF_003576405.1_ASM357640v1_genomic.fna/dqc_result.json
[2024-01-24 13:02:38,221] [INFO] DFAST_QC completed!
[2024-01-24 13:02:38,221] [INFO] Total running time: 0h0m59s