[2024-01-24 10:47:06,547] [INFO] DFAST_QC pipeline started.
[2024-01-24 10:47:06,552] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 10:47:06,552] [INFO] DQC Reference Directory: /var/lib/cwl/stg9ab719b1-837e-48ca-9cff-89bccf20b0ca/dqc_reference
[2024-01-24 10:47:12,100] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 10:47:12,102] [INFO] Task started: Prodigal
[2024-01-24 10:47:12,102] [INFO] Running command: gunzip -c /var/lib/cwl/stg03a85c0c-60d2-4d44-889e-faf6d7a25dfd/GCF_003946175.1_ASM394617v1_genomic.fna.gz | prodigal -d GCF_003946175.1_ASM394617v1_genomic.fna/cds.fna -a GCF_003946175.1_ASM394617v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 10:47:19,618] [INFO] Task succeeded: Prodigal
[2024-01-24 10:47:19,618] [INFO] Task started: HMMsearch
[2024-01-24 10:47:19,619] [INFO] Running command: hmmsearch --tblout GCF_003946175.1_ASM394617v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg9ab719b1-837e-48ca-9cff-89bccf20b0ca/dqc_reference/reference_markers.hmm GCF_003946175.1_ASM394617v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 10:47:19,867] [INFO] Task succeeded: HMMsearch
[2024-01-24 10:47:19,869] [INFO] Found 6/6 markers.
[2024-01-24 10:47:19,894] [INFO] Query marker FASTA was written to GCF_003946175.1_ASM394617v1_genomic.fna/markers.fasta
[2024-01-24 10:47:19,895] [INFO] Task started: Blastn
[2024-01-24 10:47:19,895] [INFO] Running command: blastn -query GCF_003946175.1_ASM394617v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg9ab719b1-837e-48ca-9cff-89bccf20b0ca/dqc_reference/reference_markers.fasta -out GCF_003946175.1_ASM394617v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 10:47:20,562] [INFO] Task succeeded: Blastn
[2024-01-24 10:47:20,568] [INFO] Selected 26 target genomes.
[2024-01-24 10:47:20,569] [INFO] Target genome list was writen to GCF_003946175.1_ASM394617v1_genomic.fna/target_genomes.txt
[2024-01-24 10:47:20,661] [INFO] Task started: fastANI
[2024-01-24 10:47:20,661] [INFO] Running command: fastANI --query /var/lib/cwl/stg03a85c0c-60d2-4d44-889e-faf6d7a25dfd/GCF_003946175.1_ASM394617v1_genomic.fna.gz --refList GCF_003946175.1_ASM394617v1_genomic.fna/target_genomes.txt --output GCF_003946175.1_ASM394617v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 10:47:34,145] [INFO] Task succeeded: fastANI
[2024-01-24 10:47:34,145] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg9ab719b1-837e-48ca-9cff-89bccf20b0ca/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 10:47:34,146] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg9ab719b1-837e-48ca-9cff-89bccf20b0ca/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 10:47:34,154] [INFO] Found 9 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 10:47:34,154] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 10:47:34,155] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Lacticaseibacillus suibinensis	strain=247-3	GCA_003946175.1	2486011	2486011	type	True	100.0	824	826	95	conclusive
Lacticaseibacillus suilingensis	strain=ZW152	GCA_016861845.1	2799577	2799577	type	True	92.2879	714	826	95	below_threshold
Lacticaseibacillus camelliae	strain=JCM 13995	GCA_001311195.1	381742	381742	type	True	79.4206	245	826	95	below_threshold
Lacticaseibacillus camelliae	strain=DSM 22697	GCA_001436615.1	381742	381742	type	True	79.362	257	826	95	below_threshold
Lacticaseibacillus hegangensis	strain=73-4	GCA_003946465.1	2486010	2486010	type	True	79.0454	263	826	95	below_threshold
Lacticaseibacillus jixianensis	strain=159-4	GCA_003946485.1	2486012	2486012	type	True	78.6674	236	826	95	below_threshold
Lacticaseibacillus daqingensis	strain=143-4(a)	GCA_003946115.1	2486014	2486014	type	True	78.5842	183	826	95	below_threshold
Lacticaseibacillus nasuensis	strain=JCM 17158	GCA_001434705.1	944671	944671	type	True	77.9884	153	826	95	below_threshold
Lacticaseibacillus sharpeae	strain=JCM 1186	GCA_001311055.1	1626	1626	type	True	77.4384	69	826	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 10:47:34,156] [INFO] DFAST Taxonomy check result was written to GCF_003946175.1_ASM394617v1_genomic.fna/tc_result.tsv
[2024-01-24 10:47:34,157] [INFO] ===== Taxonomy check completed =====
[2024-01-24 10:47:34,157] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 10:47:34,157] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg9ab719b1-837e-48ca-9cff-89bccf20b0ca/dqc_reference/checkm_data
[2024-01-24 10:47:34,158] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 10:47:34,185] [INFO] Task started: CheckM
[2024-01-24 10:47:34,185] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_003946175.1_ASM394617v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_003946175.1_ASM394617v1_genomic.fna/checkm_input GCF_003946175.1_ASM394617v1_genomic.fna/checkm_result
[2024-01-24 10:48:01,789] [INFO] Task succeeded: CheckM
[2024-01-24 10:48:01,795] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 10:48:01,818] [INFO] ===== Completeness check finished =====
[2024-01-24 10:48:01,818] [INFO] ===== Start GTDB Search =====
[2024-01-24 10:48:01,818] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_003946175.1_ASM394617v1_genomic.fna/markers.fasta)
[2024-01-24 10:48:01,819] [INFO] Task started: Blastn
[2024-01-24 10:48:01,819] [INFO] Running command: blastn -query GCF_003946175.1_ASM394617v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg9ab719b1-837e-48ca-9cff-89bccf20b0ca/dqc_reference/reference_markers_gtdb.fasta -out GCF_003946175.1_ASM394617v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 10:48:02,707] [INFO] Task succeeded: Blastn
[2024-01-24 10:48:02,711] [INFO] Selected 20 target genomes.
[2024-01-24 10:48:02,711] [INFO] Target genome list was writen to GCF_003946175.1_ASM394617v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 10:48:02,734] [INFO] Task started: fastANI
[2024-01-24 10:48:02,734] [INFO] Running command: fastANI --query /var/lib/cwl/stg03a85c0c-60d2-4d44-889e-faf6d7a25dfd/GCF_003946175.1_ASM394617v1_genomic.fna.gz --refList GCF_003946175.1_ASM394617v1_genomic.fna/target_genomes_gtdb.txt --output GCF_003946175.1_ASM394617v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 10:48:11,607] [INFO] Task succeeded: fastANI
[2024-01-24 10:48:11,616] [INFO] Found 10 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 10:48:11,616] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_003946175.1	s__Lacticaseibacillus suibinensis	100.0	824	826	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lacticaseibacillus	95.0	98.05	98.05	0.90	0.90	2	conclusive
GCF_016861845.1	s__Lacticaseibacillus sp900540605	92.3094	713	826	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lacticaseibacillus	95.0	99.61	99.51	0.94	0.94	3	-
GCF_003946165.1	s__Lacticaseibacillus baoqingensis	79.4151	138	826	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lacticaseibacillus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001311195.1	s__Lacticaseibacillus camelliae	79.3532	249	826	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lacticaseibacillus	95.0	99.89	99.89	1.00	1.00	2	-
GCF_003946465.1	s__Lacticaseibacillus hegangensis	79.0828	264	826	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lacticaseibacillus	95.0	95.70	95.61	0.83	0.83	3	-
GCF_003946485.1	s__Lacticaseibacillus jixianensis	78.704	235	826	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lacticaseibacillus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_003946115.1	s__Lacticaseibacillus daqingensis	78.5842	183	826	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lacticaseibacillus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_001435035.1	s__Lacticaseibacillus manihotivorans	78.3366	139	826	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lacticaseibacillus	95.0	99.87	99.83	0.97	0.96	3	-
GCF_001434705.1	s__Lacticaseibacillus nasuensis	77.9826	152	826	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lacticaseibacillus	95.0	99.88	99.88	1.00	1.00	2	-
GCF_003946405.1	s__Lacticaseibacillus yichunensis	77.8921	143	826	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Lactobacillaceae;g__Lacticaseibacillus	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 10:48:11,618] [INFO] GTDB search result was written to GCF_003946175.1_ASM394617v1_genomic.fna/result_gtdb.tsv
[2024-01-24 10:48:11,618] [INFO] ===== GTDB Search completed =====
[2024-01-24 10:48:11,621] [INFO] DFAST_QC result json was written to GCF_003946175.1_ASM394617v1_genomic.fna/dqc_result.json
[2024-01-24 10:48:11,621] [INFO] DFAST_QC completed!
[2024-01-24 10:48:11,621] [INFO] Total running time: 0h1m5s