[2024-01-25 18:16:20,650] [INFO] DFAST_QC pipeline started.
[2024-01-25 18:16:20,652] [INFO] DFAST_QC version: 0.5.7
[2024-01-25 18:16:20,652] [INFO] DQC Reference Directory: /var/lib/cwl/stg72976acc-0a84-45d3-b399-661bd0ac590f/dqc_reference
[2024-01-25 18:16:21,802] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-25 18:16:21,804] [INFO] Task started: Prodigal
[2024-01-25 18:16:21,804] [INFO] Running command: gunzip -c /var/lib/cwl/stga516cc9a-9f75-4ea0-92e3-351fb59007a7/GCF_003970555.1_ASM397055v1_genomic.fna.gz | prodigal -d GCF_003970555.1_ASM397055v1_genomic.fna/cds.fna -a GCF_003970555.1_ASM397055v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-25 18:16:26,200] [INFO] Task succeeded: Prodigal
[2024-01-25 18:16:26,200] [INFO] Task started: HMMsearch
[2024-01-25 18:16:26,200] [INFO] Running command: hmmsearch --tblout GCF_003970555.1_ASM397055v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stg72976acc-0a84-45d3-b399-661bd0ac590f/dqc_reference/reference_markers.hmm GCF_003970555.1_ASM397055v1_genomic.fna/protein.faa > /dev/null
[2024-01-25 18:16:26,430] [INFO] Task succeeded: HMMsearch
[2024-01-25 18:16:26,431] [INFO] Found 6/6 markers.
[2024-01-25 18:16:26,454] [INFO] Query marker FASTA was written to GCF_003970555.1_ASM397055v1_genomic.fna/markers.fasta
[2024-01-25 18:16:26,455] [INFO] Task started: Blastn
[2024-01-25 18:16:26,455] [INFO] Running command: blastn -query GCF_003970555.1_ASM397055v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg72976acc-0a84-45d3-b399-661bd0ac590f/dqc_reference/reference_markers.fasta -out GCF_003970555.1_ASM397055v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 18:16:27,040] [INFO] Task succeeded: Blastn
[2024-01-25 18:16:27,043] [INFO] Selected 12 target genomes.
[2024-01-25 18:16:27,043] [INFO] Target genome list was writen to GCF_003970555.1_ASM397055v1_genomic.fna/target_genomes.txt
[2024-01-25 18:16:27,050] [INFO] Task started: fastANI
[2024-01-25 18:16:27,050] [INFO] Running command: fastANI --query /var/lib/cwl/stga516cc9a-9f75-4ea0-92e3-351fb59007a7/GCF_003970555.1_ASM397055v1_genomic.fna.gz --refList GCF_003970555.1_ASM397055v1_genomic.fna/target_genomes.txt --output GCF_003970555.1_ASM397055v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-25 18:16:34,042] [INFO] Task succeeded: fastANI
[2024-01-25 18:16:34,043] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stg72976acc-0a84-45d3-b399-661bd0ac590f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-25 18:16:34,043] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stg72976acc-0a84-45d3-b399-661bd0ac590f/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-25 18:16:34,050] [INFO] Found 9 fastANI hits (2 hits with ANI > threshold)
[2024-01-25 18:16:34,050] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-25 18:16:34,050] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Staphylococcus piscifermentans	strain=DSM 7373	GCA_003970555.1	70258	70258	type	True	100.0	812	818	95	conclusive
Staphylococcus piscifermentans	strain=NCTC13836	GCA_900186985.1	70258	70258	type	True	99.9977	809	818	95	conclusive
Staphylococcus debuckii	strain=SDB 2975	GCA_003718735.1	2044912	2044912	type	True	94.5736	751	818	95	below_threshold
Staphylococcus carnosus	strain=NCTC13825	GCA_900458435.1	1281	1281	type	True	85.0824	668	818	95	below_threshold
Staphylococcus carnosus	strain=FDAARGOS_883	GCA_016028275.1	1281	1281	type	True	85.0379	674	818	95	below_threshold
Staphylococcus simulans	strain=NCTC 11046	GCA_002902285.1	1286	1286	type	True	80.8434	465	818	95	below_threshold
Staphylococcus simulans	strain=NCTC11046	GCA_900474685.1	1286	1286	type	True	80.7842	475	818	95	below_threshold
Macrococcus armenti	strain=JEK37	GCA_020097135.1	2875764	2875764	type	True	78.3406	59	818	95	below_threshold
Staphylococcus schleiferi	strain=NCTC12218	GCA_900458895.2	1295	1295	type	True	77.8716	150	818	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-25 18:16:34,051] [INFO] DFAST Taxonomy check result was written to GCF_003970555.1_ASM397055v1_genomic.fna/tc_result.tsv
[2024-01-25 18:16:34,052] [INFO] ===== Taxonomy check completed =====
[2024-01-25 18:16:34,052] [INFO] ===== Start completeness check using CheckM =====
[2024-01-25 18:16:34,052] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stg72976acc-0a84-45d3-b399-661bd0ac590f/dqc_reference/checkm_data
[2024-01-25 18:16:34,053] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-25 18:16:34,080] [INFO] Task started: CheckM
[2024-01-25 18:16:34,081] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_003970555.1_ASM397055v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_003970555.1_ASM397055v1_genomic.fna/checkm_input GCF_003970555.1_ASM397055v1_genomic.fna/checkm_result
[2024-01-25 18:16:53,476] [INFO] Task succeeded: CheckM
[2024-01-25 18:16:53,477] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-25 18:16:53,509] [INFO] ===== Completeness check finished =====
[2024-01-25 18:16:53,509] [INFO] ===== Start GTDB Search =====
[2024-01-25 18:16:53,509] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_003970555.1_ASM397055v1_genomic.fna/markers.fasta)
[2024-01-25 18:16:53,510] [INFO] Task started: Blastn
[2024-01-25 18:16:53,510] [INFO] Running command: blastn -query GCF_003970555.1_ASM397055v1_genomic.fna/markers.fasta -db /var/lib/cwl/stg72976acc-0a84-45d3-b399-661bd0ac590f/dqc_reference/reference_markers_gtdb.fasta -out GCF_003970555.1_ASM397055v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-25 18:16:54,282] [INFO] Task succeeded: Blastn
[2024-01-25 18:16:54,286] [INFO] Selected 7 target genomes.
[2024-01-25 18:16:54,286] [INFO] Target genome list was writen to GCF_003970555.1_ASM397055v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-25 18:16:54,307] [INFO] Task started: fastANI
[2024-01-25 18:16:54,308] [INFO] Running command: fastANI --query /var/lib/cwl/stga516cc9a-9f75-4ea0-92e3-351fb59007a7/GCF_003970555.1_ASM397055v1_genomic.fna.gz --refList GCF_003970555.1_ASM397055v1_genomic.fna/target_genomes_gtdb.txt --output GCF_003970555.1_ASM397055v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-25 18:16:58,853] [INFO] Task succeeded: fastANI
[2024-01-25 18:16:58,859] [INFO] Found 7 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-25 18:16:58,859] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_900186985.1	s__Staphylococcus piscifermentans	99.9977	809	818	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	100.00	100.00	0.99	0.99	3	conclusive
GCF_003718735.1	s__Staphylococcus debuckii	94.5692	752	818	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_900458435.1	s__Staphylococcus carnosus	85.0978	667	818	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	99.71	99.56	0.96	0.93	12	-
GCF_001618885.1	s__Staphylococcus condimenti	84.8468	682	818	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	99.75	99.00	0.98	0.93	11	-
GCF_002994445.1	s__Staphylococcus simulans_B	80.8912	465	818	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	96.89	96.89	0.92	0.92	2	-
GCF_002902285.1	s__Staphylococcus simulans	80.8345	466	818	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	98.92	98.61	0.93	0.91	77	-
GCF_003043455.1	s__Staphylococcus simulans_A	80.7321	465	818	d__Bacteria;p__Firmicutes;c__Bacilli;o__Staphylococcales;f__Staphylococcaceae;g__Staphylococcus	95.0	98.25	98.25	0.90	0.90	2	-
--------------------------------------------------------------------------------
[2024-01-25 18:16:58,861] [INFO] GTDB search result was written to GCF_003970555.1_ASM397055v1_genomic.fna/result_gtdb.tsv
[2024-01-25 18:16:58,861] [INFO] ===== GTDB Search completed =====
[2024-01-25 18:16:58,864] [INFO] DFAST_QC result json was written to GCF_003970555.1_ASM397055v1_genomic.fna/dqc_result.json
[2024-01-25 18:16:58,864] [INFO] DFAST_QC completed!
[2024-01-25 18:16:58,864] [INFO] Total running time: 0h0m38s