[2024-01-24 11:05:28,117] [INFO] DFAST_QC pipeline started.
[2024-01-24 11:05:28,119] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 11:05:28,119] [INFO] DQC Reference Directory: /var/lib/cwl/stgcf6a480b-6457-4425-b13f-9a547c35b338/dqc_reference
[2024-01-24 11:05:29,545] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 11:05:29,546] [INFO] Task started: Prodigal
[2024-01-24 11:05:29,548] [INFO] Running command: gunzip -c /var/lib/cwl/stga25ef606-b73a-47b8-b2a3-09113ee63a59/GCF_004307455.1_ASM430745v1_genomic.fna.gz | prodigal -d GCF_004307455.1_ASM430745v1_genomic.fna/cds.fna -a GCF_004307455.1_ASM430745v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 11:05:42,516] [INFO] Task succeeded: Prodigal
[2024-01-24 11:05:42,517] [INFO] Task started: HMMsearch
[2024-01-24 11:05:42,517] [INFO] Running command: hmmsearch --tblout GCF_004307455.1_ASM430745v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgcf6a480b-6457-4425-b13f-9a547c35b338/dqc_reference/reference_markers.hmm GCF_004307455.1_ASM430745v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 11:05:42,782] [INFO] Task succeeded: HMMsearch
[2024-01-24 11:05:42,784] [INFO] Found 6/6 markers.
[2024-01-24 11:05:42,814] [INFO] Query marker FASTA was written to GCF_004307455.1_ASM430745v1_genomic.fna/markers.fasta
[2024-01-24 11:05:42,815] [INFO] Task started: Blastn
[2024-01-24 11:05:42,815] [INFO] Running command: blastn -query GCF_004307455.1_ASM430745v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgcf6a480b-6457-4425-b13f-9a547c35b338/dqc_reference/reference_markers.fasta -out GCF_004307455.1_ASM430745v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:05:43,461] [INFO] Task succeeded: Blastn
[2024-01-24 11:05:43,464] [INFO] Selected 10 target genomes.
[2024-01-24 11:05:43,465] [INFO] Target genome list was writen to GCF_004307455.1_ASM430745v1_genomic.fna/target_genomes.txt
[2024-01-24 11:05:43,482] [INFO] Task started: fastANI
[2024-01-24 11:05:43,482] [INFO] Running command: fastANI --query /var/lib/cwl/stga25ef606-b73a-47b8-b2a3-09113ee63a59/GCF_004307455.1_ASM430745v1_genomic.fna.gz --refList GCF_004307455.1_ASM430745v1_genomic.fna/target_genomes.txt --output GCF_004307455.1_ASM430745v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 11:05:50,799] [INFO] Task succeeded: fastANI
[2024-01-24 11:05:50,800] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgcf6a480b-6457-4425-b13f-9a547c35b338/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 11:05:50,800] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgcf6a480b-6457-4425-b13f-9a547c35b338/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 11:05:50,808] [INFO] Found 7 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 11:05:50,808] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 11:05:50,808] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Aquirufa nivalisilvae	strain=59G-WUEMPEL	GCA_004307455.1	2516557	2516557	type	True	100.0	1020	1021	95	conclusive
Aquirufa aurantiipilula	strain=15D-MOB	GCA_019923745.1	2696561	2696561	type	True	86.2855	872	1021	95	below_threshold
Aquirufa beregesia	strain=50C-KIRBA	GCA_011090385.1	2516556	2516556	type	True	86.2078	877	1021	95	below_threshold
Aquirufa ecclesiirivi	strain=50A-KIRBA	GCA_011250515.1	2715124	2715124	type	True	85.7413	887	1021	95	below_threshold
Aquirufa rosea	strain=CAR-16	GCA_004118285.1	2509241	2509241	type	True	80.7179	649	1021	95	below_threshold
Aquirufa lenticrescens	strain=9H-EGSE	GCA_019916085.1	2696560	2696560	type	True	77.9582	185	1021	95	below_threshold
Sandaracinomonas limnophila	strain=FSY-15	GCA_004005965.1	1862386	1862386	type	True	77.2642	185	1021	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 11:05:50,809] [INFO] DFAST Taxonomy check result was written to GCF_004307455.1_ASM430745v1_genomic.fna/tc_result.tsv
[2024-01-24 11:05:50,810] [INFO] ===== Taxonomy check completed =====
[2024-01-24 11:05:50,810] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 11:05:50,810] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgcf6a480b-6457-4425-b13f-9a547c35b338/dqc_reference/checkm_data
[2024-01-24 11:05:50,811] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 11:05:50,843] [INFO] Task started: CheckM
[2024-01-24 11:05:50,843] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_004307455.1_ASM430745v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_004307455.1_ASM430745v1_genomic.fna/checkm_input GCF_004307455.1_ASM430745v1_genomic.fna/checkm_result
[2024-01-24 11:06:34,040] [INFO] Task succeeded: CheckM
[2024-01-24 11:06:34,041] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 11:06:34,062] [INFO] ===== Completeness check finished =====
[2024-01-24 11:06:34,063] [INFO] ===== Start GTDB Search =====
[2024-01-24 11:06:34,064] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_004307455.1_ASM430745v1_genomic.fna/markers.fasta)
[2024-01-24 11:06:34,064] [INFO] Task started: Blastn
[2024-01-24 11:06:34,064] [INFO] Running command: blastn -query GCF_004307455.1_ASM430745v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgcf6a480b-6457-4425-b13f-9a547c35b338/dqc_reference/reference_markers_gtdb.fasta -out GCF_004307455.1_ASM430745v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:06:34,846] [INFO] Task succeeded: Blastn
[2024-01-24 11:06:34,849] [INFO] Selected 11 target genomes.
[2024-01-24 11:06:34,850] [INFO] Target genome list was writen to GCF_004307455.1_ASM430745v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 11:06:34,875] [INFO] Task started: fastANI
[2024-01-24 11:06:34,875] [INFO] Running command: fastANI --query /var/lib/cwl/stga25ef606-b73a-47b8-b2a3-09113ee63a59/GCF_004307455.1_ASM430745v1_genomic.fna.gz --refList GCF_004307455.1_ASM430745v1_genomic.fna/target_genomes_gtdb.txt --output GCF_004307455.1_ASM430745v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 11:06:42,184] [INFO] Task succeeded: fastANI
[2024-01-24 11:06:42,200] [INFO] Found 10 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 11:06:42,200] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCA_004307455.1	s__Allopseudarcicella nivalisilvae	100.0	1020	1021	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Spirosomaceae;g__Allopseudarcicella	95.0	97.41	97.41	0.94	0.94	2	conclusive
GCF_011090385.1	s__Allopseudarcicella beregesia	86.2189	876	1021	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Spirosomaceae;g__Allopseudarcicella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_011250515.1	s__Allopseudarcicella ecclesiirivi	85.7317	888	1021	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Spirosomaceae;g__Allopseudarcicella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_004118285.1	s__Allopseudarcicella rosea	80.7214	649	1021	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Spirosomaceae;g__Allopseudarcicella	95.0	N/A	N/A	N/A	N/A	1	-
GCF_004307425.1	s__Aquirufa antheringensis	78.4142	175	1021	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Spirosomaceae;g__Aquirufa	95.0	97.17	97.17	0.93	0.93	2	-
GCA_002454335.1	s__Aquirufa sp002454335	77.8645	183	1021	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Spirosomaceae;g__Aquirufa	95.0	99.80	99.80	0.91	0.91	2	-
GCA_017984215.1	s__Aquirufa sp017984215	77.826	180	1021	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Spirosomaceae;g__Aquirufa	95.0	99.80	99.80	0.94	0.94	2	-
GCA_903907915.1	s__Aquirufa sp903907915	77.518	145	1021	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Spirosomaceae;g__Aquirufa	95.0	N/A	N/A	N/A	N/A	1	-
GCA_004005965.1	s__UBA6715 sp004005965	77.2706	186	1021	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Spirosomaceae;g__UBA6715	95.0	N/A	N/A	N/A	N/A	1	-
GCA_013298025.1	s__Flectobacillus sp013298025	76.2376	61	1021	d__Bacteria;p__Bacteroidota;c__Bacteroidia;o__Cytophagales;f__Spirosomaceae;g__Flectobacillus	95.0	N/A	N/A	N/A	N/A	1	-
--------------------------------------------------------------------------------
[2024-01-24 11:06:42,202] [INFO] GTDB search result was written to GCF_004307455.1_ASM430745v1_genomic.fna/result_gtdb.tsv
[2024-01-24 11:06:42,203] [INFO] ===== GTDB Search completed =====
[2024-01-24 11:06:42,206] [INFO] DFAST_QC result json was written to GCF_004307455.1_ASM430745v1_genomic.fna/dqc_result.json
[2024-01-24 11:06:42,206] [INFO] DFAST_QC completed!
[2024-01-24 11:06:42,206] [INFO] Total running time: 0h1m14s