[2024-01-24 11:05:28,110] [INFO] DFAST_QC pipeline started.
[2024-01-24 11:05:28,111] [INFO] DFAST_QC version: 0.5.7
[2024-01-24 11:05:28,112] [INFO] DQC Reference Directory: /var/lib/cwl/stga1f8509b-0513-4fb1-b47d-e387c8e24b40/dqc_reference
[2024-01-24 11:05:29,467] [INFO] ===== Start taxonomy check using ANI =====
[2024-01-24 11:05:29,468] [INFO] Task started: Prodigal
[2024-01-24 11:05:29,468] [INFO] Running command: gunzip -c /var/lib/cwl/stgb38a07d9-c093-464a-aada-1e6b2ed8b75e/GCF_004363045.1_ASM436304v1_genomic.fna.gz | prodigal -d GCF_004363045.1_ASM436304v1_genomic.fna/cds.fna -a GCF_004363045.1_ASM436304v1_genomic.fna/protein.faa -g 11 -q > /dev/null
[2024-01-24 11:05:40,993] [INFO] Task succeeded: Prodigal
[2024-01-24 11:05:40,994] [INFO] Task started: HMMsearch
[2024-01-24 11:05:40,994] [INFO] Running command: hmmsearch --tblout GCF_004363045.1_ASM436304v1_genomic.fna/hmmer_result.tsv -E 1E-50 /var/lib/cwl/stga1f8509b-0513-4fb1-b47d-e387c8e24b40/dqc_reference/reference_markers.hmm GCF_004363045.1_ASM436304v1_genomic.fna/protein.faa > /dev/null
[2024-01-24 11:05:41,338] [INFO] Task succeeded: HMMsearch
[2024-01-24 11:05:41,339] [INFO] Found 6/6 markers.
[2024-01-24 11:05:41,374] [INFO] Query marker FASTA was written to GCF_004363045.1_ASM436304v1_genomic.fna/markers.fasta
[2024-01-24 11:05:41,375] [INFO] Task started: Blastn
[2024-01-24 11:05:41,375] [INFO] Running command: blastn -query GCF_004363045.1_ASM436304v1_genomic.fna/markers.fasta -db /var/lib/cwl/stga1f8509b-0513-4fb1-b47d-e387c8e24b40/dqc_reference/reference_markers.fasta -out GCF_004363045.1_ASM436304v1_genomic.fna/blast.markers.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:05:42,062] [INFO] Task succeeded: Blastn
[2024-01-24 11:05:42,065] [INFO] Selected 23 target genomes.
[2024-01-24 11:05:42,066] [INFO] Target genome list was writen to GCF_004363045.1_ASM436304v1_genomic.fna/target_genomes.txt
[2024-01-24 11:05:42,074] [INFO] Task started: fastANI
[2024-01-24 11:05:42,075] [INFO] Running command: fastANI --query /var/lib/cwl/stgb38a07d9-c093-464a-aada-1e6b2ed8b75e/GCF_004363045.1_ASM436304v1_genomic.fna.gz --refList GCF_004363045.1_ASM436304v1_genomic.fna/target_genomes.txt --output GCF_004363045.1_ASM436304v1_genomic.fna/fastani_result.tsv --threads 1
[2024-01-24 11:06:00,867] [INFO] Task succeeded: fastANI
[2024-01-24 11:06:00,867] [INFO] Loading species specific ANI threshold from /var/lib/cwl/stga1f8509b-0513-4fb1-b47d-e387c8e24b40/dqc_reference/prokaryote_ANI_species_specific_threshold.txt
[2024-01-24 11:06:00,868] [WARNING] Species-specific ANI threshold file not found. Will use the default threshold for all species. [/var/lib/cwl/stga1f8509b-0513-4fb1-b47d-e387c8e24b40/dqc_reference/prokaryote_ANI_species_specific_threshold.txt]
[2024-01-24 11:06:00,871] [INFO] Found 1 fastANI hits (1 hits with ANI > threshold)
[2024-01-24 11:06:00,871] [INFO] The taxonomy check result is classified as 'conclusive'.
[2024-01-24 11:06:00,871] [INFO] DFAST Taxonomy check final result
--------------------------------------------------------------------------------
organism_name	strain	accession	taxid	species_taxid	relation_to_type	validated	ani	matched_fragments	total_fragments	ani_threshold	status
Aureibacillus halotolerans	strain=DSM 28697	GCA_004363045.1	1508390	1508390	type	True	100.0	1437	1441	95	conclusive
--------------------------------------------------------------------------------
[2024-01-24 11:06:00,873] [INFO] DFAST Taxonomy check result was written to GCF_004363045.1_ASM436304v1_genomic.fna/tc_result.tsv
[2024-01-24 11:06:00,874] [INFO] ===== Taxonomy check completed =====
[2024-01-24 11:06:00,874] [INFO] ===== Start completeness check using CheckM =====
[2024-01-24 11:06:00,874] [INFO] Setting CHECKM_DATA_PATH to /var/lib/cwl/stga1f8509b-0513-4fb1-b47d-e387c8e24b40/dqc_reference/checkm_data
[2024-01-24 11:06:00,875] [INFO] Selected 'Prokaryote' markers (life, taxid=0) for CheckM
[2024-01-24 11:06:00,921] [INFO] Task started: CheckM
[2024-01-24 11:06:00,922] [INFO] Running command: checkm taxonomy_wf --tab_table -f GCF_004363045.1_ASM436304v1_genomic.fna/cc_result.tsv -t 1 life "Prokaryote" GCF_004363045.1_ASM436304v1_genomic.fna/checkm_input GCF_004363045.1_ASM436304v1_genomic.fna/checkm_result
[2024-01-24 11:06:40,761] [INFO] Task succeeded: CheckM
[2024-01-24 11:06:40,763] [INFO] Completeness check finished.
--------------------------------------------------------------------------------
Completeness: 100.00%
Contamintation: 0.00%
Strain heterogeneity: 0.00%
--------------------------------------------------------------------------------
[2024-01-24 11:06:40,785] [INFO] ===== Completeness check finished =====
[2024-01-24 11:06:40,785] [INFO] ===== Start GTDB Search =====
[2024-01-24 11:06:40,786] [INFO] Query marker FASTA already exists. Will reuse it. (GCF_004363045.1_ASM436304v1_genomic.fna/markers.fasta)
[2024-01-24 11:06:40,786] [INFO] Task started: Blastn
[2024-01-24 11:06:40,786] [INFO] Running command: blastn -query GCF_004363045.1_ASM436304v1_genomic.fna/markers.fasta -db /var/lib/cwl/stga1f8509b-0513-4fb1-b47d-e387c8e24b40/dqc_reference/reference_markers_gtdb.fasta -out GCF_004363045.1_ASM436304v1_genomic.fna/blast.markers.gtdb.tsv -outfmt 6 -max_hsps 1 -num_alignments 5
[2024-01-24 11:06:41,703] [INFO] Task succeeded: Blastn
[2024-01-24 11:06:41,708] [INFO] Selected 28 target genomes.
[2024-01-24 11:06:41,708] [INFO] Target genome list was writen to GCF_004363045.1_ASM436304v1_genomic.fna/target_genomes_gtdb.txt
[2024-01-24 11:06:41,742] [INFO] Task started: fastANI
[2024-01-24 11:06:41,743] [INFO] Running command: fastANI --query /var/lib/cwl/stgb38a07d9-c093-464a-aada-1e6b2ed8b75e/GCF_004363045.1_ASM436304v1_genomic.fna.gz --refList GCF_004363045.1_ASM436304v1_genomic.fna/target_genomes_gtdb.txt --output GCF_004363045.1_ASM436304v1_genomic.fna/fastani_result_gtdb.tsv --threads 1
[2024-01-24 11:07:02,951] [INFO] Task succeeded: fastANI
[2024-01-24 11:07:02,956] [INFO] Found 1 fastANI hits (1 hits with ANI > circumscription radius)
[2024-01-24 11:07:02,957] [INFO] GTDB search result
--------------------------------------------------------------------------------
accession	gtdb_species	ani	matched_fragments	total_fragments	gtdb_taxonomy	ani_circumscription_radius	mean_intra_species_ani	min_intra_species_ani	mean_intra_species_af	min_intra_species_af	num_clustered_genomes	status
GCF_004363045.1	s__Aureibacillus halotolerans	100.0	1437	1441	d__Bacteria;p__Firmicutes;c__Bacilli;o__Bacillales_D;f__DSM-28697;g__Aureibacillus	95.0	N/A	N/A	N/A	N/A	1	conclusive
--------------------------------------------------------------------------------
[2024-01-24 11:07:02,958] [INFO] GTDB search result was written to GCF_004363045.1_ASM436304v1_genomic.fna/result_gtdb.tsv
[2024-01-24 11:07:02,959] [INFO] ===== GTDB Search completed =====
[2024-01-24 11:07:02,961] [INFO] DFAST_QC result json was written to GCF_004363045.1_ASM436304v1_genomic.fna/dqc_result.json
[2024-01-24 11:07:02,962] [INFO] DFAST_QC completed!
[2024-01-24 11:07:02,962] [INFO] Total running time: 0h1m35s