[2024-01-24 14:54:56,449] [INFO] DFAST_QC pipeline started.
[2024-01-24 14:54:56,451] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 14:54:56,451] [INFO] DQC Reference Directory: /var/lib/cwl/stgd6711774-a758-40cf-9ca6-d16ca507b293/dqc_reference
[2024-01-24 14:54:57,844] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 14:54:57,845] [INFO] Task started: Prodigal
[2024-01-24 14:54:57,846] [INFO] Running command: gunzip -c /var/lib/cwl/stg0963a49f-eb2c-4d7e-a182-1c4af7f5660b/GCF_004354515.1_ASM435451v1_genomic.fna.gz | prodigal -d GCF_004354515.1_ASM435451v1_genomic.fna/cds.fna -a GCF_004354515.1_ASM435451v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 14:55:02,518] [INFO] Task succeeded: Prodigal
[2024-01-24 14:55:02,519] [INFO] Task started: HMMsearch
[2024-01-24 14:55:02,519] [INFO] Running command: hmmsearch --tblout GCF_004354515.1_ASM435451v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stgd6711774-a758-40cf-9ca6-d16ca507b293/dqc_reference/reference_markers.hmm GCF_004354515.1_ASM435451v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 14:55:02,785] [INFO] Task succeeded: HMMsearch
[2024-01-24 14:55:02,787] [INFO] Found 6/6 markers.
[2024-01-24 14:55:02,813] [INFO] Query marker FASTA was written to GCF_004354515.1_ASM435451v1_genomic.fna/markers.fasta
[2024-01-24 14:55:02,813] [INFO] Task started: Blastn
[2024-01-24 14:55:02,813] [INFO] Running command: blastn -query GCF_004354515.1_ASM435451v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgd6711774-a758-40cf-9ca6-d16ca507b293/dqc_reference/reference_markers.fasta -out GCF_004354515.1_ASM435451v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:55:03,390] [INFO] Task succeeded: Blastn
[2024-01-24 14:55:03,393] [INFO] Selected 10 target genomes.
[2024-01-24 14:55:03,394] [INFO] Target genome list was writen to GCF_004354515.1_ASM435451v1_genomic.fna/target_genomes.txt
[2024-01-24 14:55:03,400] [INFO] Task started: fastANI
[2024-01-24 14:55:03,400] [INFO] Running command: fastANI --query /var/lib/cwl/stg0963a49f-eb2c-4d7e-a182-1c4af7f5660b/GCF_004354515.1_ASM435451v1_genomic.fna.gz --refList GCF_004354515.1_ASM435451v1_genomic.fna/target_genomes.txt --output GCF_004354515.1_ASM435451v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 14:55:10,505] [INFO] Task succeeded: fastANI
[2024-01-24 14:55:10,506] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stgd6711774-a758-40cf-9ca6-d16ca507b293/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 14:55:10,507] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stgd6711774-a758-40cf-9ca6-d16ca507b293/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 14:55:10,517] [INFO] Found 10 fastANI hits (5 hits with ANI > threshold)
[2024-01-24 14:55:10,517] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 14:55:10,517] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Lactococcus cremoris	strain=ATCC 19257	GCA_004354515.1	1359	1359	type	True	100.0	734	749	95	conclusive
Lactococcus cremoris	strain=LMG6897	GCA_001622295.1	1359	1359	type	True	99.8317	630	749	95	conclusive
Lactococcus cremoris	strain=ATCC 19257	GCA_002441765.1	1359	1359	type	True	99.7872	635	749	95	conclusive
Lactococcus cremoris subsp. cremoris	strain=NBRC 100676	GCA_001591705.1	2816960	1359	type	True	98.9938	642	749	95	conclusive
Lactococcus cremoris subsp. tructae	strain=DSM 21502	GCA_002441825.1	542833	1359	type	True	97.5497	620	749	95	conclusive
Lactococcus lactis subsp. hordniae	strain=NBRC 100931	GCA_001591725.1	203404	1358	type	True	87.2372	521	749	95	below_threshold
Lactococcus lactis subsp. hordniae	strain=CCUG 32210T	GCA_008692945.1	203404	1358	type	True	87.1551	530	749	95	below_threshold
Lactococcus lactis subsp. hordniae	strain=DSM 20450	GCA_002441785.1	203404	1358	type	True	87.1217	528	749	95	below_threshold
Lactococcus paracarnosus	strain=TMW 2.1615	GCA_006770285.1	2749962	2749962	type	True	79.4821	62	749	95	below_threshold
Lactococcus carnosus	strain=TMW 2.1612	GCA_006770265.1	2749961	2749961	type	True	78.9601	58	749	95	below_threshold
--------------------------------------------------------------------------------
[2024-01-24 14:55:10,518] [INFO] DFAST Taxonomy check result was written to GCF_004354515.1_ASM435451v1_genomic.fna/tc_result.tsv
[2024-01-24 14:55:10,519] [INFO] ===== Taxonomy check completed =====
[2024-01-24 14:55:10,519] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 14:55:10,519] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stgd6711774-a758-40cf-9ca6-d16ca507b293/dqc_reference/checkm_data
[2024-01-24 14:55:10,520] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 14:55:10,550] [INFO] Task started: CheckM
[2024-01-24 14:55:10,550] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_004354515.1_ASM435451v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_004354515.1_ASM435451v1_genomic.fna/checkm_input GCF_004354515.1_ASM435451v1_genomic.fna/checkm_result
[2024-01-24 14:55:32,948] [INFO] Task succeeded: CheckM
[2024-01-24 14:55:32,949] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 14:55:32,976] [INFO] ===== Completeness check finished =====
[2024-01-24 14:55:32,976] [INFO] ===== Start GTDB Search =====
[2024-01-24 14:55:32,977] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_004354515.1_ASM435451v1_genomic.fna/markers.fasta)
[2024-01-24 14:55:32,977] [INFO] Task started: Blastn
[2024-01-24 14:55:32,977] [INFO] Running command: blastn -query GCF_004354515.1_ASM435451v1_genomic.fna/markers.fasta -db /var/lib/cwl/stgd6711774-a758-40cf-9ca6-d16ca507b293/dqc_reference/reference_markers_gtdb.fasta -out GCF_004354515.1_ASM435451v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 14:55:33,809] [INFO] Task succeeded: Blastn
[2024-01-24 14:55:33,813] [INFO] Selected 16 target genomes.
[2024-01-24 14:55:33,813] [INFO] Target genome list was writen to GCF_004354515.1_ASM435451v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 14:55:33,830] [INFO] Task started: fastANI
[2024-01-24 14:55:33,830] [INFO] Running command: fastANI --query /var/lib/cwl/stg0963a49f-eb2c-4d7e-a182-1c4af7f5660b/GCF_004354515.1_ASM435451v1_genomic.fna.gz --refList GCF_004354515.1_ASM435451v1_genomic.fna/target_genomes_gtdb.txt --output GCF_004354515.1_ASM435451v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 14:55:41,459] [INFO] Task succeeded: fastANI
[2024-01-24 14:55:41,476] [INFO] Found 12 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 14:55:41,476] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_001591705.1	s__Lactococcus cremoris	98.9938	642	749	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus	95.0	98.76	97.45	0.91	0.82	70	conclusive
GCF_900099625.1	s__Lactococcus lactis	87.6854	562	749	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus	95.0	98.37	97.03	0.87	0.82	223	-
GCF_014905395.1	s__Lactococcus taiwanensis	82.0171	302	749	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus	95.0	98.53	98.39	0.94	0.93	6	-
GCF_004210295.1	s__Lactococcus sp004210295	81.3891	331	749	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_009601015.1	s__Lactococcus hircilactis	80.0221	167	749	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_000187935.1	s__Streptococcus parauberis	79.8386	54	749	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Streptococcus	95.0	98.87	98.45	0.89	0.84	24	-
GCF_006965445.1	s__Lactococcus sp006965445	79.5029	280	749	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus	95.0	98.72	98.72	0.90	0.90	2	-
GCF_002441655.1	s__Lactococcus fujiensis	78.7374	126	749	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus	95.0	99.84	99.84	0.99	0.99	2	-
GCF_018403745.1	s__Lactococcus formosensis	78.6652	120	749	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus	95.0	98.20	97.52	0.88	0.85	8	-
GCF_009601055.1	s__Lactococcus sp009601055	78.6497	159	749	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus	95.0	100.00	99.99	0.99	0.98	3	-
GCF_018403765.1	s__Lactococcus nasutitermitis	78.1697	154	749	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus	95.0	N/A	N/A	N/A	N/A	1	-
GCF_016758115.1	s__Lactococcus sp002492185	78.0195	113	749	d__Bacteria;p__Firmicutes;c__Bacilli;o__Lactobacillales;f__Streptococcaceae;g__Lactococcus	95.0	98.71	98.67	0.89	0.88	5	-
--------------------------------------------------------------------------------
[2024-01-24 14:55:41,479] [INFO] GTDB search result was written to GCF_004354515.1_ASM435451v1_genomic.fna/result_gtdb.tsv
[2024-01-24 14:55:41,480] [INFO] ===== GTDB Search completed =====
[2024-01-24 14:55:41,484] [INFO] DFAST_QC result json was written to GCF_004354515.1_ASM435451v1_genomic.fna/dqc_result.json
[2024-01-24 14:55:41,485] [INFO] DFAST_QC completed!
[2024-01-24 14:55:41,485] [INFO] Total running time: 0h0m45s